FastQCFastQC Report
Mon 6 Feb 2017
SRR3203942_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203942_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181641
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40950.034.4546551
TCAACGC45300.030.2304694
CAACGCA46550.029.2704895
ATCAACG46650.029.2077433
AACGCAG48400.028.294226
GGTATCA18700.028.0401611
TATCAAC52450.026.4382742
ACGCAGA56800.023.3203727
GTACATG91700.022.4962621
CGCAGAG60550.021.705188
TACATGG89550.021.2245562
ACATGGG91650.020.0607553
AGAGTAC62950.019.2337511
TATACCG2707.2759576E-1219.163735
CATGGGG72600.018.5777324
GTATACG1804.205276E-617.2483881
CAGAGTA72100.017.17559210
GCGATAC3257.2759576E-1216.98201414
GCAGAGT76450.016.5592699
TCGCGTC9200.016.49747317