FastQCFastQC Report
Mon 6 Feb 2017
SRR3203952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5994902
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90000.041.8821331
GGTATCA35250.033.8040351
TCAACGC121600.029.963194
ATCAACG123000.029.659543
CAACGCA122900.029.6083565
TATCAAC124550.029.3983142
AACGCAG128700.028.2742616
ACGCAGA150300.024.0191447
CGCAGAG154550.023.182188
GTACATG131650.022.0445041
AGAGTAC156950.021.73066511
GAGTACT110700.020.81655512
TACATGG133400.020.6179582
CAGAGTA170350.020.46812210
GCAGAGT180900.019.5958739
ACATGGG136450.019.2328953
AGTACTT120650.019.10173613
GTACTTT125450.018.23452814
CATGGGG104050.017.4757394
TACTTTT146400.015.718842515
Produced by FastQC (version 0.11.5)