##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203943_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1578583
Sequences flagged as poor quality	0
Sequence length	75
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55225287488843	35.0	32.0	35.0	27.0	35.0
2	32.69093991256716	35.0	32.0	35.0	27.0	35.0
3	32.64019060131776	35.0	32.0	35.0	27.0	35.0
4	32.792989028768204	35.0	33.0	35.0	30.0	35.0
5	32.831877069498404	35.0	32.0	35.0	30.0	35.0
6	36.31938263619968	39.0	36.0	40.0	27.0	40.0
7	36.12729454200381	39.0	36.0	40.0	27.0	40.0
8	35.98892171016665	39.0	36.0	40.0	27.0	40.0
9	35.98279723017415	39.0	36.0	40.0	27.0	40.0
10	35.931878779893104	39.0	36.0	40.0	27.0	40.0
11	36.015093283026616	39.0	36.0	40.0	27.0	40.0
12	36.00905242233066	39.0	36.0	40.0	27.0	40.0
13	35.99194720835078	39.0	36.0	40.0	27.0	40.0
14	35.92154039413829	39.0	36.0	40.0	27.0	40.0
15	35.87366517946791	39.0	36.0	40.0	27.0	40.0
16	35.87044837046896	39.0	36.0	40.0	27.0	40.0
17	35.56369351500681	39.0	35.0	40.0	27.0	40.0
18	35.52547886300562	39.0	35.0	40.0	27.0	40.0
19	35.74571498616164	39.0	35.0	40.0	27.0	40.0
20	35.726085989776905	39.0	35.0	40.0	27.0	40.0
21	35.49348941424049	39.0	35.0	40.0	27.0	40.0
22	35.486075169946716	39.0	35.0	40.0	27.0	40.0
23	35.284622981496696	39.0	35.0	40.0	16.0	40.0
24	35.321396467591505	39.0	35.0	40.0	27.0	40.0
25	35.075276371277276	39.0	35.0	40.0	16.0	40.0
26	35.11832510549018	39.0	35.0	40.0	16.0	40.0
27	35.21875948239656	39.0	35.0	40.0	16.0	40.0
28	35.191345022719744	39.0	34.0	40.0	16.0	40.0
29	35.17601925270955	39.0	35.0	40.0	16.0	40.0
30	35.10165635889909	39.0	34.0	40.0	16.0	40.0
31	35.12946294239834	39.0	34.0	40.0	16.0	40.0
32	34.919256700471244	39.0	34.0	40.0	16.0	40.0
33	35.13376046745721	39.0	34.0	40.0	16.0	40.0
34	35.15604247606873	39.0	34.0	40.0	16.0	40.0
35	35.202588650707625	39.0	35.0	40.0	16.0	40.0
36	35.19146031599225	39.0	35.0	40.0	16.0	40.0
37	34.99591532405962	39.0	34.0	40.0	16.0	40.0
38	35.03887663809885	39.0	34.0	40.0	16.0	40.0
39	35.075921253427914	39.0	34.0	40.0	16.0	40.0
40	35.150483059807435	39.0	35.0	40.0	16.0	40.0
41	35.278488999311406	39.0	35.0	40.0	27.0	40.0
42	34.99079554258471	39.0	34.0	40.0	16.0	40.0
43	34.9421145419658	39.0	34.0	40.0	16.0	40.0
44	34.99517985433772	39.0	35.0	40.0	16.0	40.0
45	34.827214026756906	39.0	34.0	40.0	16.0	40.0
46	34.75076191749183	39.0	34.0	40.0	16.0	40.0
47	34.84915775730513	39.0	34.0	40.0	16.0	40.0
48	34.7389848997487	39.0	34.0	40.0	16.0	40.0
49	34.768748301483036	39.0	34.0	40.0	16.0	40.0
50	34.688599839222896	39.0	34.0	40.0	16.0	40.0
51	34.34683320420909	39.0	34.0	40.0	16.0	40.0
52	34.6624536055437	39.0	34.0	40.0	16.0	40.0
53	34.67788199923602	39.0	34.0	40.0	16.0	40.0
54	34.621139971734145	39.0	34.0	40.0	16.0	40.0
55	34.53441599206377	39.0	34.0	40.0	16.0	40.0
56	34.563929802867506	39.0	34.0	40.0	16.0	40.0
57	34.5124082800841	39.0	34.0	40.0	16.0	40.0
58	34.55102139070293	39.0	34.0	40.0	16.0	40.0
59	34.51223280625726	39.0	34.0	40.0	16.0	40.0
60	34.18678016930374	39.0	34.0	40.0	16.0	40.0
61	34.44492560733265	39.0	34.0	40.0	16.0	40.0
62	34.16570747309454	39.0	34.0	40.0	16.0	40.0
63	34.33415981294617	39.0	34.0	40.0	16.0	40.0
64	34.33050273568131	39.0	34.0	40.0	16.0	40.0
65	34.267048359193026	39.0	34.0	40.0	16.0	40.0
66	34.27141556699901	39.0	34.0	40.0	16.0	40.0
67	34.24768035637024	39.0	34.0	40.0	16.0	40.0
68	34.14089217988538	39.0	34.0	40.0	16.0	40.0
69	34.026412928556816	39.0	34.0	40.0	16.0	40.0
70	33.92709981039958	39.0	34.0	40.0	16.0	40.0
71	33.874007258408334	39.0	34.0	40.0	16.0	40.0
72	33.892658795894796	39.0	34.0	40.0	16.0	40.0
73	33.71013180808358	39.0	34.0	40.0	16.0	40.0
74	33.62961909509985	39.0	34.0	40.0	16.0	40.0
75	33.553880917253004	39.0	34.0	40.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	529.0
3	1098.0
4	943.0
5	870.0
6	1072.0
7	1441.0
8	2190.0
9	3691.0
10	3332.0
11	2530.0
12	2529.0
13	2811.0
14	1759.0
15	1967.0
16	2387.0
17	2797.0
18	3262.0
19	4608.0
20	8337.0
21	13266.0
22	17515.0
23	21891.0
24	26270.0
25	29755.0
26	31476.0
27	34437.0
28	37718.0
29	40954.0
30	44552.0
31	48179.0
32	52127.0
33	57868.0
34	64933.0
35	73641.0
36	89882.0
37	121790.0
38	207995.0
39	516181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.18890473926137	15.449548264384214	11.816452686638137	27.545094309716276
2	16.412694169391155	18.165848739027343	36.64362279335328	28.77783429822822
3	19.574904160775407	22.25307892817239	27.132149441378843	31.03986746967336
4	13.39669373299904	15.215115232607367	33.52348635993743	37.864704674456156
5	15.673501605264558	35.844024538882366	32.033009915391034	16.449463940462042
6	35.590042743493406	33.84747215081405	15.711149308143574	14.851335797548968
7	31.25746318058664	29.763465082292157	18.987218283739278	19.991853453381925
8	28.496591889723966	32.85040683168028	18.202882352717552	20.450118925878193
9	27.76151400293065	14.09016586093934	16.39736938633163	41.750950749798385
10	15.321978839236733	27.656973156921843	31.67279241749874	25.348255586342678
11	37.60052604539169	20.750666267149214	21.516802221756258	20.13200546570284
12	25.61774733649318	22.378841955759622	28.05262777801006	23.95078292973714
13	30.37209205092486	19.325955728033318	23.911904576383176	26.390047644658647
14	24.099296566962973	18.27367498150194	24.72132547461458	32.9057029769205
15	25.591329594385627	25.93006482658745	21.95830395452953	26.520301624497396
16	26.106234775750163	25.166472915485414	23.00902447600072	25.71826783276369
17	24.686620829904875	25.212205273974604	24.19216193909027	25.909011957030252
18	24.93605674459019	24.49740696661847	25.018748843105737	25.547787445685604
19	25.82689728104908	24.2665308173327	25.111724038298867	24.79484786331935
20	25.827444063019943	23.78701306600671	25.09538780480696	25.29015506616638
21	25.968374367690917	23.608601948071776	25.205786709550033	25.217236974687278
22	25.63383238389679	23.767275133032438	25.123140487981754	25.475751995089013
23	25.607148004035164	23.35226017197483	25.04645241869626	25.994139405293748
24	25.325881692441175	23.64543459398765	25.27420507324044	25.75447864033073
25	25.50940002100419	23.58783844295783	25.1600603322915	25.742701203746478
26	25.536999413297394	23.999210897808705	25.140462752070754	25.323326936823147
27	25.417315639465908	24.136412909236807	25.10088140851307	25.34539004278421
28	25.379352727420784	23.98873202161986	24.940008448439073	25.691906802520283
29	25.693376566780508	23.778651917636314	24.88079765582055	25.647173859762624
30	25.53878820729451	23.72351175296472	25.239420539898518	25.49827949984225
31	25.18494956788376	23.771259896368445	24.97724113581205	26.066549399935745
32	25.025044131474644	23.924575530794673	25.105300644870372	25.945079692860308
33	25.300786311537944	23.638550361988845	25.48179666800725	25.578866658465966
34	25.491479620366462	23.920742370718422	24.9163293439877	25.67144866492741
35	25.38356578298691	23.641735977481275	25.301124345986864	25.673573893544948
36	25.623141268403245	23.666339071058367	24.947547354638786	25.762972305899602
37	25.444188688767543	23.70541301126051	25.20382531929965	25.6465729806723
38	25.579143846770595	23.708824205054963	25.10249356755681	25.60953838061763
39	25.721678557885646	23.96798133944305	24.67941507311586	25.630925029555442
40	25.59186240778694	23.486119011710237	25.53133084339153	25.39068773711129
41	25.35093996380718	23.706361094944135	24.95186482076921	25.990834120479477
42	25.725211012649563	23.611277299191656	25.212100426088462	25.451411262070323
43	25.533161666234733	23.426494863391884	25.247127852837803	25.793215617535576
44	25.519827692828244	24.11665979907323	24.59773311741534	25.765779390683186
45	25.384742098611767	23.83769629844933	24.91478972986484	25.862771873074063
46	25.875454828190858	23.648203869879545	24.418605390711807	26.057735911217794
47	25.353597928085957	23.783380165951563	24.8583891020985	26.004632803863974
48	25.23387786643092	24.014641018383955	24.690659195634407	26.06082191955072
49	25.779438101386027	23.57246343300554	24.740313998812827	25.907784466795604
50	25.731018973108235	23.776567102720854	24.983940958474548	25.508472965696367
51	25.23553802316279	24.04709347941378	24.386933402161674	26.330435095261763
52	25.528951370245924	23.87348797214797	24.706148727762802	25.891411929843304
53	25.400824130919663	24.020954981896214	24.814875823860618	25.763345063323502
54	25.22802538696422	23.959249584844653	24.644677266404756	26.168047761786372
55	25.528279750726007	23.701103175700695	24.77662141847072	25.993995655102577
56	25.309095977016955	24.21651447051259	24.723687169594978	25.750702382875478
57	25.59072102839528	23.890159052337705	24.351472280979163	26.167647638287857
58	25.310632484372974	24.03795516817658	24.756722102226327	25.894690245224123
59	25.446001240396903	24.119828168919504	24.65950851648404	25.774662074199554
60	25.542306126961645	24.328514614785593	24.595589766926135	25.533589491326623
61	25.445378215142288	24.308255825297458	24.652053218594833	25.59431274096542
62	25.237826695086714	24.67968508553095	24.33768873680555	25.74479948257679
63	25.022013873491495	24.41804187387159	24.63849735516772	25.921446897469195
64	25.411319204309052	24.33889051776389	24.4278037726441	25.82198650528296
65	25.46789701709479	24.370025278641748	24.404817661786176	25.75726004247729
66	25.25902517584062	24.48806336471074	24.489773938209016	25.76313752123962
67	25.470570234377526	24.31575739490139	24.349463459015137	25.864208911705948
68	25.376119552388115	24.49644296420283	24.479590679546202	25.64784680386285
69	25.398593267580654	24.349395797103845	24.371340212218396	25.880670723097097
70	25.33550951943336	24.032984387288046	24.542956307604456	26.088549785674136
71	25.432456887374922	23.99657329399718	24.463816823301602	26.1071529953263
72	25.15989718141184	24.322727484214237	24.518396381846262	25.99897895252767
73	25.3969279779815	23.948542395690577	24.464601038199742	26.18992858812818
74	25.411196262579395	24.212853783018414	24.41073228485033	25.96521766955186
75	25.311967139334545	24.27835214919148	24.43080180277262	25.978878908701358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	85.0
1	74.0
2	123.0
3	206.5
4	230.0
5	184.5
6	175.5
7	305.0
8	398.0
9	701.5
10	1325.5
11	1552.0
12	1458.0
13	1395.0
14	1389.0
15	1461.0
16	1476.0
17	1601.0
18	1810.5
19	2041.5
20	2188.0
21	2421.0
22	2887.0
23	3326.5
24	3533.0
25	3813.0
26	4514.5
27	5218.0
28	5500.0
29	5896.5
30	6727.0
31	7656.5
32	8152.0
33	8837.5
34	10357.0
35	12173.0
36	13155.0
37	14544.5
38	17801.5
39	22724.0
40	25779.0
41	29645.0
42	38870.0
43	50063.5
44	55898.0
45	61843.5
46	72802.0
47	83074.0
48	88333.0
49	93286.0
50	101201.0
51	105887.0
52	107611.0
53	107023.0
54	106476.0
55	103485.5
56	100454.0
57	97746.0
58	88591.5
59	74388.5
60	66632.0
61	61155.5
62	48227.0
63	35360.0
64	29945.0
65	25097.5
66	16774.5
67	11400.0
68	9501.0
69	7921.5
70	5252.0
71	3413.5
72	2665.0
73	2285.0
74	1632.5
75	1056.5
76	753.0
77	617.0
78	363.0
79	185.5
80	126.0
81	95.0
82	46.5
83	21.0
84	13.0
85	8.0
86	2.5
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08444281992141053
2	0.0
3	0.04307660731174731
4	0.046307352860128354
5	0.001710394702084084
6	0.05061501359130308
7	0.0
8	0.018307558107492607
9	0.005828011577471695
10	6.334795192904017E-5
11	7.601754231484819E-4
12	0.0015836987982260039
13	0.0013936549424388835
14	0.002470570125232566
15	0.004054268923458571
16	0.006208099289045936
17	0.06974609507387321
18	0.4108748162117545
19	0.023058654502170615
20	0.018307558107492607
21	0.4160060003180067
22	0.41796978682780694
23	1.096426351987827
24	0.7058862283452945
25	1.6794809015427126
26	1.0968697876513305
27	1.00450847373879
28	0.42411453816492384
29	1.0074858274794547
30	1.0107799209797648
31	0.4224041434628398
32	1.0983901384976273
33	0.608900513941934
34	0.4210104885204009
35	0.4154358687506453
36	1.0577841013111127
37	1.0893947293237036
38	1.0010243363826925
39	0.6016788474220235
40	0.998490418305531
41	0.4084675940384509
42	0.998490418305531
43	0.7592252038695463
44	1.1474848012426333
45	0.39883870534523685
46	0.3982052258259464
47	0.4001690123357467
48	0.3983319217298045
49	0.0026606139810196865
50	0.0020904824136583253
51	1.3356915664238118
52	0.39744505040279793
53	0.39655817907579144
54	0.3978251381143722
55	0.5945838768059709
56	0.0017737426540131245
57	0.0029140057887358474
58	0.3995355328164563
59	0.003547485308026249
60	1.1623082219940288
61	0.0023438742213744857
62	0.5871088184783442
63	0.0020904824136583253
64	0.3968115708835075
65	0.5874889061899184
66	0.010452412068291625
67	0.014696724847537316
68	0.010262368212504506
69	0.4072639829517992
70	0.6003485404315135
71	0.0247690492042547
72	0.6085204262303597
73	0.017357338828557004
74	1.0140740144800748
75	0.06417147530411768
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	1578583.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.36835339861241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34780602466908	52.52203779023728
2	11.075097707434306	14.479218017274556
3	3.8368534668522534	7.524263800796679
4	1.7035059624441307	4.454215190787652
5	0.8941701014112177	2.922521359376079
6	0.5418093013825782	2.1250309132459004
7	0.3536500881571988	1.6182266759477142
8	0.2395975576715067	1.2529678258652377
9	0.17257640511475184	1.015293189402288
>10	0.7792281033318001	8.721134252561793
>50	0.042570951562882535	1.8756408241154783
>100	0.012936847177652479	1.4061863051096009
>500	1.9748279065335143E-4	0.08326385527974242
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.334795192904015E-5	0.0
6	0.0	0.0	0.0	6.334795192904015E-5	6.334795192904015E-5
7	0.0	0.0	0.0	6.334795192904015E-5	6.334795192904015E-5
8	0.0	0.0	0.0	6.334795192904015E-5	6.334795192904015E-5
9	0.0	0.0	0.0	6.334795192904015E-5	6.334795192904015E-5
10	0.0	0.0	0.0	6.334795192904015E-5	6.334795192904015E-5
11	0.0	0.0	0.0	6.334795192904015E-5	6.334795192904015E-5
12	0.0	0.0	0.0	6.334795192904015E-5	6.334795192904015E-5
13	0.0	0.0	0.0	6.334795192904015E-5	6.334795192904015E-5
14	0.0	0.0	0.0	6.334795192904015E-5	1.266959038580803E-4
15	0.0	0.0	0.0	6.334795192904015E-5	3.8008771157424097E-4
16	0.0	0.0	0.0	6.334795192904015E-5	3.8008771157424097E-4
17	0.0	0.0	0.0	6.334795192904015E-5	3.8008771157424097E-4
18	0.0	0.0	0.0	6.334795192904015E-5	3.8008771157424097E-4
19	0.0	0.0	0.0	6.334795192904015E-5	3.8008771157424097E-4
20	0.0	0.0	0.0	6.334795192904015E-5	3.8008771157424097E-4
21	0.0	0.0	0.0	6.334795192904015E-5	3.8008771157424097E-4
22	0.0	0.0	0.0	1.266959038580803E-4	3.8008771157424097E-4
23	0.0	0.0	0.0	3.1673975964520076E-4	6.968274712194417E-4
24	0.0	0.0	0.0	6.968274712194417E-4	7.601754231484819E-4
25	0.0	0.0	0.0	7.601754231484819E-4	7.601754231484819E-4
26	0.0	0.0	0.0	0.0010135672308646424	7.601754231484819E-4
27	0.0	0.0	0.0	0.0010769151827936827	7.601754231484819E-4
28	0.0	0.0	0.0	0.0022171783175164057	7.601754231484819E-4
29	0.0	0.0	0.0	0.0031673975964520078	7.601754231484819E-4
30	0.0	0.0	0.0	0.005891359529400735	7.601754231484819E-4
31	0.0	0.0	0.0	0.012542894481949951	7.601754231484819E-4
32	0.0	6.334795192904015E-5	0.0	0.02071478028079613	8.868713270065622E-4
33	0.0	6.334795192904015E-5	0.0	0.02818983860842287	8.868713270065622E-4
34	0.0	6.334795192904015E-5	0.0	0.03604498464762385	8.868713270065622E-4
35	0.0	6.334795192904015E-5	0.0	0.04504039382154755	8.868713270065622E-4
36	0.0	6.334795192904015E-5	0.0	0.05751994035156846	8.868713270065622E-4
37	0.0	6.334795192904015E-5	0.0	0.07462388737240931	8.868713270065622E-4
38	0.0	6.334795192904015E-5	0.0	0.09717575825914761	8.868713270065622E-4
39	0.0	6.334795192904015E-5	0.0	0.11979097709781494	9.502192789356023E-4
40	0.0	6.334795192904015E-5	0.0	0.1446867222059277	9.502192789356023E-4
41	0.0	6.334795192904015E-5	0.0	0.16844220417931777	9.502192789356023E-4
42	0.0	6.334795192904015E-5	0.0	0.19327460133550153	9.502192789356023E-4
43	0.0	6.334795192904015E-5	0.0	0.21791695463589814	9.502192789356023E-4
44	0.0	6.334795192904015E-5	0.0	0.24585340143660486	9.502192789356023E-4
45	0.0	6.334795192904015E-5	0.0	0.2773373335453378	9.502192789356023E-4
46	0.0	6.334795192904015E-5	0.0	0.3078710463751352	9.502192789356023E-4
47	0.0	6.334795192904015E-5	0.0	0.3387214989645777	9.502192789356023E-4
48	0.0	6.334795192904015E-5	0.0	0.37330948071783365	9.502192789356023E-4
49	0.0	6.334795192904015E-5	0.0	0.40606037186514743	9.502192789356023E-4
50	0.0	6.334795192904015E-5	0.0	0.44128183313769376	9.502192789356023E-4
51	0.0	6.334795192904015E-5	0.0	0.47662999031409814	9.502192789356023E-4
52	0.0	6.334795192904015E-5	0.0	0.5150821971350256	9.502192789356023E-4
53	0.0	1.266959038580803E-4	0.0	0.5574619769755534	0.001203611086651763
54	0.0	1.266959038580803E-4	0.0	0.5946472247579	0.001203611086651763
55	0.0	1.266959038580803E-4	0.0	0.6321492122998917	0.001266959038580803
56	0.0	1.266959038580803E-4	0.0	0.6762393868425037	0.001266959038580803
57	0.0	1.266959038580803E-4	0.0	0.7203295613851156	0.001266959038580803
58	0.0	1.266959038580803E-4	0.0	0.7654333031585923	0.001266959038580803
59	0.0	1.266959038580803E-4	0.0	0.8131976589130885	0.001266959038580803
60	0.0	1.266959038580803E-4	0.0	0.8642561081678949	0.001266959038580803
61	0.0	1.266959038580803E-4	0.0	0.9155679492304174	0.001266959038580803
62	0.0	1.266959038580803E-4	0.0	0.9711241030721857	0.001266959038580803
63	0.0	1.266959038580803E-4	0.0	1.0230694236539986	0.001266959038580803
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2790	0.0	40.024452	1
GGTATCA	1305	0.0	38.205353	1
TATCTCG	905	0.0	30.265226	36
TATCAAC	3755	0.0	29.444218	2
TCAACGC	3695	0.0	29.268442	4
ATCAACG	3750	0.0	29.029404	3
CAACGCA	3785	0.0	28.742735	5
AACGCAG	3885	0.0	28.091572	6
ATCTCGT	1105	0.0	26.23176	37
CCGTCTT	1230	0.0	25.938572	47
TCTCGTA	1130	0.0	25.782211	38
ACGGACT	1265	0.0	25.753412	27
CTCGTAT	1200	0.0	25.723385	39
TATGCCG	1260	0.0	25.445305	43
TCGTATG	1310	0.0	24.887194	40
CCTAATA	140	1.5601472E-8	24.374565	2
ACGCAGA	4490	0.0	24.14301	7
CCTATCT	1135	0.0	24.131405	34
ACTCCTA	1340	0.0	23.80468	31
CGGACTC	1390	0.0	23.687557	28
>>END_MODULE