FastQCFastQC Report
Mon 6 Feb 2017
SRR3203937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4687526
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64000.041.8186261
GGTATCA26550.032.8294531
TATCAAC90700.029.1107792
TCAACGC90250.028.8663564
ATCAACG91000.028.7539043
CAACGCA90800.028.4900995
AACGCAG94400.027.3677526
ACGCAGA110550.023.3283317
CGCAGAG116050.022.163938
GTACATG99000.021.5790731
TACATGG97650.021.2673282
AGAGTAC117950.020.89166511
AATCTCG13150.019.51505736
CAGAGTA130050.019.2752610
ACATGGG101000.019.2442233
GAGTACT87050.019.23882512
GCAGAGT135800.018.7614889
GGCGAAT14400.018.54432132
TATGCCG17900.018.29106543
CTCGTAT17550.018.1685539
Produced by FastQC (version 0.11.5)