FastQCFastQC Report
Mon 6 Feb 2017
SRR3203953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539471
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12650.035.9959681
GGTATCA7450.028.7081931
CAACGCA16850.025.9983655
TCAACGC17200.025.4693264
TATCAAC17700.024.7498552
ATCAACG17750.024.4858073
AACGCAG17750.023.9028116
TAGATCG750.001995380522.995935
AATCGCA1352.6650196E-722.9959347
GTACATG37950.022.8156261
TACATGG38500.022.0402552
ACATGGG38950.020.7229063
GCGATAC1851.1794327E-820.50988214
TCCGTTA1451.26541745E-519.03420465
ACGCAGA21950.019.0148567
GTATATG4000.018.9728741
GTATTAT2608.913048E-1118.574841
TGCGATA2255.5497367E-918.39674213
GTATAGT2458.039933E-1018.3040711
CCGTTAT1753.209676E-617.74259666
Produced by FastQC (version 0.11.5)