FastQCFastQC Report
Mon 6 Feb 2017
SRR3203941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285044
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53850.042.6411171
GGTATCA20700.035.047521
ATCAACG70400.031.8721313
TCAACGC70050.031.7770654
TATCAAC71800.031.4393042
CAACGCA71300.031.2085555
AACGCAG74050.030.0500166
ACGCAGA88350.025.1793337
CGCAGAG91300.024.2164178
AGAGTAC92950.023.02184911
GAGTACT68350.022.41215712
CAGAGTA100350.021.72037310
AGTACTT72900.021.3900513
GCAGAGT103150.021.1314189
GTACATG76650.020.5816671
GTACTTT74400.020.49847814
CTCGTAT16700.019.8899639
TACATGG77200.019.803582
TATGCCG17750.019.18959443
TCGTATG18300.019.0957240
Produced by FastQC (version 0.11.5)