FastQCFastQC Report
Mon 6 Feb 2017
SRR3203938_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203938_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696823
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43650.037.855671
GGTATCA20450.029.689241
TCAACGC54150.028.9198444
ATCAACG54450.028.7605063
CAACGCA53750.028.7500175
TATCAAC57900.027.5829682
AACGCAG56600.026.9976436
ACGCAGA65500.022.7499797
CGCAGAG68700.021.2384198
GTACATG94700.021.0915371
AGAGTAC68200.020.12969811
TACATGG99450.019.6660352
ACATGGG99600.018.528193
CAGAGTA77200.018.2297810
GAGTACT30350.017.5025212
GCAGAGT81200.017.3317629
CGTGCCG5250.017.08254610
GTATTAC7400.016.782261
CATGGGG68500.016.4662914
GTATAAT15050.016.2742711
Produced by FastQC (version 0.11.5)