FastQCFastQC Report
Mon 6 Feb 2017
SRR3203942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4181641
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63250.041.0450481
GGTATCA25000.035.798841
TCAACGC85000.029.3445574
ATCAACG85700.029.1956843
CAACGCA85950.028.847955
AACGCAG88250.028.0574596
TATCAAC90550.027.969852
ACGCAGA102550.024.0337337
CGCAGAG108100.022.6421348
AGAGTAC109400.021.85223811
GTACATG88200.021.6883811
TATGCCG21950.020.72000143
TACATGG89850.020.4760132
CTCGTAT20750.020.3804839
CAGAGTA121100.020.01135610
TCGTATG22500.019.87278440
GAGTACT81600.019.59594312
GCAGAGT125500.019.4734139
ACATGGG91700.018.7238083
AGTACTT85400.018.68524613
Produced by FastQC (version 0.11.5)