FastQCFastQC Report
Mon 6 Feb 2017
SRR3203949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096601
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51400.042.81311
GGTATCA20450.035.089721
ATCAACG70850.030.1254883
TCAACGC70950.030.1145954
CAACGCA71750.029.5261765
TATCAAC74000.029.257042
AACGCAG74250.028.532036
ACGCAGA85050.024.5745267
CGCAGAG86550.023.9126458
GTACATG82800.023.7296561
TACATGG83350.023.0252082
AGAGTAC89400.022.47762311
TCGTATG14700.022.39778540
CTCGTAT13700.022.26292839
GAGTACT57750.022.118412
ACATGGG86100.021.338953
TATGCCG15550.021.26924943
CAGAGTA96100.021.17945910
AGTACTT62450.020.45476213
GCAGAGT99800.020.428419
Produced by FastQC (version 0.11.5)