##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203941_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3285044
Sequences flagged as poor quality	0
Sequence length	75
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09612534870157	35.0	31.0	35.0	15.0	35.0
2	31.15558847918019	35.0	31.0	35.0	15.0	35.0
3	31.10024188412697	35.0	31.0	35.0	15.0	35.0
4	31.41453904422589	35.0	31.0	35.0	15.0	35.0
5	31.36202802763068	35.0	31.0	35.0	15.0	35.0
6	34.73715420554489	39.0	34.0	40.0	16.0	40.0
7	34.29541430799709	39.0	34.0	40.0	16.0	40.0
8	34.37420046733012	39.0	34.0	40.0	16.0	40.0
9	34.34311808304546	39.0	34.0	40.0	16.0	40.0
10	34.51796840468499	39.0	34.0	40.0	16.0	40.0
11	34.61351385247808	39.0	34.0	40.0	16.0	40.0
12	34.26073105870119	39.0	34.0	40.0	16.0	40.0
13	34.32556337144951	39.0	34.0	40.0	16.0	40.0
14	34.27316102919778	39.0	34.0	40.0	16.0	40.0
15	34.359400361151934	39.0	34.0	40.0	16.0	40.0
16	34.47457263890529	39.0	34.0	40.0	16.0	40.0
17	34.51464851003518	39.0	34.0	40.0	16.0	40.0
18	34.554206884291354	39.0	34.0	40.0	16.0	40.0
19	34.595140278181965	39.0	34.0	40.0	16.0	40.0
20	34.5706273645041	39.0	34.0	40.0	16.0	40.0
21	34.58604633606125	39.0	34.0	40.0	16.0	40.0
22	34.64063890772848	39.0	34.0	40.0	16.0	40.0
23	34.64191742941647	39.0	34.0	40.0	16.0	40.0
24	34.66143101888437	39.0	34.0	40.0	16.0	40.0
25	34.67645851927706	39.0	34.0	40.0	16.0	40.0
26	34.68985894861682	39.0	34.0	40.0	16.0	40.0
27	34.69379253367687	39.0	34.0	40.0	16.0	40.0
28	34.56551662626132	39.0	34.0	40.0	16.0	40.0
29	34.650376068022226	39.0	34.0	40.0	16.0	40.0
30	34.66400328275664	39.0	34.0	40.0	16.0	40.0
31	34.65944078679007	39.0	34.0	40.0	16.0	40.0
32	34.594142422445486	39.0	34.0	40.0	16.0	40.0
33	34.49258153011041	39.0	34.0	40.0	16.0	40.0
34	34.513368161887634	39.0	34.0	40.0	16.0	40.0
35	34.50847659879137	39.0	34.0	40.0	16.0	40.0
36	34.50846290034471	39.0	34.0	40.0	16.0	40.0
37	34.476774740307896	39.0	34.0	40.0	16.0	40.0
38	34.483719243943156	39.0	34.0	40.0	16.0	40.0
39	34.44517425032968	39.0	34.0	40.0	16.0	40.0
40	34.37842841678833	39.0	34.0	40.0	16.0	40.0
41	34.34476554956341	39.0	34.0	40.0	16.0	40.0
42	34.34180759831527	39.0	34.0	40.0	16.0	40.0
43	34.23574844050795	39.0	34.0	40.0	16.0	40.0
44	34.10343544865761	39.0	34.0	40.0	16.0	40.0
45	34.106695983371914	39.0	34.0	40.0	16.0	40.0
46	34.053714957851405	39.0	34.0	40.0	16.0	40.0
47	33.97509805043707	39.0	31.0	40.0	16.0	40.0
48	33.993497195166945	39.0	34.0	40.0	16.0	40.0
49	33.93547544568657	39.0	31.0	40.0	16.0	40.0
50	33.834065540674644	39.0	31.0	40.0	16.0	40.0
51	33.875961783160285	39.0	31.0	40.0	16.0	40.0
52	33.89089552529585	39.0	31.0	40.0	16.0	40.0
53	33.815939756058064	38.0	31.0	40.0	16.0	40.0
54	33.85142786519754	38.0	31.0	40.0	16.0	40.0
55	33.834749245367796	38.0	31.0	40.0	16.0	40.0
56	33.77327183441074	38.0	31.0	40.0	16.0	40.0
57	33.65437083947734	38.0	31.0	40.0	16.0	40.0
58	33.66606352913386	38.0	31.0	39.0	16.0	40.0
59	33.592541226236236	38.0	31.0	39.0	16.0	40.0
60	33.54988548098595	38.0	31.0	39.0	16.0	40.0
61	33.513639999951295	38.0	31.0	39.0	16.0	40.0
62	33.46477703190581	38.0	31.0	39.0	16.0	40.0
63	33.432388120220004	38.0	31.0	39.0	16.0	40.0
64	33.39931793912045	38.0	31.0	39.0	16.0	40.0
65	33.31333096299471	38.0	31.0	39.0	16.0	40.0
66	33.26837113901671	38.0	31.0	39.0	16.0	40.0
67	33.252973780564275	38.0	31.0	39.0	16.0	40.0
68	33.20109715425425	38.0	31.0	39.0	16.0	40.0
69	33.16205871215119	38.0	31.0	39.0	16.0	40.0
70	33.05972492301473	38.0	31.0	39.0	16.0	40.0
71	32.948426261566055	38.0	30.0	39.0	16.0	40.0
72	32.90767338276139	38.0	30.0	39.0	16.0	40.0
73	32.8429835338583	38.0	30.0	39.0	16.0	40.0
74	32.78190246462452	38.0	30.0	39.0	16.0	40.0
75	32.67854159639871	38.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9200.0
3	2456.0
4	2626.0
5	2711.0
6	2458.0
7	2702.0
8	2775.0
9	3105.0
10	3444.0
11	3770.0
12	4277.0
13	4687.0
14	5397.0
15	6028.0
16	6686.0
17	7696.0
18	9797.0
19	18533.0
20	35085.0
21	48289.0
22	56013.0
23	62667.0
24	67839.0
25	71794.0
26	76568.0
27	81629.0
28	85588.0
29	91922.0
30	98848.0
31	107018.0
32	116837.0
33	128958.0
34	145101.0
35	169881.0
36	212858.0
37	295404.0
38	520140.0
39	714257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63653768233696	17.774400054065225	12.830689078976329	24.758373184621487
2	15.247923619896719	19.668138387187508	40.078001999364396	25.005935993551383
3	18.529980115943655	25.019238707304986	29.238360277670555	27.212420899080804
4	12.206198760199255	16.450799441346906	38.248620109806744	33.0943816886471
5	13.58039648784004	38.406334892318036	34.47086249073072	13.542406129111209
6	32.13789526106804	37.65340738206247	17.387894956658116	12.820802400211381
7	28.519191828176428	32.030986495158054	21.998031076600498	17.451790600065024
8	25.987170948090803	36.31394282694539	19.863052062620774	17.83583416234303
9	26.764755662329026	16.173481999023455	18.89667231245609	38.16509002619143
10	13.837105378192804	29.760879915154863	34.256892753947895	22.14512195270444
11	35.263424173313965	23.408636231356414	23.63213399881402	17.6958055965156
12	23.52361186029776	24.891325656520888	30.549332063741	21.03573041944035
13	28.02011175497193	22.356291118170716	25.75889394479952	23.864703182057838
14	21.967011705170464	20.968516707843182	27.29482466597099	29.769646921015365
15	23.46321693103654	29.420671382179357	23.576731392334473	23.539380294449632
16	23.63953116000882	28.43103471369029	24.95248161059639	22.976952515704507
17	22.52536039091105	28.12464612346136	26.659977765899022	22.690015719728564
18	22.535010185556114	27.960569173502698	27.00386965897565	22.50055098196554
19	23.528908593005145	27.4318091325413	26.951906884656644	22.08737538979691
20	23.657552227610953	26.973611312359896	26.90043116621878	22.468405293810374
21	23.712498219201937	26.821132380570855	26.96700561697195	22.499363783255262
22	23.45030995018636	26.60828896051316	26.880766285017792	23.06063480428268
23	23.432897702435646	26.6942238825416	26.64841018872198	23.224468226300772
24	22.98346688811474	26.7211641609671	26.98536153549237	23.31000741542579
25	23.33606490506672	26.697907242642714	26.87802659568639	23.088001256604173
26	23.06985842503175	27.09936305267144	26.774709866899805	23.056068655397006
27	23.24605089003374	27.051083638453548	26.6195521277645	23.083313343748213
28	23.427245438557794	27.03526670314764	26.482781020305442	23.054706837989126
29	23.407022858750143	27.02097749680065	26.571455359502032	23.00054428494717
30	23.4330499073985	26.868650769974465	26.628532220573	23.069767102054037
31	23.34766292323634	26.639247449958052	26.68247365940913	23.33061596739648
32	23.117924752301644	26.9156212215118	26.679125150226294	23.287328875960263
33	23.289554555350275	26.815308623024336	26.71230331681959	23.182833504805796
34	23.540658816137622	26.873186477867574	26.63736010841864	22.948794597576168
35	23.628694166653474	26.743416526536627	26.705821900711225	22.92206740609867
36	23.572956709255642	26.834343771346745	26.501167107655178	23.09153241174243
37	23.472653638733608	26.64332654296259	26.7366890671784	23.147330751125402
38	23.42058415616138	26.85497039280222	26.56081621619647	23.163629234839934
39	23.52615635824663	26.917568255783124	26.531362089817666	23.02491329615258
40	23.628244744437136	26.633228240847988	26.788812201240592	22.949714813474284
41	23.252692672752058	26.659058406234838	26.774339076768346	23.313909844244765
42	23.44378041845814	26.722702991648127	26.7152144345287	23.118302155365033
43	23.176589379912993	26.495301882260758	26.827961555403146	23.5001471824231
44	23.269760972754224	26.87162312105577	26.70160909071261	23.157006815477395
45	23.447676903463368	26.958805629933774	26.457528922268775	23.135988544334086
46	23.398991610145266	26.80924031647722	26.418072966561436	23.373695106816083
47	23.352920100540604	26.882860822741634	26.57872458821671	23.185494488501057
48	23.28983713430408	27.088636417600043	26.474092537133515	23.14743391096237
49	23.446725050478793	26.668265833963208	26.540535390252124	23.34447372530588
50	23.52195548720515	26.866488099206308	26.516537276794477	23.095019136794058
51	23.461282638640498	26.897177342638184	26.34348292630954	23.298057092411778
52	23.499636838295327	26.88973460885384	26.304018401004797	23.306610151846037
53	23.359346271208317	26.89438032871522	26.552922467726344	23.193350932350125
54	23.239635135332595	26.882589170469878	26.680850571577473	23.19692512262005
55	23.17032223223747	26.825735595622636	26.535476048488416	23.46846612365148
56	23.213938359865267	27.098889357343225	26.546688239242506	23.140484043549
57	23.442244532852204	26.831892910599624	26.364195649904065	23.361666906644107
58	23.319075695969254	26.59512581799275	26.67052863861227	23.415269847425726
59	23.340005022792827	26.90262631182411	26.47888524440453	23.27848342097853
60	23.185410589724434	27.01045188401191	26.63951867877763	23.164618847486025
61	23.495381363258886	26.629978154931944	26.731105964409828	23.143534517399342
62	23.220270205684724	26.953353430874294	26.58379246868854	23.242583894752446
63	23.15045040876513	26.80026520418774	26.75043287460396	23.298851512443168
64	23.22543545807916	26.717623711981986	26.669130398914575	23.387810431024274
65	23.252135477232738	26.811061254558627	26.572673197401507	23.36413007080713
66	23.24127620699596	27.01298922693871	26.558523059538448	23.187211506526882
67	23.234421247554394	26.799779967616153	26.53831647220021	23.427482312629245
68	23.23389422893978	26.89187728791373	26.77345753708228	23.100770946064216
69	23.421190181699625	26.853686550720912	26.470542572178925	23.254580695400538
70	23.16753599963956	26.566428018681883	26.743146049765986	23.522889931912566
71	23.296119079119205	26.702815453058225	26.614594717349654	23.386470750472917
72	23.11580122720826	26.90327021138703	26.558157842105533	23.42277071929917
73	23.206696080944262	26.728403367279284	26.626303718752908	23.438596833023546
74	23.155673141289487	26.93834572458454	26.586045146711747	23.31993598741423
75	23.26459739414494	26.78640737391046	26.658644311225583	23.290350920719018
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	314.0
1	377.5
2	468.0
3	462.0
4	429.0
5	401.5
6	369.5
7	412.0
8	459.0
9	556.5
10	810.0
11	916.0
12	866.0
13	958.0
14	1125.5
15	1341.0
16	1481.0
17	1760.0
18	2367.0
19	3478.5
20	4262.0
21	5305.0
22	8486.0
23	12532.0
24	14440.0
25	17485.5
26	24531.0
27	32117.5
28	35704.0
29	40937.0
30	51159.5
31	60953.0
32	65757.0
33	71055.5
34	81395.5
35	92365.0
36	98293.0
37	102444.5
38	111119.0
39	119599.0
40	123556.0
41	129491.5
42	137914.5
43	144880.5
44	149359.0
45	153278.5
46	159173.5
47	163824.5
48	166500.0
49	168115.0
50	167645.5
51	163450.0
52	161339.0
53	157055.5
54	148117.0
55	137039.5
56	130617.0
57	125204.0
58	109815.5
59	91043.5
60	82247.0
61	74946.5
62	59570.0
63	45101.0
64	38708.0
65	32246.0
66	21855.5
67	15466.5
68	13006.0
69	10914.5
70	7244.0
71	4677.0
72	3689.0
73	3303.5
74	2480.0
75	1645.5
76	1249.0
77	980.5
78	541.5
79	281.5
80	192.0
81	147.5
82	78.5
83	39.5
84	25.0
85	16.0
86	6.5
87	3.5
88	1.0
89	1.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0037442420862551615
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.09034886595126275
29	0.0
30	0.0
31	0.0
32	0.0
33	0.05229762523728754
34	0.0
35	0.0
36	0.0
37	0.0
38	9.741117622777655E-4
39	3.348509182829819E-4
40	3.044099257118017E-5
41	5.479378662812431E-4
42	9.741117622777655E-4
43	1.5220496285590088E-4
44	6.392608439947836E-4
45	9.741117622777655E-4
46	1.5220496285590088E-4
47	9.132297771354052E-5
48	1.8264595542708104E-4
49	3.044099257118017E-5
50	3.348509182829819E-4
51	5.783788588524233E-4
52	1.8264595542708104E-4
53	3.6529191085416207E-4
54	0.003044099257118017
55	0.0016133726062725492
56	2.7396893314062155E-4
57	3.957329034253422E-4
58	6.392608439947836E-4
59	5.783788588524233E-4
60	0.002617925361121495
61	0.002800571316548576
62	0.001978664517126711
63	8.523477919930449E-4
64	0.0016742545914149095
65	0.001948223524555531
66	0.0027701303239773957
67	0.0022221924576961527
68	0.0036224781159704404
69	0.0035615961308280803
70	0.0045661488856770256
71	0.0031049812422603773
72	8.219067994218647E-4
73	5.479378662812431E-4
74	0.001978664517126711
75	0.00173513657655727
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	3285044.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.67956687251377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.83234278390178	45.85320939143767
2	11.939352018460427	14.250707144003826
3	4.71331332014705	8.438654924424773
4	2.228092151158965	5.31886298132858
5	1.2406623767072376	3.7021096638455724
6	0.7578234356423229	2.7135944642984486
7	0.5306965587557483	2.217021853709363
8	0.35608920099020264	1.7000999426459835
9	0.252090329391605	1.354017750375628
>10	1.091048352614971	10.888234820298042
>50	0.042783194964925775	1.728317474334412
>100	0.014877964063834707	1.5033480729539102
>500	7.765436262259903E-4	0.2976198015424114
>1k	5.1769574772195556E-5	0.034201714801337824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	3.044099257118017E-5	0.0	0.0	0.0
10	0.0	3.044099257118017E-5	0.0	0.0	0.0
11	0.0	3.044099257118017E-5	0.0	0.0	0.0
12	0.0	3.044099257118017E-5	0.0	0.0	0.0
13	0.0	3.044099257118017E-5	0.0	0.0	0.0
14	0.0	3.044099257118017E-5	0.0	0.0	0.0
15	0.0	3.044099257118017E-5	0.0	0.0	0.0
16	0.0	3.044099257118017E-5	0.0	0.0	0.0
17	0.0	3.044099257118017E-5	0.0	0.0	0.0
18	0.0	3.044099257118017E-5	0.0	0.0	0.0
19	0.0	3.044099257118017E-5	0.0	0.0	0.0
20	0.0	3.044099257118017E-5	0.0	0.0	0.0
21	0.0	3.044099257118017E-5	0.0	3.044099257118017E-5	0.0
22	0.0	3.044099257118017E-5	0.0	3.044099257118017E-5	0.0
23	0.0	3.044099257118017E-5	0.0	6.088198514236034E-5	0.0
24	0.0	3.044099257118017E-5	0.0	9.132297771354052E-5	0.0
25	0.0	3.044099257118017E-5	0.0	9.132297771354052E-5	0.0
26	0.0	3.044099257118017E-5	0.0	1.5220496285590085E-4	0.0
27	0.0	3.044099257118017E-5	0.0	1.5220496285590085E-4	0.0
28	0.0	3.044099257118017E-5	0.0	6.088198514236034E-4	3.044099257118017E-5
29	0.0	3.044099257118017E-5	0.0	0.0010958757325624862	3.044099257118017E-5
30	0.0	3.044099257118017E-5	0.0	0.0018264595542708104	3.044099257118017E-5
31	0.0	3.044099257118017E-5	0.0	0.004900999803960008	3.044099257118017E-5
32	0.0	3.044099257118017E-5	0.0	0.008462595934788089	3.044099257118017E-5
33	0.0	3.044099257118017E-5	0.0	0.011719782139904367	3.044099257118017E-5
34	0.0	3.044099257118017E-5	0.0	0.015311819263303626	6.088198514236034E-5
35	0.0	3.044099257118017E-5	0.0	0.01920826631241469	6.088198514236034E-5
36	0.0	6.088198514236034E-5	0.0	0.024231030086659416	6.088198514236034E-5
37	0.0	6.088198514236034E-5	0.0	0.03257186205116278	6.088198514236034E-5
38	0.0	6.088198514236034E-5	0.0	0.04380458830992827	6.088198514236034E-5
39	0.0	9.132297771354052E-5	0.0	0.05601142633097152	9.132297771354052E-5
40	0.0	9.132297771354052E-5	0.0	0.068766202218296	1.2176397028472069E-4
41	0.0	9.132297771354052E-5	0.0	0.0819775929941882	1.2176397028472069E-4
42	0.0	9.132297771354052E-5	0.0	0.095797803621504	1.2176397028472069E-4
43	3.044099257118017E-5	9.132297771354052E-5	0.0	0.11159667876594652	1.2176397028472069E-4
44	3.044099257118017E-5	9.132297771354052E-5	0.0	0.12754775887324493	1.2176397028472069E-4
45	3.044099257118017E-5	9.132297771354052E-5	0.0	0.14413809982453812	1.2176397028472069E-4
46	3.044099257118017E-5	9.132297771354052E-5	0.0	0.16045447184269068	1.2176397028472069E-4
47	3.044099257118017E-5	9.132297771354052E-5	0.0	0.1782624524968311	1.2176397028472069E-4
48	3.044099257118017E-5	9.132297771354052E-5	0.0	0.19740983682410343	1.5220496285590085E-4
49	3.044099257118017E-5	9.132297771354052E-5	0.0	0.2175617739062247	1.5220496285590085E-4
50	3.044099257118017E-5	9.132297771354052E-5	0.0	0.2382920898471984	2.1308694799826122E-4
51	3.044099257118017E-5	9.132297771354052E-5	0.0	0.25972254861730926	2.1308694799826122E-4
52	3.044099257118017E-5	9.132297771354052E-5	0.0	0.28319255388968917	2.4352794056944137E-4
53	3.044099257118017E-5	1.2176397028472069E-4	0.0	0.30788019886491624	2.4352794056944137E-4
54	3.044099257118017E-5	1.2176397028472069E-4	0.0	0.33360283758756354	2.4352794056944137E-4
55	3.044099257118017E-5	1.2176397028472069E-4	0.0	0.3591732713473549	2.4352794056944137E-4
56	3.044099257118017E-5	1.2176397028472069E-4	0.0	0.3865397236688458	2.4352794056944137E-4
57	3.044099257118017E-5	1.2176397028472069E-4	0.0	0.4150020517228993	2.7396893314062155E-4
58	3.044099257118017E-5	1.5220496285590085E-4	0.0	0.4472390628557791	2.7396893314062155E-4
59	3.044099257118017E-5	1.5220496285590085E-4	0.0	0.47847152123381	2.7396893314062155E-4
60	3.044099257118017E-5	1.5220496285590085E-4	0.0	0.513448221698096	2.7396893314062155E-4
61	3.044099257118017E-5	1.5220496285590085E-4	0.0	0.5490946239989479	2.7396893314062155E-4
62	3.044099257118017E-5	1.5220496285590085E-4	0.0	0.5871458647129232	2.7396893314062155E-4
63	3.044099257118017E-5	1.5220496285590085E-4	0.0	0.6244360806126189	2.7396893314062155E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3380	0.0	36.944416	1
ATCAACG	3800	0.0	31.31693	3
TCAACGC	3830	0.0	31.071627	4
CAACGCA	3890	0.0	30.326355	5
TATCAAC	4240	0.0	28.961952	2
AACGCAG	4185	0.0	27.77654	6
ACGCAGA	4780	0.0	24.10251	7
CGCAGAG	5205	0.0	22.001944	8
GTACATG	7580	0.0	21.84384	1
GGTATCA	1665	0.0	21.546465	1
TACATGG	7515	0.0	21.3436	2
CGAATCG	260	0.0	21.227406	37
AGAGTAC	5225	0.0	19.871191	11
ACATGGG	7920	0.0	19.860188	3
CATGGGG	6250	0.0	18.59917	4
CAGAGTA	5805	0.0	18.598837	10
GCAGAGT	6385	0.0	17.017405	9
ATGGGGG	3280	0.0	16.721182	5
TATACTG	1305	0.0	16.387959	5
ACTCGAC	450	0.0	16.09745	46
>>END_MODULE