FastQCFastQC Report
Mon 6 Feb 2017
SRR3203951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4624970
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80300.040.856211
GGTATCA33050.037.2248881
TCAACGC104350.030.3236664
ATCAACG105100.030.2200813
CAACGCA106300.029.5298355
TATCAAC108500.029.4285622
AACGCAG110500.028.2842276
ACGCAGA125650.024.644417
CGCAGAG130200.023.7834388
GAGTACT99350.022.54350712
AGAGTAC135300.022.01426111
AGTACTT105850.021.38633513
CAGAGTA145850.020.94653110
GCAGAGT147650.020.9230049
GTACTTT109900.020.31819314
GTACATG96200.020.0357881
AATCTCG11300.019.6419736
TACATGG98450.018.8352472
TATGCCG13050.017.95410543
TACTTTT125050.017.88400315
Produced by FastQC (version 0.11.5)