FastQCFastQC Report
Mon 6 Feb 2017
SRR3203939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3588582
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56900.041.448021
GGTATCA22800.034.529461
ATCAACG76200.030.3465793
TCAACGC75250.030.0335714
TATCAAC80000.029.2463722
CAACGCA77500.029.019165
AACGCAG79400.028.4108376
TATGCCG15000.026.85589243
CTCGTAT14700.025.43313239
TCGTATG15550.025.37825440
ACGCAGA90850.024.8208247
CGCAGAG93900.023.9782248
AATCTCG13400.023.81048636
ACGTAGA16900.023.38236427
AGACGTA17850.023.30307825
CGAGACG18150.023.10855923
GACGTAG17450.023.04267126
TCTCGTA13900.022.91250638
AGAGTAC95800.022.9053111
ATGCCGT17600.022.88757944
Produced by FastQC (version 0.11.5)