FastQCFastQC Report
Mon 6 Feb 2017
SRR3203945_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203945_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305240
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37750.033.3540041
ATCAACG46750.026.19323
GGTATCA20200.026.1283571
TCAACGC47400.025.6884674
CAACGCA48350.025.2550745
TATCAAC50000.024.6286162
AACGCAG51750.023.7291136
GTACATG91550.021.213981
TACATGG91850.020.2794632
ACGCAGA60200.020.2264697
CGCAGAG61700.019.34348
ACATGGG95500.018.9625953
AGAGTAC63100.017.5475911
CATGGGG79200.016.3758754
TAGGCCG4100.016.00038323
CAGAGTA71650.015.88691510
GTATTAC7950.015.18703651
GAGTACA63850.014.9654891
GTATAGG10150.014.6141821
CGCACGA4750.014.52373135
Produced by FastQC (version 0.11.5)