FastQCFastQC Report
Mon 6 Feb 2017
SRR3203950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3959034
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63600.041.3005181
GGTATCA25000.034.2941441
ATCAACG82200.030.5237583
TCAACGC81500.030.480394
TATCAAC85700.029.9126012
CAACGCA83200.029.7661615
AACGCAG85850.028.8079746
ACGCAGA100350.024.3623527
CGCAGAG100750.024.1979438
AGAGTAC104950.022.1333311
GAGTACT74400.021.90391512
CAGAGTA114950.020.81811310
GCAGAGT117500.020.3380749
GTACATG86000.020.1767651
TACATGG85450.019.9734462
GTACTTT82200.019.57603814
AGTACTT83400.019.54043213
TCGTATG20850.019.11910640
ACATGGG87200.018.947483
CCGTCTT20400.018.85753847
Produced by FastQC (version 0.11.5)