FastQCFastQC Report
Mon 6 Feb 2017
SRR3203946_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203946_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819684
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43050.038.5424841
GGTATCA18850.029.8293671
TCAACGC53400.029.585234
ATCAACG54100.029.0749033
CAACGCA54200.028.7666875
TATCAAC56800.028.4823112
AACGCAG57100.027.2452726
CGAATCG2950.023.38645437
ACGCAGA67250.022.8766947
GTACATG97950.021.694221
CGCAGAG70500.021.3328118
TACATGG100400.020.9578532
AGAGTAC68300.020.75734511
ACATGGG100800.019.8822863
CAGAGTA79000.018.42621210
GCAGAGT82100.017.6044149
CATGGGG71350.016.824264
CGACACG4200.016.45074827
GTATATA17150.015.8902491
GTATAAA18150.015.7749951
Produced by FastQC (version 0.11.5)