FastQCFastQC Report
Mon 6 Feb 2017
SRR3203955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526758
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40500.040.3984261
GGTATCA17000.032.148081
TCAACGC53300.029.256694
CAACGCA53500.029.0674115
ATCAACG54450.028.965893
TATCAAC55950.028.615872
AACGCAG57100.027.4145076
CGCTACG14100.024.09668528
ACGCAGA64500.023.9901757
TATCTCG10850.023.97779836
TACGCTA13550.023.80839731
CGCTATC10950.023.75882333
GTACATG59200.023.6562611
CGCAGAG66350.023.1671668
ACGCTAT11900.023.02855332
CTCGTAT14150.022.2959839
TACATGG59950.022.094122
GCTACGC15400.022.0625529
TATGCCG14250.022.00438343
TCGTATG14700.021.93257940
Produced by FastQC (version 0.11.5)