FastQCFastQC Report
Mon 6 Feb 2017
SRR3203951_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203951_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4624970
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47050.039.7378851
TCAACGC56950.030.9512464
ATCAACG56750.030.9387573
CAACGCA57400.030.5283135
AACGCAG60650.028.5511686
GGTATCA20800.028.5252951
TATCAAC63700.028.4296072
ACGCAGA69500.024.4191917
CGCAGAG73200.022.9021478
GTACATG96700.020.7259641
AGAGTAC77400.020.27782811
TACATGG98100.019.9372392
ACATGGG102650.018.448643
CAGAGTA88700.017.88896410
GCAGAGT92900.017.302999
GAGTACT39600.017.16018712
CATGGGG78550.016.7313024
GTATAAG15650.016.0906491
GTATAAT16900.015.3087451
GTATAAA22800.014.8271031
Produced by FastQC (version 0.11.5)