##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203955_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2526758
Sequences flagged as poor quality	0
Sequence length	75
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.440327486842826	35.0	31.0	35.0	15.0	35.0
2	31.714850413058947	35.0	31.0	35.0	27.0	35.0
3	31.809775213930262	35.0	31.0	35.0	27.0	35.0
4	31.980324985613976	35.0	31.0	35.0	27.0	35.0
5	32.09893586960049	35.0	32.0	35.0	27.0	35.0
6	35.766429551227304	39.0	36.0	40.0	27.0	40.0
7	35.50957076221783	39.0	35.0	40.0	27.0	40.0
8	35.56907309683001	39.0	35.0	40.0	27.0	40.0
9	35.510680484636836	39.0	35.0	40.0	16.0	40.0
10	35.61337532126147	39.0	35.0	40.0	27.0	40.0
11	35.684703085930664	39.0	35.0	40.0	27.0	40.0
12	35.27417188349656	39.0	35.0	40.0	16.0	40.0
13	35.335996165837805	39.0	35.0	40.0	16.0	40.0
14	35.31521855278582	39.0	35.0	40.0	16.0	40.0
15	35.37396220769856	39.0	35.0	40.0	16.0	40.0
16	35.51926896046238	39.0	35.0	40.0	27.0	40.0
17	35.559255773603965	39.0	35.0	40.0	27.0	40.0
18	35.61109057535387	39.0	35.0	40.0	27.0	40.0
19	35.652221542387515	39.0	35.0	40.0	27.0	40.0
20	35.594148311789255	39.0	35.0	40.0	27.0	40.0
21	35.62058337205225	39.0	35.0	40.0	27.0	40.0
22	35.67661762622301	39.0	35.0	40.0	27.0	40.0
23	35.694145224829605	39.0	36.0	40.0	27.0	40.0
24	35.721863352169066	39.0	36.0	40.0	27.0	40.0
25	35.744642739827086	39.0	36.0	40.0	27.0	40.0
26	35.752913812878006	39.0	36.0	40.0	27.0	40.0
27	35.74902859711931	39.0	36.0	40.0	27.0	40.0
28	35.6177461395195	39.0	35.0	40.0	27.0	40.0
29	35.70603437289998	39.0	36.0	40.0	27.0	40.0
30	35.72218550411238	39.0	36.0	40.0	27.0	40.0
31	35.72134529701697	39.0	36.0	40.0	27.0	40.0
32	35.65474018485347	39.0	36.0	40.0	27.0	40.0
33	35.54555481767546	39.0	35.0	40.0	27.0	40.0
34	35.58267946514862	39.0	35.0	40.0	27.0	40.0
35	35.57807870797283	39.0	35.0	40.0	27.0	40.0
36	35.59861569647746	39.0	35.0	40.0	27.0	40.0
37	35.57210979444806	39.0	35.0	40.0	27.0	40.0
38	35.58275822219619	39.0	35.0	40.0	27.0	40.0
39	35.542582629598876	39.0	35.0	40.0	27.0	40.0
40	35.46230466075501	39.0	35.0	40.0	27.0	40.0
41	35.433480768637125	39.0	35.0	40.0	27.0	40.0
42	35.451212581497714	39.0	35.0	40.0	27.0	40.0
43	35.405150790063786	39.0	35.0	40.0	27.0	40.0
44	35.28266141830757	39.0	35.0	40.0	16.0	40.0
45	35.31137133037671	39.0	35.0	40.0	27.0	40.0
46	35.274999030378055	39.0	35.0	40.0	16.0	40.0
47	35.20011651293871	39.0	35.0	40.0	16.0	40.0
48	35.24558267946515	39.0	35.0	40.0	16.0	40.0
49	35.20423641678388	39.0	35.0	40.0	16.0	40.0
50	35.094828630205186	39.0	34.0	40.0	16.0	40.0
51	35.13863337921558	39.0	35.0	40.0	16.0	40.0
52	35.17282066584928	39.0	35.0	40.0	16.0	40.0
53	35.121426349496076	39.0	35.0	40.0	16.0	40.0
54	35.150349974156605	39.0	35.0	40.0	16.0	40.0
55	35.13686233505543	39.0	35.0	40.0	16.0	40.0
56	35.0924690848906	39.0	35.0	40.0	16.0	40.0
57	34.9867640668398	39.0	34.0	40.0	16.0	40.0
58	35.007464901664505	39.0	34.0	40.0	16.0	40.0
59	34.970221920737956	39.0	34.0	40.0	16.0	40.0
60	34.92993986760901	39.0	34.0	40.0	16.0	40.0
61	34.90597952000152	39.0	34.0	40.0	16.0	40.0
62	34.872532707920584	39.0	34.0	40.0	16.0	40.0
63	34.838247667564524	39.0	34.0	40.0	16.0	40.0
64	34.83107483977492	39.0	34.0	40.0	16.0	40.0
65	34.75955789988594	39.0	34.0	40.0	16.0	40.0
66	34.72403016038734	39.0	34.0	40.0	16.0	40.0
67	34.72068437103989	39.0	34.0	40.0	16.0	40.0
68	34.68986464077684	39.0	34.0	40.0	16.0	40.0
69	34.66606418184884	39.0	34.0	40.0	16.0	40.0
70	34.566159481834035	39.0	34.0	40.0	16.0	40.0
71	34.45293336362248	39.0	34.0	40.0	16.0	40.0
72	34.449300645332876	39.0	34.0	40.0	16.0	40.0
73	34.40701127690107	39.0	34.0	40.0	16.0	40.0
74	34.37449530188486	39.0	34.0	40.0	16.0	40.0
75	34.297204955915845	39.0	34.0	40.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6071.0
3	1178.0
4	1413.0
5	1309.0
6	1372.0
7	1451.0
8	1526.0
9	1633.0
10	1823.0
11	1939.0
12	2180.0
13	2559.0
14	2792.0
15	3197.0
16	3623.0
17	4089.0
18	5113.0
19	9741.0
20	18142.0
21	25013.0
22	29620.0
23	33672.0
24	37514.0
25	40693.0
26	44544.0
27	48385.0
28	52716.0
29	57652.0
30	63524.0
31	70623.0
32	79198.0
33	89104.0
34	102489.0
35	122081.0
36	155375.0
37	221167.0
38	398511.0
39	783726.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99556254410444	18.176550744793648	13.036827480247739	24.79105923085417
2	15.167578375135252	19.81329434793518	40.44760123446725	24.571526042462317
3	18.57095139304991	25.122904528253198	29.005626973378533	27.300517105318356
4	12.1439409709992	16.598542480126707	38.63939482926343	32.61812171961066
5	13.585313670719554	37.89405237858156	35.10264932375796	13.417984626940926
6	31.561194225960698	37.939842280107555	17.788644579338424	12.710318914593325
7	28.322736091070063	31.95786854142739	22.15613841927086	17.563256948231686
8	25.953692439085973	35.63416045383056	20.223385064972586	18.188762042110877
9	26.590199773781265	16.055949956426378	19.021647502451756	38.332202767340604
10	13.696008877779352	29.475755098034718	34.64324640507718	22.184989619108755
11	34.94845964670934	23.307178605944852	23.985874389237118	17.75848735810869
12	23.41514304100353	24.69654790842653	30.91946280569805	20.968846244871887
13	27.943594123378652	22.14248455926527	25.911266532054118	24.002654785301957
14	21.939695055877927	20.98194603519609	27.566945469253483	29.511413439672495
15	23.43576234843226	29.11794481307668	23.73484124716336	23.7114515913277
16	23.39349474702366	28.330493066609463	25.337962717442668	22.93804946892421
17	22.361500389036067	28.154971706827485	26.680315249818147	22.8032126543183
18	22.428265785643102	27.84936270113719	27.312983673149542	22.40938784007016
19	23.40192452146189	27.392373943211023	27.12911169174096	22.076589843586127
20	23.474784684564174	26.900795406604033	27.200270069393266	22.42414983943852
21	23.64967282185314	26.71399477116526	27.08332179021497	22.55301061676662
22	23.287073791791695	26.611096116050685	27.168885979583322	22.932944112574294
23	23.24255033525173	26.673310225989194	26.966531816659927	23.117607622099147
24	22.827631296705107	26.698045479622508	27.092582669175286	23.381740554497107
25	23.349841971411585	26.579632873429116	26.94144037537429	23.129084779785007
26	22.90215366885155	27.042835127067967	27.086290020650967	22.968721183429516
27	23.245004072412158	27.054233131942198	26.672439545061298	23.028323250584346
28	23.123206989739288	26.886379499807084	26.916366989834362	23.07404652061927
29	23.3379690496676	26.97694041138882	26.63927451698975	23.045816021953826
30	23.254462833401536	26.953946519611293	26.885162726307783	22.906427920679384
31	23.26890822152339	26.59914404149507	26.887339428627516	23.244608308354024
32	23.032478773194743	26.912747481159656	26.8849252678729	23.169848477772703
33	23.213404642712607	26.828688799184587	26.758915850339005	23.198990707763805
34	23.47047085633052	26.747832598135634	26.763979771707465	23.017716773826383
35	23.31502396154438	26.801429658649408	26.911095922560023	22.972450457246186
36	23.396542130271282	26.77498201252356	26.696224964955096	23.13225089225007
37	23.39173232397588	26.482849946730113	26.85126700005857	23.27415072923543
38	23.23061822988606	26.710570579365424	26.897729476437927	23.16108171431059
39	23.491846637715103	26.86717200640032	26.57113943648538	23.0698419193992
40	23.397044826269266	26.565377331795126	27.094554082212163	22.94302375972344
41	23.146830756955904	26.636941923958112	26.939664128348834	23.27656319073715
42	23.383538038612876	26.66743709834323	26.823924436483317	23.12510042656058
43	23.08442128959029	26.445094025699355	27.124621451378765	23.345863233331592
44	23.271141182369846	26.790262072972315	26.788837311649523	23.149759433008317
45	23.31158055460681	26.826443854045174	26.63489169981561	23.227083891532406
46	23.307569011576135	26.762046106658747	26.520589690313646	23.40979519145147
47	23.251580190070023	26.893052071175934	26.596268016699693	23.25909972205435
48	23.04859549216644	27.01260944364654	26.800915165170213	23.137879899016802
49	23.35656996039985	26.550306756721458	26.67449751816359	23.418625764715102
50	23.502406645047362	26.64446162942653	26.730144683653418	23.122987041872694
51	23.27821074811258	26.889357478355443	26.435925931202835	23.396505842329145
52	23.310320917921505	26.821983320879927	26.464726257266246	23.402969503932322
53	23.128314785770005	26.942151311548013	26.67908579687638	23.250448105805607
54	23.12717454585608	26.852106541516786	26.85064216755842	23.17007674506872
55	23.169627892047888	26.61544860852815	26.707544545027474	23.507378954396483
56	23.09311904965442	27.038750265163387	26.718021295390432	23.150109389791766
57	23.246371718260672	26.662621940384216	26.724123942761185	23.366882398593923
58	23.127209865051306	26.628047704194103	26.82498145830711	23.419760972447477
59	23.231774356068012	26.86111205655346	26.609048331804352	23.298065255574173
60	23.1945414644089	26.90556994639221	26.858591395445018	23.04129719375387
61	23.38927481351081	26.620223787028714	26.846844564900774	23.14365683455971
62	23.24070922547196	26.879922428464003	26.743182807614673	23.13618553844936
63	22.93171827699356	26.770089162919717	26.983923445506292	23.314269114580426
64	23.14934022821008	26.717304380761203	26.658532231873345	23.474823159155374
65	23.298224283937262	26.766583593851106	26.669025475531516	23.266166646680116
66	23.217234955449424	26.906306657869877	26.526954947581388	23.34950343909931
67	23.25618763829847	26.728911449797383	26.588767680733415	23.426133231170727
68	23.289916332233066	26.824582650613856	26.870374328164452	23.015126688988623
69	23.30623703083944	26.821737886314263	26.53737116921225	23.33465391363405
70	23.200171926711093	26.6041941568665	26.785540425336553	23.410093491085853
71	23.306329592862117	26.70734443957641	26.6496008409431	23.336725126618376
72	23.039346444857276	26.884112059282856	26.675859033835774	23.4006824620241
73	23.288937682373675	26.704393669493882	26.615386015785504	23.39128263234694
74	23.169031810712486	26.719122522060427	26.693357649673725	23.418488017553358
75	23.264487766343535	26.78284636433508	26.706343960821904	23.246321908499485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	162.0
1	192.5
2	262.5
3	304.5
4	307.0
5	279.0
6	267.0
7	292.5
8	302.0
9	413.5
10	623.5
11	732.0
12	742.0
13	763.0
14	861.5
15	1067.5
16	1196.0
17	1326.0
18	1696.5
19	2324.0
20	2711.0
21	3335.0
22	5259.5
23	7921.0
24	9282.0
25	11462.5
26	16623.5
27	22335.0
28	25066.0
29	29151.5
30	37570.5
31	46131.0
32	50358.0
33	54889.5
34	64544.5
35	74465.0
36	79262.0
37	83217.5
38	89317.5
39	95087.5
40	98713.0
41	103096.0
42	109669.0
43	113999.0
44	116139.0
45	119108.5
46	123978.0
47	127867.5
48	129857.0
49	130724.0
50	130238.0
51	126870.0
52	124855.0
53	121127.5
54	115159.5
55	107532.5
56	102146.0
57	97536.5
58	85407.0
59	69580.5
60	61274.0
61	55332.5
62	42781.0
63	31044.0
64	25917.0
65	21433.5
66	14147.0
67	9678.0
68	8012.0
69	6521.5
70	4090.5
71	2540.5
72	1931.0
73	1695.5
74	1205.5
75	736.5
76	522.0
77	395.0
78	207.5
79	120.0
80	93.0
81	69.5
82	29.0
83	13.5
84	15.0
85	11.5
86	6.0
87	3.0
88	2.0
89	1.0
90	0.5
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0033639944941304236
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.09387523458914546
29	0.0
30	0.0
31	0.0
32	0.0
33	0.05635680187813791
34	0.0
35	3.95764058132991E-5
36	0.0
37	7.91528116265982E-5
38	0.0011081393627723746
39	3.561876523196919E-4
40	1.1872921743989729E-4
41	7.519517104526828E-4
42	0.0014247506092787676
43	7.91528116265982E-5
44	7.519517104526828E-4
45	0.001226868580212272
46	3.166112465063928E-4
47	1.978820290664955E-4
48	3.95764058132991E-5
49	0.0
50	1.583056232531964E-4
51	0.0010289865511457765
52	2.3745843487979457E-4
53	4.353404639462901E-4
54	0.003205688870877227
55	0.0017809382615984592
56	5.540696813861873E-4
57	4.353404639462901E-4
58	7.91528116265982E-4
59	6.332224930127856E-4
60	0.0028890776243708344
61	0.002770348406930937
62	0.0022954315371713475
63	0.0013851742034654685
64	0.002018396696478254
65	0.0023350079429846466
66	0.0031265360592506288
67	0.0026516191894910396
68	0.0038784877697033114
69	0.0036014529290102175
70	0.00395764058132991
71	0.002809924812744236
72	9.498337395191783E-4
73	3.561876523196919E-4
74	0.002453737160424544
75	0.001583056232531964
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	2526758.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.15211833904524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.05130936557748	48.51054216206801
2	12.003112308951195	14.920377133255705
3	4.384726171559683	8.175600596972584
4	1.9748611062247652	4.909672047090376
5	1.0825445886631722	3.364121969094326
6	0.6295123328208837	2.34753150032232
7	0.4211170144455276	1.8321320161482697
8	0.28159381947465495	1.4001321913225997
9	0.20275586964960818	1.1341536123958629
>10	0.9079641903167923	9.604788643843463
>50	0.04389779671240519	1.8385979139813182
>100	0.01615432944563003	1.8096275616632402
>500	4.5110615820275255E-4	0.1527226518419299
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.95764058132991E-5
2	0.0	0.0	0.0	0.0	3.95764058132991E-5
3	0.0	0.0	0.0	0.0	3.95764058132991E-5
4	0.0	0.0	0.0	0.0	3.95764058132991E-5
5	0.0	0.0	0.0	0.0	3.95764058132991E-5
6	0.0	0.0	0.0	0.0	3.95764058132991E-5
7	0.0	0.0	0.0	0.0	3.95764058132991E-5
8	0.0	0.0	0.0	0.0	3.95764058132991E-5
9	0.0	0.0	0.0	0.0	3.95764058132991E-5
10	0.0	0.0	0.0	0.0	3.95764058132991E-5
11	0.0	0.0	0.0	3.95764058132991E-5	3.95764058132991E-5
12	0.0	0.0	0.0	3.95764058132991E-5	1.583056232531964E-4
13	0.0	0.0	0.0	3.95764058132991E-5	1.583056232531964E-4
14	0.0	0.0	0.0	3.95764058132991E-5	1.583056232531964E-4
15	0.0	0.0	0.0	3.95764058132991E-5	1.978820290664955E-4
16	0.0	0.0	0.0	3.95764058132991E-5	2.3745843487979457E-4
17	0.0	0.0	0.0	3.95764058132991E-5	2.3745843487979457E-4
18	0.0	0.0	0.0	3.95764058132991E-5	4.3534046394629007E-4
19	0.0	0.0	0.0	3.95764058132991E-5	4.3534046394629007E-4
20	0.0	0.0	0.0	7.91528116265982E-5	4.3534046394629007E-4
21	0.0	0.0	0.0	7.91528116265982E-5	4.7491686975958914E-4
22	0.0	0.0	0.0	7.91528116265982E-5	4.7491686975958914E-4
23	0.0	0.0	0.0	7.91528116265982E-5	4.7491686975958914E-4
24	0.0	0.0	0.0	1.583056232531964E-4	4.7491686975958914E-4
25	0.0	0.0	0.0	1.583056232531964E-4	4.7491686975958914E-4
26	0.0	0.0	0.0	2.3745843487979457E-4	5.144932755728883E-4
27	0.0	0.0	0.0	3.166112465063928E-4	5.144932755728883E-4
28	0.0	0.0	0.0	7.91528116265982E-4	5.540696813861874E-4
29	0.0	0.0	0.0	0.0014643270150920666	5.540696813861874E-4
30	0.0	0.0	0.0	0.002889077624370834	5.540696813861874E-4
31	0.0	0.0	0.0	0.007559093510340127	5.540696813861874E-4
32	0.0	0.0	0.0	0.011991650961429626	5.540696813861874E-4
33	0.0	0.0	0.0	0.016345055600892527	5.540696813861874E-4
34	0.0	0.0	0.0	0.02152956476243471	5.540696813861874E-4
35	0.0	3.95764058132991E-5	0.0	0.02802009531581576	5.540696813861874E-4
36	0.0	3.95764058132991E-5	0.0	0.034471049463383516	5.540696813861874E-4
37	0.0	3.95764058132991E-5	0.0	0.045196255438787566	5.540696813861874E-4
38	0.0	3.95764058132991E-5	0.0	0.05928545590832205	5.936460871994864E-4
39	0.0	3.95764058132991E-5	0.0	0.07729272055337313	5.936460871994864E-4
40	0.0	3.95764058132991E-5	0.0	0.09537913801005082	5.936460871994864E-4
41	0.0	3.95764058132991E-5	0.0	0.11362386108998171	5.936460871994864E-4
42	0.0	3.95764058132991E-5	0.0	0.13396613367801743	5.936460871994864E-4
43	0.0	3.95764058132991E-5	0.0	0.1534773017439739	6.332224930127856E-4
44	0.0	3.95764058132991E-5	0.0	0.17445279682502243	6.332224930127856E-4
45	0.0	3.95764058132991E-5	0.0	0.19475549300724485	6.332224930127856E-4
46	0.0	3.95764058132991E-5	0.0	0.21711616229175884	6.332224930127856E-4
47	0.0	3.95764058132991E-5	0.0	0.24280124966458996	6.332224930127856E-4
48	0.0	3.95764058132991E-5	0.0	0.26959447640019346	6.332224930127856E-4
49	0.0	3.95764058132991E-5	0.0	0.29615024470091716	6.332224930127856E-4
50	0.0	3.95764058132991E-5	0.0	0.32559509062601166	6.332224930127856E-4
51	0.0	3.95764058132991E-5	0.0	0.354881630927853	6.332224930127856E-4
52	0.0	3.95764058132991E-5	0.0	0.3821893509390294	6.727988988260847E-4
53	0.0	3.95764058132991E-5	0.0	0.4130193710675894	6.727988988260847E-4
54	0.0	3.95764058132991E-5	0.0	0.44507625977636167	7.123753046393837E-4
55	0.0	3.95764058132991E-5	0.0	0.47938900361649195	7.123753046393837E-4
56	0.0	3.95764058132991E-5	0.0	0.5148098868193947	7.123753046393837E-4
57	0.0	3.95764058132991E-5	0.0	0.5501516172106707	7.123753046393837E-4
58	0.0	3.95764058132991E-5	0.0	0.5906382803576757	7.123753046393837E-4
59	0.0	3.95764058132991E-5	0.0	0.6307291794465477	7.123753046393837E-4
60	0.0	3.95764058132991E-5	0.0	0.6736695797539772	7.123753046393837E-4
61	0.0	3.95764058132991E-5	0.0	0.717836848641619	7.123753046393837E-4
62	0.0	3.95764058132991E-5	0.0	0.7629935276745933	7.123753046393837E-4
63	0.0	3.95764058132991E-5	0.0	0.8108414023028719	7.123753046393837E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2820	0.0	36.08638	1
TCAACGC	3495	0.0	27.535505	4
ATCAACG	3520	0.0	27.24195	3
CAACGCA	3540	0.0	27.088037	5
TGACGCG	65	8.6696586E-4	26.533384	10
GGTATCA	1455	0.0	26.316635	1
AACGCAG	3830	0.0	25.397228	6
TATCAAC	3875	0.0	25.280325	2
ACGCAGA	4325	0.0	21.772713	7
GTACATG	6230	0.0	20.376528	1
CGCAGAG	4550	0.0	20.089563	8
TCGCAAT	340	0.0	19.276867	49
TACATGG	6305	0.0	18.928972	2
ACATGGG	6360	0.0	18.168694	3
AGAGTAC	4705	0.0	18.034807	11
CGTGCCG	285	3.2923708E-10	16.944126	10
GCACCGT	480	0.0	16.528088	6
CAGAGTA	5240	0.0	16.522602	10
GCAGAGT	5475	0.0	16.128422	9
GTATAGG	565	0.0	15.874326	1
>>END_MODULE