FastQCFastQC Report
Mon 6 Feb 2017
SRR3203937_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203937_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4687526
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39200.035.7253531
ATCAACG47850.027.8254343
TCAACGC48100.027.7525234
CAACGCA48500.027.5236365
TATCAAC52200.025.9692022
AACGCAG51200.025.8027136
GGTATCA19550.023.8190021
GTACATG97950.022.256111
TACATGG95750.021.686742
ACGCAGA60650.021.384237
ACATGGG97650.019.5339243
CGCAGAG66100.019.1514288
AGAGTAC64300.017.48812311
CATGGGG80850.016.4681134
CAGAGTA75900.015.31527110
GCAGAGT80900.015.0509119
ATGGGGG39900.014.7829055
TATACCG3605.820766E-1014.372275
CGAATCG3951.5643309E-1013.97232537
GTATAGA12850.013.9584371
Produced by FastQC (version 0.11.5)