FastQCFastQC Report
Mon 6 Feb 2017
SRR3203938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696823
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68250.042.8401261
GGTATCA25900.038.9046821
TATCAAC93800.030.387742
ATCAACG94250.030.0635973
TCAACGC95350.029.5953224
CAACGCA95800.029.4071485
AACGCAG99700.028.0857726
ACGCAGA114700.024.1318157
CGCAGAG117350.023.441638
AGAGTAC122300.021.80548511
GAGTACT83950.021.5750612
GTACATG90650.020.7617231
CAGAGTA131350.020.497210
AGTACTT89050.020.4171613
GCAGAGT136550.019.9173819
CGAATCG4450.019.61586437
GTACTTT93200.019.3983414
TACATGG94300.019.1857572
AGGCGAA16250.018.57213631
ACATGGG94150.018.3111343
Produced by FastQC (version 0.11.5)