##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3203953_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1539471 Sequences flagged as poor quality 0 Sequence length 75 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60040234600067 35.0 31.0 35.0 27.0 35.0 2 31.508510390907006 35.0 31.0 35.0 15.0 35.0 3 31.4730988761724 35.0 31.0 35.0 15.0 35.0 4 31.765127111845562 35.0 31.0 35.0 27.0 35.0 5 31.817364536259532 35.0 31.0 35.0 27.0 35.0 6 35.33041869577277 39.0 35.0 40.0 16.0 40.0 7 35.213621432297195 39.0 34.0 40.0 16.0 40.0 8 35.172090932534616 39.0 34.0 40.0 16.0 40.0 9 35.183620217594225 39.0 34.0 40.0 16.0 40.0 10 35.26896122109478 39.0 34.0 40.0 16.0 40.0 11 35.325320840730356 39.0 35.0 40.0 16.0 40.0 12 34.89911924290877 39.0 34.0 40.0 16.0 40.0 13 34.97067109416157 39.0 34.0 40.0 16.0 40.0 14 34.937511651729714 39.0 34.0 40.0 16.0 40.0 15 34.98714558442478 39.0 34.0 40.0 16.0 40.0 16 35.140359902849745 39.0 34.0 40.0 16.0 40.0 17 35.17479705691111 39.0 34.0 40.0 16.0 40.0 18 35.230541530174975 39.0 34.0 40.0 16.0 40.0 19 35.26774846684348 39.0 34.0 40.0 16.0 40.0 20 35.216243112082005 39.0 34.0 40.0 16.0 40.0 21 35.23115992441559 39.0 34.0 40.0 16.0 40.0 22 35.29365217012857 39.0 34.0 40.0 16.0 40.0 23 35.299567188988945 39.0 35.0 40.0 16.0 40.0 24 35.32343902548343 39.0 35.0 40.0 16.0 40.0 25 35.33105397893173 39.0 35.0 40.0 16.0 40.0 26 35.328633017445604 39.0 35.0 40.0 16.0 40.0 27 35.32279269957018 39.0 35.0 40.0 16.0 40.0 28 35.197994635819704 39.0 34.0 40.0 16.0 40.0 29 35.28162920899452 39.0 35.0 40.0 16.0 40.0 30 35.30097936239137 39.0 35.0 40.0 16.0 40.0 31 35.29103828522915 39.0 35.0 40.0 16.0 40.0 32 35.23217845610603 39.0 34.0 40.0 16.0 40.0 33 35.11758714519468 39.0 34.0 40.0 16.0 40.0 34 35.16133853771848 39.0 34.0 40.0 16.0 40.0 35 35.154910355570195 39.0 34.0 40.0 16.0 40.0 36 35.17255212991995 39.0 34.0 40.0 16.0 40.0 37 35.13702044403565 39.0 34.0 40.0 16.0 40.0 38 35.15360925928452 39.0 34.0 40.0 16.0 40.0 39 35.1201282778305 39.0 34.0 40.0 16.0 40.0 40 35.03393308480641 39.0 34.0 40.0 16.0 40.0 41 35.00607871145348 39.0 34.0 40.0 16.0 40.0 42 35.02239145784493 39.0 34.0 40.0 16.0 40.0 43 34.945728760074076 39.0 34.0 40.0 16.0 40.0 44 34.81900600920706 39.0 34.0 40.0 16.0 40.0 45 34.841892442274 39.0 34.0 40.0 16.0 40.0 46 34.795954584399446 39.0 34.0 40.0 16.0 40.0 47 34.72725696034547 39.0 34.0 40.0 16.0 40.0 48 34.76255220137307 39.0 34.0 40.0 16.0 40.0 49 34.72118474462981 39.0 34.0 40.0 16.0 40.0 50 34.609569131214556 39.0 34.0 40.0 16.0 40.0 51 34.657384257319556 39.0 34.0 40.0 16.0 40.0 52 34.686617675812016 39.0 34.0 40.0 16.0 40.0 53 34.63422955028059 39.0 34.0 40.0 16.0 40.0 54 34.65912446548197 39.0 34.0 40.0 16.0 40.0 55 34.65417276454055 39.0 34.0 40.0 16.0 40.0 56 34.60432447249737 39.0 34.0 40.0 16.0 40.0 57 34.48491527284372 39.0 34.0 40.0 16.0 40.0 58 34.51906466571959 39.0 34.0 40.0 16.0 40.0 59 34.463293559930655 39.0 34.0 40.0 16.0 40.0 60 34.42503691203017 39.0 34.0 40.0 16.0 40.0 61 34.39134611824451 39.0 34.0 40.0 16.0 40.0 62 34.35377282196287 39.0 34.0 40.0 16.0 40.0 63 34.31225401452837 39.0 34.0 40.0 16.0 40.0 64 34.305530925882984 39.0 34.0 40.0 16.0 40.0 65 34.21718564363992 39.0 34.0 40.0 16.0 40.0 66 34.174248816638965 39.0 34.0 40.0 16.0 40.0 67 34.1721097701743 39.0 34.0 40.0 16.0 40.0 68 34.13682362317965 39.0 34.0 40.0 16.0 40.0 69 34.10668859627755 39.0 34.0 40.0 16.0 40.0 70 33.99762320953106 39.0 34.0 40.0 16.0 40.0 71 33.89412467009772 39.0 34.0 40.0 16.0 40.0 72 33.87581383475233 39.0 34.0 40.0 16.0 40.0 73 33.8181998881434 39.0 34.0 40.0 16.0 40.0 74 33.77311427107104 39.0 34.0 40.0 16.0 40.0 75 33.674858441633525 39.0 34.0 40.0 16.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3416.0 3 882.0 4 1054.0 5 1009.0 6 1001.0 7 1095.0 8 1181.0 9 1288.0 10 1369.0 11 1462.0 12 1692.0 13 1896.0 14 2132.0 15 2524.0 16 2776.0 17 3237.0 18 4036.0 19 7219.0 20 13290.0 21 17690.0 22 21012.0 23 23238.0 24 25700.0 25 27628.0 26 29908.0 27 32110.0 28 34865.0 29 37565.0 30 41471.0 31 45415.0 32 50581.0 33 57123.0 34 65277.0 35 77412.0 36 98045.0 37 136431.0 38 239424.0 39 426017.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.963531009036046 15.253639428604837 11.7697919305699 27.013037631789217 2 16.364517421893623 18.681157358599155 37.97239441340564 26.981930806101577 3 19.94912538138101 22.511888824148034 27.1136643691242 30.42532142534676 4 13.493791049003196 15.037438184934954 34.883736036599586 36.585034729462265 5 15.443551713543158 36.30851117039555 32.768009270717016 15.479927845344276 6 35.32388723139312 33.98472592208622 16.37737898278045 14.314007863740208 7 31.088146512665716 29.92865731150506 19.839802113843003 19.14339406198623 8 27.847033169186037 34.14504073152401 18.648808584247444 19.359117515042502 9 27.70334744857162 15.03470997504987 17.001944174330013 40.259998402048495 10 15.164949518373518 28.215536375807016 32.37774534239359 24.241768763425878 11 37.69164862475486 21.487575927055463 21.528758904844587 19.292016543345085 12 25.146300255087624 23.033756400737655 28.672381616802134 23.147561727372583 13 29.815241729139423 20.170889870611397 24.008117073981907 26.00575132626727 14 23.60083431256581 19.01088101042501 25.27887826402706 32.109406412982125 15 25.347278383288806 26.856757938278797 22.090380396902574 25.705583281529826 16 25.75722439721177 25.97885897168573 23.197578908599123 25.06633772250338 17 24.690916153556845 25.825008493188236 24.53592764777985 24.94814770547507 18 24.732132011580603 25.760472266122587 24.92369131994042 24.583704402356393 19 25.61301901757162 25.254584204574172 24.757790175976034 24.374606601878178 20 25.708506363549553 24.87620747646432 24.90426906385375 24.511017096132374 21 25.932284531504653 24.601372809231222 24.779485940300273 24.686856718963853 22 25.618150650450705 24.520435915973735 24.67938662046898 25.182026813106578 23 25.38443400362852 24.585847995837533 24.679321663090764 25.350396337443186 24 25.01761968884117 24.554668454293715 24.938631516930165 25.48908033993495 25 25.537083842436786 24.3878579070343 24.75785513335425 25.317203117174664 26 25.17299773753452 25.01164361004527 24.828009101827835 24.987349550592377 27 25.267445765460995 25.049448804167145 24.669383184223673 25.01372224614819 28 25.453684842235162 24.90440612815073 24.47827299698248 25.16363603263163 29 25.371052783715964 25.086669381885073 24.5346615818031 25.00761625259586 30 25.570147147948873 24.971499950307603 24.473861475792656 24.98449142595086 31 25.571641167647847 24.603581360090576 24.501078617265282 25.323698854996294 32 25.18709348860745 25.006771806679044 24.697055027343808 25.109079677369696 33 25.489430205299747 24.62069687140316 24.527824878125816 25.362048045171274 34 25.463147009226567 24.792785559686852 24.720487856844052 25.023579574242532 35 25.51322924542764 24.745886241220994 24.716850236575233 25.02403427677613 36 25.55088800099255 24.901573204786846 24.5491789701514 24.998359824069208 37 25.548192978478916 24.72992275898087 24.551939080214126 25.169945182326092 38 25.483938440432052 24.858910833205584 24.58504714664699 25.072103579715378 39 25.43211384199856 25.044901904360405 24.538882613268097 24.984101640372934 40 25.611639865200186 24.702949330547955 24.648839729049257 25.036571075202602 41 25.273034228258105 24.767921717954163 24.709004916662284 25.250039137125448 42 25.40187975858958 24.763666055410592 24.739306753762538 25.095147432237297 43 25.21099835202703 24.549405735881315 24.774678508860536 25.46491740323112 44 25.313600834579987 24.871626813870083 24.627839556415022 25.186932795134908 45 25.380898280103388 24.886436935716777 24.501495011539813 25.231169772640023 46 25.492102775964376 24.708843656724902 24.296427655061986 25.50262591224873 47 25.226426063600016 24.83836917370645 24.599519963104065 25.33568479958947 48 25.11843384755893 24.98228282905707 24.61014104232179 25.28914228106221 49 25.487051370993097 24.728022927400893 24.522367467202955 25.262558234403055 50 25.52888537828751 24.742118035887817 24.62740278434572 25.101593801478955 51 25.277891093713865 24.76134364696009 24.548020984090442 25.412744275235603 52 25.326217436718235 24.94225262818747 24.41706983729402 25.31446009780027 53 25.268535022579286 24.901913912894358 24.56660240187494 25.262948662651418 54 25.15939517205584 24.88058760059841 24.68902107491622 25.270996152429532 55 25.141837475754137 24.609565308898443 24.58130824365775 25.66728897168967 56 25.00716160677225 25.034054170978248 24.79832200792616 25.160462214323342 57 25.46568277188105 24.669884245124916 24.47676457978772 25.38766840320632 58 25.22043529718978 24.671929138512745 24.59300505244067 25.5146305118568 59 25.43331837134782 24.796730275675124 24.50299034010044 25.26696101287662 60 25.34454445299021 24.995972519591746 24.719115721204815 24.940367306213233 61 25.517518690565822 24.59236085442958 24.712081813002747 25.178038642001855 62 25.20916805678978 25.059307588773 24.648311844888244 25.083212509548975 63 25.058349026238712 24.6746388141685 24.824238377127312 25.442773782465476 64 25.26056569958277 24.637676452266053 24.64521166476879 25.45654618338239 65 25.42866459837135 24.673191151021935 24.617586226608257 25.280558023998463 66 25.310848601579174 24.795068256448065 24.56965795448271 25.32442518749005 67 25.36261868864194 24.778440289796137 24.4180464549472 25.44089456661472 68 25.227457863617524 24.917792966897622 24.629110189254817 25.225638980230038 69 25.32145432340962 24.788717755503733 24.56005872379678 25.32976919728987 70 25.174452218086387 24.62676774566549 24.779685459824996 25.419094576423127 71 25.31731003459084 24.606005489062476 24.630819949006934 25.44586452733975 72 25.044821027687703 24.949462667331836 24.598559491144925 25.407156813835535 73 25.16250859881114 24.793612293165708 24.6043247716565 25.439554336366655 74 25.286746302357955 24.864121520539786 24.516199858778624 25.332932318323635 75 25.12517571370294 24.844943212968907 24.70768515222458 25.322195921103575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 88.0 1 114.5 2 139.5 3 145.5 4 153.0 5 150.5 6 155.5 7 164.0 8 165.0 9 216.5 10 334.5 11 405.5 12 410.0 13 418.0 14 475.0 15 578.0 16 632.0 17 677.0 18 809.5 19 1058.5 20 1220.0 21 1308.0 22 1660.5 23 2178.5 24 2432.0 25 2744.0 26 3418.5 27 4123.0 28 4465.0 29 4958.0 30 5992.0 31 7182.5 32 7832.0 33 8639.5 34 10484.5 35 13100.5 36 14679.0 37 16779.0 38 21942.5 39 29244.5 40 33483.0 41 38462.0 42 49648.0 43 61545.0 44 67235.0 45 72676.0 46 82842.5 47 90652.5 48 93737.0 49 96681.0 50 101462.0 51 103483.0 52 103667.0 53 102345.0 54 98362.5 55 92098.5 56 88495.0 57 85065.5 58 75614.0 59 63341.0 60 57090.0 61 51342.0 62 40021.0 63 29773.5 64 25099.0 65 20845.5 66 13921.0 67 9680.0 68 8110.0 69 6818.5 70 4591.0 71 3003.5 72 2352.0 73 2039.0 74 1505.0 75 979.0 76 674.0 77 509.0 78 263.5 79 135.0 80 87.0 81 69.0 82 30.5 83 9.0 84 8.0 85 5.0 86 2.0 87 2.5 88 3.0 89 2.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005261547635518954 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.2991475643256677E-4 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.09444802792647604 29 0.0 30 0.0 31 0.0 32 0.0 33 0.05157615830372901 34 1.9487213464885013E-4 35 1.9487213464885013E-4 36 1.2991475643256677E-4 37 2.5982951286513355E-4 38 0.0012341901861093844 39 2.5982951286513355E-4 40 1.9487213464885013E-4 41 7.794885385954005E-4 42 0.0011692328078931008 43 2.5982951286513355E-4 44 9.094032950279674E-4 45 0.0011692328078931008 46 3.8974426929770027E-4 47 3.8974426929770027E-4 48 2.5982951286513355E-4 49 1.9487213464885013E-4 50 4.547016475139837E-4 51 0.0012341901861093844 52 4.547016475139837E-4 53 4.547016475139837E-4 54 0.0037675279365444367 55 0.002143593481137352 56 7.794885385954005E-4 57 7.145311603791172E-4 58 0.0011042754296768176 59 9.094032950279674E-4 60 0.0030529967761653187 61 0.0037675279365444367 62 0.002533337750435052 63 0.0018188065900559347 64 0.0020786361029210684 65 0.002533337750435052 66 0.0036376131801118695 67 0.002858124641516469 68 0.00422222958405842 69 0.003962400071193286 70 0.005261547635518954 71 0.0029880393979490358 72 9.743606732442508E-4 73 9.094032950279674E-4 74 0.002858124641516469 75 0.0022735082375699186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 1539471.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.60810461368696 #Duplication Level Percentage of deduplicated Percentage of total 1 82.5867362718271 58.312929143841444 2 10.461713957720159 14.773635871303481 3 3.2466866141493695 6.877271642991474 4 1.3546112356807827 3.8258612735929787 5 0.7092339824808668 2.5038833615295437 6 0.4292285948569316 1.818421051730645 7 0.2707513366210678 1.3382067090305152 8 0.18940552771092323 1.069885225201876 9 0.1401109798551885 0.8903673650827202 >10 0.5829193902190802 6.897083304019339 >50 0.023490267879986575 1.0973313628364438 >100 0.005111840998675003 0.5951236888395709 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 6.495737821628339E-5 0.0 0.0 0.0 4 0.0 6.495737821628339E-5 0.0 0.0 0.0 5 0.0 2.5982951286513355E-4 0.0 0.0 0.0 6 0.0 2.5982951286513355E-4 0.0 0.0 0.0 7 0.0 2.5982951286513355E-4 0.0 0.0 0.0 8 0.0 2.5982951286513355E-4 0.0 0.0 0.0 9 0.0 2.5982951286513355E-4 0.0 0.0 0.0 10 0.0 2.5982951286513355E-4 0.0 0.0 0.0 11 0.0 2.5982951286513355E-4 0.0 6.495737821628339E-5 0.0 12 0.0 2.5982951286513355E-4 0.0 6.495737821628339E-5 0.0 13 0.0 2.5982951286513355E-4 0.0 6.495737821628339E-5 0.0 14 0.0 2.5982951286513355E-4 0.0 6.495737821628339E-5 0.0 15 0.0 2.5982951286513355E-4 0.0 6.495737821628339E-5 6.495737821628339E-5 16 0.0 2.5982951286513355E-4 0.0 6.495737821628339E-5 6.495737821628339E-5 17 0.0 2.5982951286513355E-4 0.0 6.495737821628339E-5 6.495737821628339E-5 18 0.0 3.247868910814169E-4 0.0 6.495737821628339E-5 6.495737821628339E-5 19 0.0 3.247868910814169E-4 0.0 6.495737821628339E-5 6.495737821628339E-5 20 0.0 5.196590257302671E-4 0.0 6.495737821628339E-5 6.495737821628339E-5 21 0.0 5.196590257302671E-4 0.0 6.495737821628339E-5 6.495737821628339E-5 22 0.0 5.196590257302671E-4 0.0 6.495737821628339E-5 6.495737821628339E-5 23 0.0 5.196590257302671E-4 0.0 6.495737821628339E-5 1.2991475643256677E-4 24 0.0 5.196590257302671E-4 0.0 1.2991475643256677E-4 1.9487213464885016E-4 25 0.0 5.196590257302671E-4 0.0 1.2991475643256677E-4 1.9487213464885016E-4 26 0.0 5.196590257302671E-4 0.0 1.9487213464885016E-4 1.9487213464885016E-4 27 0.0 5.196590257302671E-4 0.0 3.247868910814169E-4 1.9487213464885016E-4 28 0.0 5.196590257302671E-4 0.0 7.794885385954006E-4 1.9487213464885016E-4 29 0.0 5.196590257302671E-4 0.0 0.002013678724704785 1.9487213464885016E-4 30 0.0 5.196590257302671E-4 0.0 0.003312826289030453 2.5982951286513355E-4 31 0.0 5.196590257302671E-4 0.0 0.00844445916811684 2.5982951286513355E-4 32 0.0 5.196590257302671E-4 0.0 0.013511134668986945 2.5982951286513355E-4 33 0.0 5.196590257302671E-4 0.0 0.018512852791640767 2.5982951286513355E-4 34 0.0 5.196590257302671E-4 0.0 0.024683803722187685 2.5982951286513355E-4 35 0.0 5.846164039465505E-4 0.0 0.031244498922032307 2.5982951286513355E-4 36 0.0 5.846164039465505E-4 0.0 0.038649640038688614 2.5982951286513355E-4 37 0.0 5.846164039465505E-4 0.0 0.051706073060161575 2.5982951286513355E-4 38 0.0 5.846164039465505E-4 0.0 0.06963430944785579 2.5982951286513355E-4 39 0.0 5.846164039465505E-4 0.0 0.091914690176041 3.247868910814169E-4 40 0.0 5.846164039465505E-4 0.0 0.11588396273784957 3.247868910814169E-4 41 0.0 5.846164039465505E-4 0.0 0.13900878938284644 3.247868910814169E-4 42 0.0 5.846164039465505E-4 0.0 0.16232848816249218 3.247868910814169E-4 43 0.0 5.846164039465505E-4 0.0 0.18870118371830324 3.247868910814169E-4 44 0.0 5.846164039465505E-4 0.0 0.2138396890880049 3.247868910814169E-4 45 0.0 5.846164039465505E-4 0.0 0.23943289610522056 3.247868910814169E-4 46 0.0 5.846164039465505E-4 0.0 0.2721064573480111 3.247868910814169E-4 47 0.0 5.846164039465505E-4 0.0 0.30231163821858287 3.247868910814169E-4 48 0.0 5.846164039465505E-4 0.0 0.3349851994613734 3.897442692977003E-4 49 0.0 5.846164039465505E-4 0.0 0.3683732918645431 3.897442692977003E-4 50 0.0 5.846164039465505E-4 0.0 0.40189129902414533 3.897442692977003E-4 51 0.0 5.846164039465505E-4 0.0 0.43696828326093834 3.897442692977003E-4 52 0.0 5.846164039465505E-4 0.0 0.47379911670957103 3.897442692977003E-4 53 0.0 7.794885385954006E-4 0.0 0.5114743960750153 3.897442692977003E-4 54 0.0 9.094032950279674E-4 0.0 0.551488141056246 3.897442692977003E-4 55 0.0 9.743606732442508E-4 0.0 0.5914369286592602 3.897442692977003E-4 56 0.0 9.743606732442508E-4 0.0 0.6309959719929769 3.897442692977003E-4 57 0.0 9.743606732442508E-4 0.0 0.6739327989939401 3.897442692977003E-4 58 0.0 0.0010393180514605342 0.0 0.719273048988906 3.897442692977003E-4 59 0.0 0.0010393180514605342 0.0 0.7674064662471719 3.897442692977003E-4 60 0.0 0.0010393180514605342 0.0 0.8213860475449034 3.897442692977003E-4 61 0.0 0.0010393180514605342 0.0 0.8780288813495025 3.897442692977003E-4 62 0.0 0.0010393180514605342 0.0 0.9331127380769109 3.897442692977003E-4 63 0.0 0.0010393180514605342 0.0 0.9904701030418891 3.897442692977003E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 1265 0.0 35.995968 1 GGTATCA 745 0.0 28.708193 1 CAACGCA 1685 0.0 25.998365 5 TCAACGC 1720 0.0 25.469326 4 TATCAAC 1770 0.0 24.749855 2 ATCAACG 1775 0.0 24.485807 3 AACGCAG 1775 0.0 23.902811 6 TAGATCG 75 0.0019953805 22.99593 5 AATCGCA 135 2.6650196E-7 22.99593 47 GTACATG 3795 0.0 22.815626 1 TACATGG 3850 0.0 22.040255 2 ACATGGG 3895 0.0 20.722906 3 GCGATAC 185 1.1794327E-8 20.509882 14 TCCGTTA 145 1.26541745E-5 19.034204 65 ACGCAGA 2195 0.0 19.014856 7 GTATATG 400 0.0 18.972874 1 GTATTAT 260 8.913048E-11 18.57484 1 TGCGATA 225 5.5497367E-9 18.396742 13 GTATAGT 245 8.039933E-10 18.304071 1 CCGTTAT 175 3.209676E-6 17.742596 66 >>END_MODULE