FastQCFastQC Report
Mon 6 Feb 2017
SRR3203948_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203948_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4373133
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38650.035.5209471
ATCAACG45200.028.3877263
TCAACGC45350.028.293834
CAACGCA46250.027.6686695
AACGCAG48500.026.1005956
TATCAAC51550.025.827642
ACGCAGA55800.021.882417
GTACATG91400.021.7383821
TACATGG92450.020.6321432
CGCAGAG59300.020.241878
ACATGGG94200.019.7728373
GGTATCA18800.019.6325551
AGAGTAC62000.018.8596711
GCGATAC5350.018.05220814
GCGAACG1200.001479455617.24689935
CAGAGTA71550.016.6316610
TGCGATA6050.016.5336513
CATGGGG70500.016.2922574
CGGTATG5150.016.07551459
GTGCGAT6450.015.50830712
Produced by FastQC (version 0.11.5)