##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3203951_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4624970 Sequences flagged as poor quality 0 Sequence length 75 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.727329690787183 35.0 31.0 35.0 27.0 35.0 2 31.801404117215895 35.0 31.0 35.0 27.0 35.0 3 31.746996845384942 35.0 31.0 35.0 27.0 35.0 4 31.984844009798984 35.0 31.0 35.0 27.0 35.0 5 32.035386175477896 35.0 31.0 35.0 27.0 35.0 6 35.62660579419975 39.0 35.0 40.0 27.0 40.0 7 35.459620927270876 39.0 35.0 40.0 16.0 40.0 8 35.1310616068861 39.0 34.0 40.0 16.0 40.0 9 35.216802054932245 39.0 34.0 40.0 16.0 40.0 10 35.406008038971066 39.0 35.0 40.0 16.0 40.0 11 35.44555489008577 39.0 35.0 40.0 16.0 40.0 12 35.08247664309174 39.0 34.0 40.0 16.0 40.0 13 35.13203739699933 39.0 34.0 40.0 16.0 40.0 14 35.1067388545223 39.0 34.0 40.0 16.0 40.0 15 35.175060162552406 39.0 34.0 40.0 16.0 40.0 16 35.31380765713075 39.0 35.0 40.0 16.0 40.0 17 35.357942429896845 39.0 35.0 40.0 16.0 40.0 18 35.41538799170589 39.0 35.0 40.0 16.0 40.0 19 35.46133920868676 39.0 35.0 40.0 27.0 40.0 20 35.423421124893785 39.0 35.0 40.0 16.0 40.0 21 35.44384201411036 39.0 35.0 40.0 27.0 40.0 22 35.49709814333931 39.0 35.0 40.0 27.0 40.0 23 35.51363360194769 39.0 35.0 40.0 27.0 40.0 24 35.54092372491065 39.0 35.0 40.0 27.0 40.0 25 35.559268492552384 39.0 35.0 40.0 27.0 40.0 26 35.57065516100645 39.0 35.0 40.0 27.0 40.0 27 35.56794638667927 39.0 35.0 40.0 27.0 40.0 28 35.443622120792135 39.0 35.0 40.0 27.0 40.0 29 35.53425168163253 39.0 35.0 40.0 27.0 40.0 30 35.54999664862691 39.0 35.0 40.0 27.0 40.0 31 35.546835763259004 39.0 35.0 40.0 27.0 40.0 32 35.486867158057244 39.0 35.0 40.0 27.0 40.0 33 35.374674646538246 39.0 35.0 40.0 16.0 40.0 34 35.40856308257135 39.0 35.0 40.0 27.0 40.0 35 35.39506526528821 39.0 35.0 40.0 27.0 40.0 36 35.40733929084946 39.0 35.0 40.0 27.0 40.0 37 35.38692013137383 39.0 35.0 40.0 27.0 40.0 38 35.40170076778876 39.0 35.0 40.0 27.0 40.0 39 35.3594362774245 39.0 35.0 40.0 27.0 40.0 40 35.28418800554382 39.0 35.0 40.0 16.0 40.0 41 35.256597340090856 39.0 35.0 40.0 16.0 40.0 42 35.263987442080705 39.0 35.0 40.0 16.0 40.0 43 35.21773935830935 39.0 34.0 40.0 16.0 40.0 44 35.102988127490555 39.0 34.0 40.0 16.0 40.0 45 35.12095494673479 39.0 34.0 40.0 16.0 40.0 46 35.08593763851441 39.0 34.0 40.0 16.0 40.0 47 35.009069464234365 39.0 34.0 40.0 16.0 40.0 48 35.04956680800092 39.0 34.0 40.0 16.0 40.0 49 34.99941859082329 39.0 34.0 40.0 16.0 40.0 50 34.90371267273085 39.0 34.0 40.0 16.0 40.0 51 34.942602438502306 39.0 34.0 40.0 16.0 40.0 52 34.97813283113188 39.0 34.0 40.0 16.0 40.0 53 34.920792351085524 39.0 34.0 40.0 16.0 40.0 54 34.9511845482241 39.0 34.0 40.0 16.0 40.0 55 34.93915441613675 39.0 34.0 40.0 16.0 40.0 56 34.88782889402526 39.0 34.0 40.0 16.0 40.0 57 34.777144284179144 39.0 34.0 40.0 16.0 40.0 58 34.80344413044841 39.0 34.0 40.0 16.0 40.0 59 34.75491992380491 39.0 34.0 40.0 16.0 40.0 60 34.725511949266696 39.0 34.0 40.0 16.0 40.0 61 34.700939033118054 39.0 34.0 40.0 16.0 40.0 62 34.65693290983509 39.0 34.0 40.0 16.0 40.0 63 34.62165354586084 39.0 34.0 40.0 16.0 40.0 64 34.612900408002645 39.0 34.0 40.0 16.0 40.0 65 34.534853415265395 39.0 34.0 40.0 16.0 40.0 66 34.49805274412591 39.0 34.0 40.0 16.0 40.0 67 34.49709835955693 39.0 34.0 40.0 16.0 40.0 68 34.46280083978923 39.0 34.0 40.0 16.0 40.0 69 34.43328605374737 39.0 34.0 40.0 16.0 40.0 70 34.33400735572339 39.0 34.0 40.0 16.0 40.0 71 34.22771520680134 39.0 34.0 40.0 16.0 40.0 72 34.207114208308376 39.0 34.0 40.0 16.0 40.0 73 34.16801125196488 39.0 34.0 40.0 16.0 40.0 74 34.13313880954904 39.0 34.0 40.0 16.0 40.0 75 34.043430551981956 39.0 34.0 40.0 16.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7626.0 3 2160.0 4 2487.0 5 2403.0 6 2312.0 7 2502.0 8 2633.0 9 3120.0 10 3113.0 11 3656.0 12 3945.0 13 4525.0 14 5364.0 15 5997.0 16 6813.0 17 7721.0 18 9869.0 19 19056.0 20 36489.0 21 50144.0 22 59794.0 23 67047.0 24 73928.0 25 80490.0 26 87489.0 27 94552.0 28 101832.0 29 111525.0 30 121859.0 31 134776.0 32 150479.0 33 169237.0 34 191910.0 35 227653.0 36 287152.0 37 402105.0 38 715822.0 39 1365385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.478254288750115 18.418672998940057 13.460009072875778 24.643063639434057 2 14.817371788357548 20.15001178386022 40.65388532249939 24.378731105282846 3 18.038149436644996 25.526414225389573 29.695003427049254 26.74043291091618 4 11.96392625249461 16.875504057323614 39.349530915876215 31.811038774305562 5 13.19989102632017 38.17739358309351 35.489138307924165 13.133577082662157 6 30.89663284302385 38.29962140294964 18.044700830491873 12.75904492353464 7 27.216025185028226 32.435994179421705 22.864364525607733 17.483616109942336 8 25.438521763384408 35.85032983997734 20.836156775070975 17.874991621567276 9 26.143434443899093 16.070547484632332 19.6567977738234 38.12922029764517 10 13.369211043531093 29.492839953556455 35.20550403570185 21.9324449672106 11 34.283833192431516 23.528866133185726 24.60212714893286 17.585173525449896 12 22.818137198727776 25.036011044395963 31.357370966730596 20.788480790145666 13 27.344523315826912 22.5834978389049 26.485447473172798 23.586531372095386 14 21.593869798074365 21.24424590862211 28.084657846429273 29.07722644687425 15 23.06886314938259 29.476191196915874 24.19451369414288 23.260431959558655 16 23.089144362017482 28.567558276053685 25.68129090567074 22.662006456258094 17 21.948238344568004 28.352758332598192 27.24077226047834 22.45823106235546 18 22.09177573043717 28.2664536202397 27.644784722927934 21.996985926395197 19 22.958419189746095 27.70552025202326 27.64549824106967 21.69056231716098 20 23.138096030893173 27.243614553175483 27.590881670583812 22.02740774534754 21 23.2678266021185 27.030683442270977 27.57682752536773 22.124662430242793 22 22.925489246416735 26.97265063340951 27.449648322043167 22.652211798130583 23 22.802980343656284 27.060326877795966 27.29033918057847 22.846353597969284 24 22.488686413101057 27.024434753090294 27.572178846565492 22.91469998724316 25 22.90665669182719 26.996823763181165 27.40311829049702 22.693401254494624 26 22.721725762545486 27.21706302959803 27.359788279707757 22.701422928148723 27 22.843888717116005 27.399485834502713 27.044737587487056 22.711887860894233 28 22.82688557471044 27.2164298762561 27.14538085026402 22.811303698769443 29 22.942202868342928 27.382231668529744 27.096219002501638 22.57934646062569 30 22.988776143412824 27.23230637171701 27.12912732406913 22.649790160801043 31 23.040279180189277 26.960304607381236 27.18681418474066 22.81260202768883 32 22.85597528200183 27.160716718162497 27.232976646335004 22.750331353500673 33 22.867925083430286 27.068471896334866 27.186180751130035 22.877422269104812 34 23.076292718449857 27.092714039009607 27.27120195339941 22.559791289141124 35 23.09313774563148 27.15115588932851 27.158680267340284 22.597026097699725 36 23.065483494260608 27.138096336687372 27.14082068045026 22.65559948860176 37 23.07977715689526 26.89105501066172 27.24372848358839 22.785439348854627 38 22.962875514116334 27.035870411764424 27.182553839780354 22.818700234338884 39 23.0720132567805 27.3518612293225 27.02489581518 22.551229698717005 40 23.192737674771593 26.966106770537724 27.238606129992338 22.60254942469835 41 22.874760348708712 27.023887182323524 27.240646329289252 22.860706139678513 42 22.94280358829758 27.115218999321716 27.071066912897795 22.87091049948291 43 22.817276531928087 26.914310103706935 27.31781664517457 22.95059671919041 44 22.910358030358427 27.113220638611114 27.187491824223564 22.788929506806895 45 23.008103695474542 27.219175567527877 26.94505232190692 22.82766841509066 46 23.092219688144063 27.014116458621444 26.927996720401232 22.965667132833264 47 22.96922159161057 27.133427878233835 27.00707011061494 22.890280419540655 48 22.867482414967842 27.285297416883537 27.02278681137723 22.824433356771394 49 23.072287697806946 26.97608024624624 26.951798976165005 22.99983307978181 50 22.999613401727498 27.121663427716936 27.092171255250864 22.78655191530471 51 23.066007342797963 27.198104191621947 26.76875808118592 22.967130384394178 52 23.12960382758236 27.207761544109825 26.73577867551605 22.926855952791765 53 23.051718635539252 27.17794881153258 26.85489653072856 22.9154360221996 54 22.861478438022043 27.129634794957852 27.11674790166349 22.892138865356614 55 22.86164210111172 27.027177212759447 26.99176026140218 23.119420424726652 56 22.805540047853572 27.329056234651162 27.099215514486453 22.766188203008817 57 23.108522634765443 27.148139987887422 26.811379080780025 22.931958296567114 58 22.910158011006 27.100101601515785 27.09891239458491 22.8908279928933 59 23.122753213124152 27.260278948604284 26.838457077694876 22.77851076057668 60 22.902374795696563 27.37976341343015 27.031017797075414 22.68684399379787 61 23.27144235719492 27.00983144761574 27.017356011099164 22.701370184090173 62 22.97689739148334 27.282708839066196 27.032367695743613 22.70802607370685 63 22.9272407188705 27.052975163689247 27.075051211674488 22.944732905765765 64 22.953959839538047 27.03861296802934 27.061662129989056 22.945765062443552 65 22.985453046842668 27.054288473272635 26.924750145192768 23.035508334691926 66 22.856397196538527 27.2473682412461 26.976116435243146 22.920118126972223 67 22.96946403956864 27.152539257817782 26.87333171166788 23.0046649909457 68 22.93661515628257 27.196578986288788 27.129441354359557 22.737364503069088 69 23.07532135727162 27.24707919435863 26.825053509993975 22.852545938375773 70 22.893980961738386 26.904864045777625 27.129566043186376 23.07158894929761 71 22.90799296756027 26.991668708172995 26.9948904321932 23.105447892073546 72 22.802086603651688 27.238865349804065 26.926947250251466 23.03210079629279 73 22.940725081682125 27.077967048660728 26.98670101953733 22.994606850119816 74 22.996540013846865 27.189367753586367 26.838396029816163 22.975696202750605 75 23.038909797440034 27.1181962342121 26.911597261952796 22.931296706395077 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 272.0 1 342.5 2 479.0 3 616.0 4 687.0 5 612.0 6 540.0 7 596.5 8 650.0 9 849.0 10 1352.0 11 1703.0 12 1750.0 13 1776.0 14 2007.0 15 2471.0 16 2730.0 17 3137.5 18 4070.5 19 5606.5 20 6617.0 21 8204.0 22 12675.0 23 18663.5 24 21768.0 25 26684.0 26 38009.5 27 49866.5 28 55314.0 29 63952.5 30 79975.5 31 94700.0 32 102040.0 33 109437.0 34 125169.0 35 141974.5 36 150445.0 37 156673.0 38 168874.5 39 181848.0 40 188848.0 41 195724.0 42 207094.5 43 216680.5 44 221772.0 45 224534.0 46 229383.0 47 231942.0 48 232414.0 49 232960.0 50 230022.0 51 222097.5 52 217657.0 53 211345.0 54 197807.5 55 181180.0 56 171778.0 57 161872.0 58 139770.0 59 114969.0 60 102364.0 61 91828.5 62 70751.0 63 51478.0 64 42747.0 65 35993.0 66 24231.0 67 16382.5 68 13542.0 69 11374.5 70 7778.5 71 5207.5 72 4065.0 73 3516.0 74 2523.0 75 1591.0 76 1103.0 77 883.5 78 500.5 79 262.5 80 188.0 81 149.0 82 76.0 83 32.0 84 22.0 85 15.5 86 7.0 87 3.5 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004237865326694011 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 4.324352374177562E-5 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0911789698095339 29 0.0 30 0.0 31 0.0 32 0.0 33 0.05509224924702214 34 1.5135233309621467E-4 35 6.486528561266343E-5 36 6.486528561266343E-5 37 2.594611424506537E-4 38 9.513575223190637E-4 39 3.45948189934205E-4 40 1.0810880935443906E-4 41 4.54056999288644E-4 42 0.0010162228079317272 43 3.0270466619242934E-4 44 5.837875705139709E-4 45 9.297357604481759E-4 46 3.0270466619242934E-4 47 1.5135233309621467E-4 48 3.0270466619242934E-4 49 1.2973057122532685E-4 50 3.0270466619242934E-4 51 6.486528561266343E-4 52 3.0270466619242934E-4 53 4.7567876115953183E-4 54 0.002508124377022986 55 0.0014270362834785956 56 6.054093323848587E-4 57 5.837875705139709E-4 58 8.432487129646246E-4 59 6.9189637986841E-4 60 0.0023567720439267715 61 0.0025297461388938737 62 0.0017513627115419126 63 0.0010378445698026148 64 0.0015783886165748103 65 0.001816227997154576 66 0.002508124377022986 67 0.002054067377734342 68 0.003070290185666069 69 0.0028756943288280787 70 0.00384867361301803 71 0.0025297461388938737 72 8.216269510937369E-4 73 6.270310942557466E-4 74 0.0020756891396052296 75 0.0014702798072203712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 4624970.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.02135751376626 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24417641608015 48.52810038871906 2 12.09114810732732 14.99818839032892 3 4.20984551201389 7.833010007350135 4 1.8986578815150594 4.7102935706310225 5 1.041546843805875 3.229907458350953 6 0.6043923478938595 2.2491140332385795 7 0.39129992053251966 1.698826658651901 8 0.27282631734998825 1.3536846854022297 9 0.2016698737312672 1.1257055404598724 >10 0.9804889211060757 10.531469278931304 >50 0.04854209826005729 2.019932497439576 >100 0.01480722633808285 1.4977041680154624 >500 5.985340457484207E-4 0.22406332248098765 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.324352374177562E-5 9 0.0 0.0 0.0 0.0 8.648704748355124E-5 10 0.0 0.0 0.0 0.0 8.648704748355124E-5 11 0.0 0.0 0.0 4.324352374177562E-5 8.648704748355124E-5 12 0.0 0.0 0.0 4.324352374177562E-5 8.648704748355124E-5 13 0.0 0.0 0.0 4.324352374177562E-5 8.648704748355124E-5 14 0.0 0.0 0.0 4.324352374177562E-5 1.0810880935443906E-4 15 0.0 2.162176187088781E-5 0.0 4.324352374177562E-5 1.5135233309621467E-4 16 0.0 4.324352374177562E-5 0.0 4.324352374177562E-5 1.5135233309621467E-4 17 0.0 4.324352374177562E-5 0.0 4.324352374177562E-5 1.5135233309621467E-4 18 0.0 4.324352374177562E-5 0.0 6.486528561266344E-5 1.5135233309621467E-4 19 0.0 4.324352374177562E-5 0.0 6.486528561266344E-5 1.5135233309621467E-4 20 0.0 4.324352374177562E-5 0.0 6.486528561266344E-5 1.5135233309621467E-4 21 0.0 4.324352374177562E-5 0.0 1.0810880935443906E-4 1.5135233309621467E-4 22 0.0 4.324352374177562E-5 0.0 1.0810880935443906E-4 1.5135233309621467E-4 23 0.0 4.324352374177562E-5 0.0 1.2973057122532688E-4 1.729740949671025E-4 24 0.0 4.324352374177562E-5 0.0 1.729740949671025E-4 1.945958568379903E-4 25 0.0 4.324352374177562E-5 0.0 2.1621761870887812E-4 1.945958568379903E-4 26 0.0 4.324352374177562E-5 0.0 2.3783938057976591E-4 1.945958568379903E-4 27 0.0 4.324352374177562E-5 0.0 3.0270466619242934E-4 1.945958568379903E-4 28 0.0 4.324352374177562E-5 0.0 4.54056999288644E-4 1.945958568379903E-4 29 0.0 4.324352374177562E-5 0.0 9.08113998577288E-4 1.945958568379903E-4 30 0.0 4.324352374177562E-5 0.0 0.0017513627115419126 1.945958568379903E-4 31 0.0 4.324352374177562E-5 0.0 0.004043269469856021 1.945958568379903E-4 32 0.0 4.324352374177562E-5 0.0 0.007005450846167651 1.945958568379903E-4 33 0.0 4.324352374177562E-5 0.0 0.010724393887960355 1.945958568379903E-4 34 0.0 4.324352374177562E-5 0.0 0.01353522293117577 1.945958568379903E-4 35 0.0 4.324352374177562E-5 0.0 0.016843352497421606 1.945958568379903E-4 36 0.0 4.324352374177562E-5 0.0 0.021016352538502953 1.945958568379903E-4 37 0.0 4.324352374177562E-5 0.0 0.027524502861640182 1.945958568379903E-4 38 0.0 4.324352374177562E-5 0.0 0.03675699518050928 1.945958568379903E-4 39 0.0 4.324352374177562E-5 0.0 0.04821652897207982 1.945958568379903E-4 40 0.0 6.486528561266344E-5 0.0 0.05915714047874905 2.1621761870887812E-4 41 0.0 6.486528561266344E-5 0.0 0.07087613541277024 2.1621761870887812E-4 42 0.0 6.486528561266344E-5 0.0 0.08222756039498634 2.3783938057976591E-4 43 0.0 6.486528561266344E-5 0.0 0.09398979885274932 2.3783938057976591E-4 44 0.0 6.486528561266344E-5 0.0 0.10800070054508462 2.3783938057976591E-4 45 0.0 8.648704748355124E-5 0.0 0.12218457633238702 2.3783938057976591E-4 46 0.0 8.648704748355124E-5 0.0 0.13688737440459073 2.3783938057976591E-4 47 0.0 8.648704748355124E-5 0.0 0.1522172035710502 2.3783938057976591E-4 48 0.0 8.648704748355124E-5 0.0 0.16944974778214777 2.5946114245065376E-4 49 0.0 8.648704748355124E-5 0.0 0.18795797594362773 2.5946114245065376E-4 50 0.0 8.648704748355124E-5 0.0 0.20746080515116855 2.5946114245065376E-4 51 0.0 8.648704748355124E-5 0.0 0.22663930793064604 2.5946114245065376E-4 52 0.0 8.648704748355124E-5 0.0 0.24741782108856922 2.5946114245065376E-4 53 0.0 8.648704748355124E-5 0.0 0.26921255705442415 4.54056999288644E-4 54 0.0 8.648704748355124E-5 0.0 0.29167756763827657 4.54056999288644E-4 55 0.0 8.648704748355124E-5 0.0 0.3147479875545139 4.54056999288644E-4 56 0.0 8.648704748355124E-5 0.0 0.3396995007535184 4.54056999288644E-4 57 0.0 8.648704748355124E-5 0.0 0.36529966680864956 4.54056999288644E-4 58 0.0 8.648704748355124E-5 0.0 0.3927593043846771 4.54056999288644E-4 59 0.0 8.648704748355124E-5 0.0 0.42002434610386663 4.7567876115953183E-4 60 0.0 8.648704748355124E-5 0.0 0.4518083360540717 4.7567876115953183E-4 61 0.0 8.648704748355124E-5 0.0 0.4840247612416945 4.7567876115953183E-4 62 0.0 8.648704748355124E-5 0.0 0.5184033626164062 4.7567876115953183E-4 63 0.0 8.648704748355124E-5 0.0 0.5538414303227913 4.7567876115953183E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4705 0.0 39.737885 1 TCAACGC 5695 0.0 30.951246 4 ATCAACG 5675 0.0 30.938757 3 CAACGCA 5740 0.0 30.528313 5 AACGCAG 6065 0.0 28.551168 6 GGTATCA 2080 0.0 28.525295 1 TATCAAC 6370 0.0 28.429607 2 ACGCAGA 6950 0.0 24.419191 7 CGCAGAG 7320 0.0 22.902147 8 GTACATG 9670 0.0 20.725964 1 AGAGTAC 7740 0.0 20.277828 11 TACATGG 9810 0.0 19.937239 2 ACATGGG 10265 0.0 18.44864 3 CAGAGTA 8870 0.0 17.888964 10 GCAGAGT 9290 0.0 17.30299 9 GAGTACT 3960 0.0 17.160187 12 CATGGGG 7855 0.0 16.731302 4 GTATAAG 1565 0.0 16.090649 1 GTATAAT 1690 0.0 15.308745 1 GTATAAA 2280 0.0 14.827103 1 >>END_MODULE