FastQCFastQC Report
Mon 6 Feb 2017
SRR3203944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3601041
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60400.041.5795021
GGTATCA23250.037.0344961
TATCAAC81150.030.7969062
ATCAACG81550.030.4796583
TCAACGC81500.030.4048444
CAACGCA83100.029.763885
AACGCAG85500.028.8086996
ACGCAGA95150.025.8030597
CGCAGAG99550.024.3885618
GTACATG80650.022.2848781
AGAGTAC103250.022.20495411
CAGAGTA109150.021.39824310
GAGTACT75200.020.94130112
TACATGG84350.020.8452322
GCAGAGT113250.020.7743859
ACATGGG82350.020.3573173
AGTACTT80400.019.71600213
GTACTTT79900.019.45400614
CGCACGA4000.019.0768435
TATGCCG15850.018.9119243
Produced by FastQC (version 0.11.5)