FastQCFastQC Report
Mon 6 Feb 2017
SRR3203952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5994902
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56850.034.6482661
TCAACGC68900.027.2351764
CAACGCA69950.027.171555
ATCAACG70750.026.7180443
AACGCAG74850.024.978026
TATCAAC76800.024.972622
GGTATCA27650.024.5780241
GTACATG132050.022.6493931
TACATGG133750.021.7154482
ACGCAGA84350.021.5923217
ACATGGG138500.019.9993693
CGCAGAG90850.019.439978
AGAGTAC90250.018.00214411
CATGGGG105850.017.7279514
CAGAGTA105300.015.92056310
GCAGAGT112300.015.26606759
ATGGGGG52650.015.0033285
GCGATAC6350.014.66697514
GAGTACT40050.014.55575412
GTATAGA16600.014.5467441
Produced by FastQC (version 0.11.5)