FastQCFastQC Report
Mon 6 Feb 2017
SRR3203939_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203939_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588582
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32950.036.5368081
TCAACGC40050.029.2832224
ATCAACG39750.029.2438953
CAACGCA40750.028.8648455
TATCAAC44200.026.9239792
AACGCAG43400.026.8639226
GGTATCA16550.025.0117361
ACGCAGA52600.021.509517
GTACATG78500.020.697211
CGCAGAG55250.020.352978
TACATGG79500.020.0889072
AGAGTAC55850.018.77555811
ACATGGG81750.018.5233363
CGAATCG3750.018.39726437
CGTGCCG4500.017.6302210
CAGAGTA64400.016.65777410
CATGGGG62500.016.1155554
TAACTCG3251.2369128E-1015.92093251
GCAGAGT68650.015.8275049
TATAGCG1605.770016E-415.0910855
Produced by FastQC (version 0.11.5)