FastQCFastQC Report
Mon 6 Feb 2017
SRR3203950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3959034
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39850.036.097581
TCAACGC48000.028.960914
ATCAACG48750.028.4446013
CAACGCA49000.028.3698735
GGTATCA19150.027.921151
AACGCAG51300.026.6944856
TATCAAC53800.025.7746162
ACGCAGA60150.022.308097
CGCAGAG62050.021.2914588
GTACATG83200.021.2283881
TACATGG83050.019.936552
AGAGTAC61750.019.71905911
ACATGGG82750.019.0500723
TAGTCCG1351.580136E-417.8859795
CAGAGTA71550.017.69313610
GCAGAGT76200.017.1566569
CGGTTCG2057.778981E-716.8265342
GTATAGC11700.015.6264521
TAGCCCT24250.015.5046924
CATGGGG58900.015.4609824
Produced by FastQC (version 0.11.5)