FastQCFastQC Report
Mon 6 Feb 2017
SRR3203947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3261754
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.044.5194321
GTATCAA69250.041.986041
TCAACGC92750.030.3269164
ATCAACG93850.030.2740613
TATCAAC97750.029.4500332
CAACGCA95850.029.1883855
AACGCAG98200.028.455586
ACGCAGA110650.025.1158647
GGCGAAT11900.024.19476332
CGCAGAG114550.024.142068
TATGCCG13450.022.7992843
AGAGTAC116450.022.59140411
GAGTACT90400.022.50168812
CAGAGTA123350.021.69664210
AGTACTT96350.021.64591813
GTACATG76350.021.2559071
GCAGAGT127650.021.0191889
CTCGTAT13150.020.82216839
GCCGTCT14700.020.74477646
CGTCCGA2151.6189006E-1020.63161711
Produced by FastQC (version 0.11.5)