FastQCFastQC Report
Mon 6 Feb 2017
SRR3203943_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203943_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578583
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17300.041.275231
TATCAAC23150.030.6950072
CAACGCA23200.029.8854335
TCAACGC24050.029.2594784
ATCAACG24100.029.0556433
AACGCAG25450.027.2433036
GGTATCA7850.026.8055921
CGAATCG1151.7006187E-623.99626537
GTACATG44350.023.1008551
GTATACG750.001994787522.9971491
ACGCAGA29950.022.5741147
TATACTA1554.0956365E-822.25465
TACATGG44050.022.2394452
GCTATAC2900.021.410463
TCGCAAT1808.602001E-921.08005149
GATCGCG1004.3403474E-420.6967855
CGCAGAG32500.020.5906418
ACATGGG46250.020.510323
AGCGTAC3050.020.37297423
GTATAGA3050.020.3581311
Produced by FastQC (version 0.11.5)