FastQCFastQC Report
Mon 6 Feb 2017
SRR3203946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819684
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70650.044.101311
GGTATCA26500.037.387031
TATCAAC99150.030.989662
TCAACGC98500.030.8692724
ATCAACG99950.030.4687613
CAACGCA100000.030.2253065
AACGCAG102900.029.373866
ACGCAGA119600.024.8353567
CGCAGAG122700.024.0411348
AGAGTAC123250.023.02981411
CGTACTA13350.022.58803229
GAGTACT89950.022.2697812
CAGAGTA132350.022.12928210
GACCGTA14100.021.87789726
GTACATG97750.021.4595411
ACCGTAC14250.021.4043727
AGTACTT95000.021.23042113
GCAGAGT138100.021.2078979
TACATGG96700.020.6536312
GTACTTT97300.020.55314814
Produced by FastQC (version 0.11.5)