FastQCFastQC Report
Mon 6 Feb 2017
SRR3203940_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203940_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053257
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39300.039.8493351
TCAACGC48700.031.7311174
CAACGCA49500.031.2879755
ATCAACG50950.030.3298443
TATCAAC52550.029.4063842
AACGCAG52100.029.196936
GGTATCA16800.027.5139711
ACGCAGA60000.024.605317
CGCAGAG62250.023.1618488
GTACATG87650.022.196521
TACATGG90650.021.1945272
AGAGTAC63800.021.13938711
ACATGGG95400.019.741523
CAGAGTA73150.019.00321410
CATGGGG71350.018.5640854
GCAGAGT76450.018.4085259
GAGTACT32800.016.93122312
GTATAGG8650.015.5527161
GAGTACA63550.015.3070431
TCTATAC9950.014.9067063
Produced by FastQC (version 0.11.5)