FastQCFastQC Report
Mon 6 Feb 2017
SRR3203953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539471
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21800.041.0530131
GGTATCA9600.035.937711
TATCAAC29250.030.5838412
ATCAACG29200.030.0525283
TCAACGC29500.029.5077384
CAACGCA30350.028.669215
AACGCAG31000.028.1781546
ACGCAGA36300.023.8674567
GTACATG39250.023.6717011
CGCAGAG37500.022.831568
TACATGG39900.022.5060582
AGAGTAC38950.021.469411
ACATGGG41100.020.7696383
GAGTACT24400.020.34882712
CAGAGTA41950.020.08110410
ACCGTCG850.004400447520.0652338
AGTACTT24450.020.0277713
GTATAAC1551.1054872E-619.8340361
CGAAATT1056.1116274E-419.67216761
ACGTCGT900.005572926719.26213823
Produced by FastQC (version 0.11.5)