FastQCFastQC Report
Mon 6 Feb 2017
SRR3203948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4373133
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59200.039.022341
GGTATCA25400.036.998771
TCAACGC78150.028.3955254
ATCAACG79200.028.070533
CAACGCA78650.028.0288645
TATCAAC80850.027.7920652
AACGCAG81900.026.9585846
TATCTCG11650.023.8085336
ACGCAGA94250.023.5220497
GTACATG91250.022.5076371
CGCAGAG98350.022.5070468
ACGGACT15150.022.18941927
TACATGG92650.021.2669542
CTCGTAT15200.020.75467339
AGAGTAC100650.020.71354311
TATGCCG15950.020.53192143
TCGTATG16150.020.17754640
ACATGGG94850.020.0185343
GACGGAC17000.019.77514826
CGTGCCG6300.019.48791710
Produced by FastQC (version 0.11.5)