FastQCFastQC Report
Mon 6 Feb 2017
SRR3203943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578583
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27900.040.0244521
GGTATCA13050.038.2053531
TATCTCG9050.030.26522636
TATCAAC37550.029.4442182
TCAACGC36950.029.2684424
ATCAACG37500.029.0294043
CAACGCA37850.028.7427355
AACGCAG38850.028.0915726
ATCTCGT11050.026.2317637
CCGTCTT12300.025.93857247
TCTCGTA11300.025.78221138
ACGGACT12650.025.75341227
CTCGTAT12000.025.72338539
TATGCCG12600.025.44530543
TCGTATG13100.024.88719440
CCTAATA1401.5601472E-824.3745652
ACGCAGA44900.024.143017
CCTATCT11350.024.13140534
ACTCCTA13400.023.8046831
CGGACTC13900.023.68755728
Produced by FastQC (version 0.11.5)