FastQCFastQC Report
Mon 6 Feb 2017
SRR3203945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305240
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58150.043.1352961
GGTATCA22050.032.3469161
TCAACGC81000.029.753954
ATCAACG82000.029.5302313
TATCAAC85000.029.2095382
CAACGCA82650.029.1899975
AACGCAG87050.027.7537126
ACGCAGA99100.024.3685427
CGCAGAG103550.023.2886948
AGAGTAC104650.022.1133711
GTACATG91400.021.9919781
GAGTACT74650.020.94130712
TACATGG90800.020.9209582
CAGAGTA113750.020.83791410
CGTACTA14300.020.61676229
CGAATCG3700.019.84197837
GACCGTA15250.019.78732126
ACCGTAC15450.019.7556727
GCAGAGT121300.019.6529889
ACATGGG93200.019.5044483
Produced by FastQC (version 0.11.5)