FastQCFastQC Report
Mon 6 Feb 2017
SRR3203941_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203941_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285044
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33800.036.9444161
ATCAACG38000.031.316933
TCAACGC38300.031.0716274
CAACGCA38900.030.3263555
TATCAAC42400.028.9619522
AACGCAG41850.027.776546
ACGCAGA47800.024.102517
CGCAGAG52050.022.0019448
GTACATG75800.021.843841
GGTATCA16650.021.5464651
TACATGG75150.021.34362
CGAATCG2600.021.22740637
AGAGTAC52250.019.87119111
ACATGGG79200.019.8601883
CATGGGG62500.018.599174
CAGAGTA58050.018.59883710
GCAGAGT63850.017.0174059
ATGGGGG32800.016.7211825
TATACTG13050.016.3879595
ACTCGAC4500.016.0974546
Produced by FastQC (version 0.11.5)