FastQCFastQC Report
Mon 6 Feb 2017
SRR3203947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3261754
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33700.037.9772531
GGTATCA15500.029.8230881
ATCAACG43750.027.3588393
TCAACGC43950.027.077374
CAACGCA45750.025.7104425
TATCAAC49300.024.90862
AACGCAG47250.024.8942386
GTACATG71200.023.0625061
ACGCAGA52550.021.9240117
CGCAGAG53350.021.271978
TACATGG73100.021.187282
TAGCGCG850.004125785520.2907124
ACATGGG74350.019.6248173
CGTGCCG2901.8189894E-1219.03128810
AGAGTAC56300.018.99326311
TTTCACG8050.018.01520724
CATGGGG47800.017.8244154
GCCGCTC8450.017.55353744
TTAGTCG3451.8189894E-1216.99766739
CAGAGTA64400.016.92573210
Produced by FastQC (version 0.11.5)