##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203954_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482601
Sequences flagged as poor quality	0
Sequence length	75
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.32661971276479	35.0	27.0	35.0	15.0	35.0
2	30.168143041560214	35.0	27.0	35.0	15.0	35.0
3	30.158172071752855	35.0	27.0	35.0	15.0	35.0
4	30.323664890872585	35.0	30.0	35.0	15.0	35.0
5	30.485521165517685	35.0	31.0	35.0	15.0	35.0
6	33.61477286619796	39.0	30.0	40.0	16.0	40.0
7	33.47485396839211	39.0	30.0	40.0	16.0	40.0
8	33.39288149009223	39.0	30.0	40.0	16.0	40.0
9	33.292144027882244	39.0	27.0	40.0	16.0	40.0
10	33.44034098561752	39.0	28.0	40.0	16.0	40.0
11	33.43429043868537	39.0	30.0	40.0	16.0	40.0
12	33.045366669360405	39.0	27.0	40.0	16.0	40.0
13	33.067683241435475	39.0	27.0	40.0	16.0	40.0
14	33.06335046964262	39.0	27.0	40.0	16.0	40.0
15	33.14208631975483	39.0	27.0	40.0	16.0	40.0
16	33.29855304899907	39.0	27.0	40.0	16.0	40.0
17	33.3330266617765	39.0	30.0	40.0	16.0	40.0
18	33.39992250326875	39.0	30.0	40.0	16.0	40.0
19	33.454746260368296	39.0	30.0	40.0	16.0	40.0
20	33.447371638268464	39.0	30.0	40.0	16.0	40.0
21	33.47941674385258	39.0	30.0	40.0	16.0	40.0
22	33.54133124465138	39.0	31.0	40.0	16.0	40.0
23	33.57276300712183	39.0	31.0	40.0	16.0	40.0
24	33.60380314172578	39.0	31.0	40.0	16.0	40.0
25	33.62995518036639	39.0	31.0	40.0	16.0	40.0
26	33.65171642827097	39.0	31.0	40.0	16.0	40.0
27	33.656453260561	39.0	31.0	40.0	16.0	40.0
28	33.531671090611084	39.0	31.0	40.0	16.0	40.0
29	33.63513751525588	39.0	31.0	40.0	16.0	40.0
30	33.6216667599114	39.0	31.0	40.0	16.0	40.0
31	33.59992623305795	39.0	31.0	40.0	16.0	40.0
32	33.55450983317482	39.0	31.0	40.0	16.0	40.0
33	33.41693655835773	39.0	31.0	40.0	16.0	40.0
34	33.437999506838985	39.0	31.0	40.0	16.0	40.0
35	33.399771239595445	39.0	31.0	40.0	16.0	40.0
36	33.41637501787191	39.0	31.0	40.0	16.0	40.0
37	33.36989355596031	39.0	31.0	40.0	16.0	40.0
38	33.35373113607307	39.0	31.0	40.0	16.0	40.0
39	33.28117844762029	39.0	31.0	40.0	16.0	40.0
40	33.2016158275677	39.0	30.0	40.0	16.0	40.0
41	33.14026286725473	39.0	30.0	40.0	16.0	40.0
42	33.12254015221684	39.0	30.0	40.0	16.0	40.0
43	33.03242430081993	39.0	30.0	40.0	16.0	40.0
44	32.89119997679242	38.0	30.0	40.0	16.0	40.0
45	32.89159160465892	38.0	30.0	40.0	16.0	40.0
46	32.8307877522011	38.0	30.0	40.0	16.0	40.0
47	32.736349489536906	38.0	30.0	40.0	16.0	40.0
48	32.7622715245099	38.0	30.0	40.0	16.0	40.0
49	32.69959448902924	38.0	30.0	40.0	16.0	40.0
50	32.58773603867377	38.0	27.0	40.0	16.0	40.0
51	32.61228012374612	38.0	28.0	40.0	16.0	40.0
52	32.61965889005617	38.0	30.0	40.0	16.0	40.0
53	32.547054398975554	38.0	28.0	40.0	16.0	40.0
54	32.576190268979964	38.0	30.0	40.0	16.0	40.0
55	32.53131054432129	38.0	30.0	40.0	16.0	40.0
56	32.4845742134807	38.0	28.0	40.0	16.0	40.0
57	32.361213507638816	38.0	27.0	40.0	16.0	40.0
58	32.36596691676976	38.0	27.0	40.0	16.0	40.0
59	32.276151520614334	38.0	27.0	40.0	16.0	40.0
60	32.2134475477672	38.0	27.0	40.0	16.0	40.0
61	32.179172857080694	38.0	27.0	40.0	16.0	40.0
62	32.12041210026502	38.0	27.0	40.0	16.0	40.0
63	32.079929382657724	38.0	27.0	40.0	16.0	40.0
64	32.04667002347695	38.0	27.0	39.0	16.0	40.0
65	31.954716214844147	38.0	27.0	39.0	16.0	40.0
66	31.906438237798927	38.0	27.0	39.0	16.0	40.0
67	31.866280840694486	38.0	27.0	39.0	16.0	40.0
68	31.826193895163915	38.0	27.0	39.0	16.0	40.0
69	31.786635336437346	38.0	27.0	39.0	16.0	40.0
70	31.67013951483731	38.0	27.0	39.0	16.0	40.0
71	31.544922202813503	38.0	27.0	39.0	15.0	40.0
72	31.502309361149273	38.0	27.0	39.0	15.0	40.0
73	31.42858386120211	38.0	27.0	39.0	15.0	40.0
74	31.34417251518335	38.0	27.0	39.0	15.0	40.0
75	31.230326916023795	38.0	27.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6876.0
3	958.0
4	1099.0
5	1074.0
6	1081.0
7	1013.0
8	1029.0
9	1109.0
10	1173.0
11	1275.0
12	1351.0
13	1416.0
14	1577.0
15	1798.0
16	1893.0
17	1991.0
18	2553.0
19	4702.0
20	7786.0
21	9022.0
22	9135.0
23	9391.0
24	9739.0
25	9932.0
26	10668.0
27	11067.0
28	12069.0
29	12952.0
30	13666.0
31	14983.0
32	16406.0
33	18193.0
34	20021.0
35	23003.0
36	27972.0
37	38421.0
38	66699.0
39	107508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.852984326174706	15.475933922932192	11.62875993601167	27.042321814881433
2	16.225411882693987	20.420388685477235	36.80949687215733	26.544702559671446
3	19.937588193973905	23.875209541629626	27.062936048619875	29.124266215776593
4	13.952312572912199	16.07228331478799	34.20092374445971	35.7744803678401
5	15.481526146858377	36.9537153880742	31.50697988607566	16.057778578991755
6	34.28857379077126	34.949782532568314	16.001002898875054	14.760640777785374
7	30.327744865841556	31.22579522214003	19.441526229742582	19.004933682275833
8	27.396544971933334	34.9037817990431	18.056945592736028	19.642727636287532
9	27.889084357471283	16.211114357409123	16.54803864890458	39.35176263621501
10	15.100880437462832	29.178969790779547	31.518376464201275	24.201773307556344
11	36.557114469302796	23.039943970277722	20.94877548948303	19.454166070936445
12	25.2415556536352	24.62096017206761	27.712333791268563	22.42515038302863
13	29.573705814948582	21.606254442075336	23.30102921461	25.51901052836608
14	24.009274742489136	20.49187631190155	24.78299879196272	30.715850153646596
15	24.953118621801444	27.616602535013396	22.354284388138442	25.07599445504671
16	25.437784007907155	27.073710995211364	23.01777244556062	24.470732551320864
17	24.501192496492962	26.66820002445084	24.07081626436746	24.75979121468874
18	24.165925889088502	26.480674511656627	25.120959136015053	24.23244046323982
19	25.310763964434386	25.770149668152364	25.134013398231662	23.78507296918158
20	25.376656907051583	25.47736121557974	25.523983580639083	23.621998296729597
21	25.762690089742872	25.15742818601702	25.153905607323644	23.92597611691646
22	25.472388163306746	25.053822930329613	25.129247556470048	24.344541349893596
23	25.542839737174187	24.99207419793991	24.89219873145725	24.57288733342865
24	24.451876394785753	25.472595373818123	25.21544712920197	24.86008110219415
25	25.34225996216336	24.912090940549234	25.095265032604573	24.650384064682836
26	24.62531159280648	25.806618718154333	25.320088437446252	24.247981251592932
27	24.74383600531288	25.67234630678345	25.025642300782636	24.558175387121036
28	25.258900515415807	25.4789633608844	24.944465761664265	24.31767036203553
29	25.175455500506626	25.68125635877257	24.90090157293499	24.24238656778581
30	25.4415137971119	25.549470473538182	24.848270103045788	24.16074562630413
31	25.228915812441333	25.09940924283207	24.86008110219415	24.811593842532446
32	24.563770070928157	25.66882372809008	25.09940924283207	24.667996958149693
33	24.910480100353517	25.376792212235248	25.095014462103073	24.61771322530816
34	25.20902360334935	25.395927484609437	24.993110250496787	24.401938661544424
35	25.212338971531352	25.193690025507614	25.091949664422575	24.502021338538462
36	25.137121555902286	25.379557854210827	24.947316727482953	24.53600386240393
37	25.026885563850882	24.90939720390136	25.239276338010075	24.824440894237682
38	24.691565236490824	25.44685224555427	25.162143233084265	24.69943928487064
39	25.18581602607537	25.26911465164805	25.142094608175285	24.402974714101298
40	25.072886297376094	25.04139029964712	25.69265293689818	24.193070466078602
41	24.7469441629835	25.003885197088277	25.544911842287938	24.704258797640286
42	24.693844566284984	25.06185272214141	25.791445468070734	24.452857243502873
43	24.34682066551872	25.197212604200985	25.53786668490119	24.918100045379102
44	24.63639584831299	25.4480427850037	25.426078379772854	24.489482986910456
45	24.686799365103045	25.66898329458473	25.206693769970034	24.43752357034219
46	24.765385898495857	25.60168752240464	24.70011458741279	24.932811991686716
47	24.371478716372323	25.781131825255233	25.01776830135039	24.829621157022054
48	24.20881846494309	25.918926815319487	25.061489719250478	24.810765000486946
49	24.750881162699624	25.490001056773608	24.78610694963334	24.97301083089343
50	24.828480788397822	25.610703710534004	24.96337539033442	24.597440110733757
51	24.527817090379383	25.665821934981214	24.66001794450465	25.146343030134748
52	24.592416079569002	25.874015748031496	24.528802320762537	25.004765851636968
53	24.662038955656858	25.650849564857026	24.731454620803976	24.955656858682136
54	24.50682569811102	25.854766384576493	24.706374795892145	24.932033121420343
55	24.57909667152238	25.356148970250292	24.759580018773583	25.30517433945374
56	24.66659759635309	25.82905097389142	24.7563199336925	24.748031496062993
57	24.82392213825557	25.51414321206633	24.371372505952145	25.290562143725953
58	24.463167142907466	25.418618770449168	24.817913008522606	25.300301078120757
59	25.213012847078325	25.484251968503933	24.35060091172814	24.952134272689598
60	24.6570091153415	25.661582327136944	24.554438211909417	25.126970345612143
61	24.70347377058692	25.353303438958285	24.812676651719478	25.130546138735323
62	24.969850433283465	25.481046179604387	24.490358355056216	25.058745032055928
63	24.534756743203957	25.587187653466557	24.45249348835571	25.425562114973776
64	24.68736725411577	25.339259627638082	24.44575679399911	25.527616324247038
65	24.86805706648432	25.41862224018069	24.47248728229675	25.240833411038246
66	24.584480790071883	26.003079224764758	24.44108755069013	24.971352434473225
67	24.67166746099173	25.473383203133093	24.313392320603413	25.541557015271763
68	24.626695566763786	25.787948991052296	24.730304901550408	24.85505054063351
69	24.784442119004943	25.502657562916376	24.337474227338188	25.375426090740493
70	24.489411082100375	25.24659123875834	24.913589456670397	25.35040822247089
71	24.7540344973352	25.407179623198257	24.6817160807977	25.157069798668847
72	24.232120100705558	25.619204508956784	24.63204135973228	25.516634030605374
73	24.51906340654787	25.396187318690426	24.684210526315788	25.400538748445918
74	24.388252626954802	25.575795753357383	24.58552030385853	25.45043131582928
75	24.675607239211427	25.31817635528001	24.65177768476193	25.35443872074663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96.0
1	118.0
2	172.5
3	204.5
4	204.0
5	181.0
6	168.0
7	193.0
8	208.0
9	245.5
10	351.0
11	414.5
12	410.0
13	438.0
14	474.5
15	538.5
16	594.0
17	690.5
18	802.5
19	956.0
20	1094.0
21	1161.5
22	1446.5
23	1854.0
24	2044.0
25	2253.5
26	2688.5
27	3148.0
28	3382.0
29	3646.0
30	4235.0
31	4978.5
32	5397.0
33	5645.0
34	6404.5
35	7337.0
36	7758.0
37	8338.0
38	9146.5
39	9927.5
40	10480.0
41	11272.5
42	12711.5
43	14317.5
44	15277.0
45	16197.5
46	18434.0
47	21130.0
48	22510.0
49	24032.5
50	26727.0
51	28605.0
52	29311.0
53	29642.5
54	29919.0
55	29437.0
56	29010.0
57	28157.5
58	25531.0
59	21558.0
60	19359.0
61	18020.0
62	14369.0
63	10646.0
64	9235.0
65	7670.5
66	5166.0
67	3674.5
68	3123.0
69	2626.0
70	1745.5
71	1054.5
72	747.0
73	659.5
74	460.5
75	289.0
76	229.0
77	168.5
78	86.0
79	45.5
80	27.0
81	21.0
82	10.5
83	4.0
84	2.0
85	2.0
86	1.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0026937366478726734
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.09656009830066659
29	0.0
30	0.0
31	0.0
32	0.0
33	0.06340641648069523
34	0.0
35	0.0
36	0.0
37	0.0
38	6.216315341244631E-4
39	0.0
40	0.0
41	0.0
42	6.216315341244631E-4
43	0.0
44	4.1442102274964206E-4
45	6.216315341244631E-4
46	0.0
47	0.0
48	0.0
49	0.0
50	4.1442102274964206E-4
51	4.1442102274964206E-4
52	2.0721051137482103E-4
53	2.0721051137482103E-4
54	0.0026937366478726734
55	0.0016576840909985683
56	2.0721051137482103E-4
57	4.1442102274964206E-4
58	4.1442102274964206E-4
59	2.0721051137482103E-4
60	0.0016576840909985683
61	0.0026937366478726734
62	0.001450473579623747
63	8.288420454992841E-4
64	0.0012432630682489261
65	0.0012432630682489261
66	0.0024865261364978523
67	0.002279315625123031
68	0.0039369997161216
69	0.0033153681819971365
70	0.004351420738871242
71	0.0026937366478726734
72	0.0012432630682489261
73	2.0721051137482103E-4
74	0.0016576840909985683
75	0.001450473579623747
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	482601.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.82223515005639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.89236859668134	77.9451609760336
2	5.335013402727516	9.050555227497117
3	1.3061486436318257	3.3237134217319775
4	0.5331699719027544	1.8089867492673755
5	0.2710744476158881	1.1496570269423252
6	0.1590777065594583	0.8095995979750824
7	0.10861842400964807	0.644928025208243
8	0.07558978847742709	0.5129355850540274
9	0.05532541217865201	0.4223542609432273
>10	0.2520391381092576	3.5978689144951876
>50	0.010096877132005183	0.5650412466042529
>100	0.0014775909742359664	0.16919896824752131
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.0721051137482103E-4	0.0	0.0	0.0	0.0
3	2.0721051137482103E-4	0.0	0.0	0.0	0.0
4	2.0721051137482103E-4	0.0	0.0	0.0	0.0
5	2.0721051137482103E-4	0.0	0.0	0.0	0.0
6	2.0721051137482103E-4	0.0	0.0	0.0	0.0
7	2.0721051137482103E-4	0.0	0.0	0.0	0.0
8	2.0721051137482103E-4	0.0	0.0	0.0	0.0
9	2.0721051137482103E-4	0.0	0.0	0.0	0.0
10	2.0721051137482103E-4	0.0	0.0	0.0	0.0
11	2.0721051137482103E-4	0.0	0.0	0.0	0.0
12	2.0721051137482103E-4	0.0	0.0	0.0	0.0
13	2.0721051137482103E-4	0.0	0.0	0.0	0.0
14	2.0721051137482103E-4	0.0	0.0	0.0	0.0
15	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0	0.0
16	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0	0.0
17	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0	0.0
18	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0	0.0
19	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0	0.0
20	2.0721051137482103E-4	2.0721051137482103E-4	0.0	2.0721051137482103E-4	0.0
21	2.0721051137482103E-4	2.0721051137482103E-4	0.0	4.1442102274964206E-4	0.0
22	2.0721051137482103E-4	2.0721051137482103E-4	0.0	6.216315341244631E-4	0.0
23	2.0721051137482103E-4	2.0721051137482103E-4	0.0	8.288420454992841E-4	0.0
24	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0016576840909985683	0.0
25	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0018648946023733892	0.0
26	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0024865261364978523	0.0
27	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0035225786933719572	0.0
28	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.006837946875369093	0.0
29	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.012018209659739619	0.0
30	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.021549893182981388	0.0
31	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.04496468096833616	0.0
32	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.0690011002878154	0.0
33	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.09448799318691839	0.0
34	2.0721051137482103E-4	2.0721051137482103E-4	0.0	0.12681283296139045	0.0
35	4.1442102274964206E-4	2.0721051137482103E-4	0.0	0.15250693637186827	0.0
36	4.1442102274964206E-4	2.0721051137482103E-4	0.0	0.1893904073965864	0.0
37	4.1442102274964206E-4	2.0721051137482103E-4	0.0	0.24098582472891686	0.0
38	4.1442102274964206E-4	2.0721051137482103E-4	0.0	0.31102297757360636	0.0
39	4.1442102274964206E-4	2.0721051137482103E-4	0.0	0.38893412985053905	0.0
40	4.1442102274964206E-4	2.0721051137482103E-4	0.0	0.46953901877534443	0.0
41	4.1442102274964206E-4	2.0721051137482103E-4	0.0	0.5416482767337821	0.0
42	4.1442102274964206E-4	4.1442102274964206E-4	0.0	0.6232892182154617	0.0
43	4.1442102274964206E-4	4.1442102274964206E-4	0.0	0.6962273182193986	0.0
44	4.1442102274964206E-4	4.1442102274964206E-4	0.0	0.771030312825709	0.0
45	4.1442102274964206E-4	4.1442102274964206E-4	0.0	0.8566082540235102	0.0
46	4.1442102274964206E-4	4.1442102274964206E-4	0.0	0.9457087739146831	0.0
47	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.044133766817723	0.0
48	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.140486654607015	0.0
49	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.2277222798958145	0.0
50	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.3224174835941078	0.0
51	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.4206352659857728	0.0
52	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.518853048377438	0.0
53	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.6108545154278586	0.0
54	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.7061713506602763	0.0
55	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.8023170279381933	0.0
56	4.1442102274964206E-4	4.1442102274964206E-4	0.0	1.9026069154436067	0.0
57	4.1442102274964206E-4	4.1442102274964206E-4	0.0	2.0026895924376453	0.0
58	4.1442102274964206E-4	4.1442102274964206E-4	0.0	2.1044299535226823	0.0
59	4.1442102274964206E-4	4.1442102274964206E-4	0.0	2.1997467887551	0.0
60	4.1442102274964206E-4	4.1442102274964206E-4	0.0	2.3091539387610056	0.0
61	4.1442102274964206E-4	4.1442102274964206E-4	0.0	2.415245720584914	0.0
62	4.1442102274964206E-4	4.1442102274964206E-4	0.0	2.535220606670935	0.0
63	4.1442102274964206E-4	4.1442102274964206E-4	0.0	2.6429700725858423	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	820	0.0	38.697033	1
TCAACGC	1055	0.0	27.78882	4
GGTATCA	375	0.0	27.592665	1
TATCAAC	1115	0.0	27.530798	2
ATCAACG	1080	0.0	27.464922	3
CAACGCA	1080	0.0	26.826202	5
TGCGATA	65	8.6632214E-4	26.531408	13
GCGATAC	65	8.6632214E-4	26.531408	14
AACGCAG	1085	0.0	26.384691	6
TAATACC	70	0.0013347921	24.636309	4
TGCGTAT	85	1.4442089E-4	24.34647	10
GCACCGT	115	1.6981685E-6	23.993622	6
GTACATG	1400	0.0	21.926315	1
AGTATAC	80	0.0029014933	21.556768	3
GTATAGC	160	5.7170837E-8	21.556768	1
CTCCTAA	145	5.2933683E-7	21.4081	1
TACATGG	1410	0.0	21.036959	2
TTAATAC	115	4.6049514E-5	20.99442	3
ACGCAGA	1315	0.0	20.983015	7
CTCTATA	135	6.825525E-6	20.439013	2
>>END_MODULE