FastQCFastQC Report
Mon 6 Feb 2017
SRR3203954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482601
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8200.038.6970331
TCAACGC10550.027.788824
GGTATCA3750.027.5926651
TATCAAC11150.027.5307982
ATCAACG10800.027.4649223
CAACGCA10800.026.8262025
TGCGATA658.6632214E-426.53140813
GCGATAC658.6632214E-426.53140814
AACGCAG10850.026.3846916
TAATACC700.001334792124.6363094
TGCGTAT851.4442089E-424.3464710
GCACCGT1151.6981685E-623.9936226
GTACATG14000.021.9263151
AGTATAC800.002901493321.5567683
GTATAGC1605.7170837E-821.5567681
CTCCTAA1455.2933683E-721.40811
TACATGG14100.021.0369592
TTAATAC1154.6049514E-520.994423
ACGCAGA13150.020.9830157
CTCTATA1356.825525E-620.4390132
Produced by FastQC (version 0.11.5)