FastQCFastQC Report
Mon 6 Feb 2017
SRR3203949_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203949_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096601
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38800.035.6509551
GGTATCA18100.029.5400771
TCAACGC47800.028.6483824
CAACGCA48350.028.2511525
ATCAACG48900.028.0744783
AACGCAG49950.027.4843246
TATCAAC51900.026.252292
ACGCAGA57650.023.09547
CGCAGAG61350.021.42148
AGAGTAC59000.020.93030211
GTACATG83800.020.4995271
TACATGG83050.020.0606482
GTATAGC10350.019.6639231
CAGAGTA65300.019.17480510
ACATGGG87350.018.599253
GCAGAGT70500.018.0540549
GAGTACT25250.017.89593512
GCGATAC2351.823737E-716.14616214
CATGGGG57050.016.1433284
TTCGTCG1300.002525083315.92111339
Produced by FastQC (version 0.11.5)