FastQCFastQC Report
Mon 6 Feb 2017
SRR3203944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3601041
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37350.039.52781
TCAACGC45550.030.5182884
ATCAACG46900.029.4191843
CAACGCA49000.028.5103445
TATCAAC49600.028.3045482
GGTATCA17500.026.6100251
AACGCAG52350.026.5541156
CGAATCG2500.026.21652837
ACGCAGA58550.023.3887567
GTACATG84800.022.9014021
CGCAGAG62300.021.5933518
TACATGG88450.021.098082
ACATGGG89050.020.3748913
AGAGTAC63400.019.69530911
CAGAGTA71250.018.15473410
GCAGAGT75400.017.3384939
GCGATAC4100.016.8263414
GAGTACT31850.016.67841512
CATGGGG57300.016.6751084
GTATAAT16300.016.5065571
Produced by FastQC (version 0.11.5)