FastQCFastQC Report
Mon 6 Feb 2017
SRR3203954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482601
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA6990.1448401474509999TruSeq Adapter, Index 1 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.13282193779126028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16550.044.778631
GGTATCA5800.038.8619881
AATCTCG1056.0754246E-1033.02219826
TATCAAC22300.032.9126132
ATCAACG23100.031.1816643
TCAACGC23200.030.8905014
AGGCGTA450.004651695530.62385244
CAACGCA23700.030.2199945
AACGCAG24100.029.8599346
GTGATAC605.749203E-428.4595361
GTATAAC500.00812318527.3211561
GTACTAA756.5969434E-527.3211541
ACGCAGA26350.026.9107697
ATCGTAT1554.9112714E-1126.8551736
CCAAACG658.400146E-426.67177424
CGCAGAG26850.026.4096368
AGAGTAC26600.026.02512411
GAGTACT22400.025.5167412
AGTACTT22350.025.1170313
CAGAGTA27800.024.89399510
Produced by FastQC (version 0.11.5)