FastQCFastQC Report
Mon 6 Feb 2017
SRR3203940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053257
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75300.041.3808861
GGTATCA29650.041.3684961
TATCAAC100600.030.1738032
TCAACGC99150.030.08814
ATCAACG100150.029.8335443
CAACGCA99600.029.80335
AACGCAG104100.028.5805266
ACGCAGA118100.024.7763987
CGCAGAG120250.024.2199698
AGAGTAC124100.022.29598811
GTACATG90950.021.4878671
GAGTACT92400.021.41673712
CAGAGTA134400.021.26061810
GCAGAGT136750.021.0453919
AGTACTT98550.020.11646513
TACATGG92100.020.0198292
ACATGGG93400.018.9744073
GTACTTT105200.018.84484514
CATGGGG74700.017.5006524
TACTTTT118150.016.77974115
Produced by FastQC (version 0.11.5)