FastQCFastQC Report
Mon 6 Feb 2017
SRR3203955_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203955_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526758
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28200.036.086381
TCAACGC34950.027.5355054
ATCAACG35200.027.241953
CAACGCA35400.027.0880375
TGACGCG658.6696586E-426.53338410
GGTATCA14550.026.3166351
AACGCAG38300.025.3972286
TATCAAC38750.025.2803252
ACGCAGA43250.021.7727137
GTACATG62300.020.3765281
CGCAGAG45500.020.0895638
TCGCAAT3400.019.27686749
TACATGG63050.018.9289722
ACATGGG63600.018.1686943
AGAGTAC47050.018.03480711
CGTGCCG2853.2923708E-1016.94412610
GCACCGT4800.016.5280886
CAGAGTA52400.016.52260210
GCAGAGT54750.016.1284229
GTATAGG5650.015.8743261
Produced by FastQC (version 0.11.5)