##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2079283_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 349747 Sequences flagged as poor quality 0 Sequence length 75 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.608682847887188 31.0 30.0 34.0 16.0 34.0 2 28.77818251478926 31.0 30.0 34.0 16.0 34.0 3 29.048769539123995 31.0 30.0 34.0 18.0 34.0 4 32.109779354790746 37.0 33.0 37.0 18.0 37.0 5 32.455506408918446 37.0 33.0 37.0 22.0 37.0 6 32.2549128369936 37.0 33.0 37.0 17.0 37.0 7 32.10286578583948 37.0 33.0 37.0 17.0 37.0 8 31.9772378319185 37.0 33.0 37.0 15.0 37.0 9 33.16218294938913 39.0 34.0 39.0 11.0 39.0 10 33.26829965660892 39.0 34.0 39.0 13.0 39.0 11 33.19512104464084 39.0 34.0 39.0 11.0 39.0 12 33.2258146603116 39.0 34.0 39.0 12.0 39.0 13 33.09155475243533 39.0 34.0 39.0 10.0 39.0 14 34.16892210655131 40.0 34.0 41.0 10.0 41.0 15 34.211667290927444 40.0 34.0 41.0 11.0 41.0 16 34.26735897663168 40.0 34.0 41.0 11.0 41.0 17 34.31850165977121 40.0 34.0 41.0 12.0 41.0 18 34.329967090496844 40.0 34.0 41.0 12.0 41.0 19 34.417161548204845 40.0 34.0 41.0 13.0 41.0 20 34.410402376575064 40.0 34.0 41.0 13.0 41.0 21 34.41009072272242 40.0 34.0 41.0 13.0 41.0 22 34.408489565314355 40.0 34.0 41.0 13.0 41.0 23 34.4328814829005 40.0 34.0 41.0 16.0 41.0 24 34.49043165488196 40.0 34.0 41.0 17.0 41.0 25 34.47164378822406 40.0 34.0 41.0 17.0 41.0 26 34.46686890809642 40.0 34.0 41.0 17.0 41.0 27 34.45654716123369 40.0 34.0 41.0 17.0 41.0 28 34.379517193857275 40.0 34.0 41.0 17.0 41.0 29 34.389861814397264 40.0 34.0 41.0 16.0 41.0 30 34.26672137287811 39.0 34.0 41.0 16.0 41.0 31 34.19275361904462 39.0 34.0 41.0 15.0 41.0 32 34.07150025589927 39.0 34.0 41.0 10.0 41.0 33 33.99183981563816 39.0 33.0 41.0 9.0 41.0 34 33.94660425965055 39.0 33.0 41.0 9.0 41.0 35 33.85886369289801 39.0 33.0 41.0 8.0 41.0 36 33.751363128204105 39.0 33.0 41.0 2.0 41.0 37 33.70711971796756 39.0 33.0 41.0 2.0 41.0 38 33.598226718170565 39.0 33.0 41.0 2.0 41.0 39 33.559187069510244 39.0 33.0 41.0 2.0 41.0 40 33.49947533502789 39.0 33.0 41.0 2.0 41.0 41 33.4127354916554 39.0 33.0 41.0 2.0 41.0 42 33.30607839381038 39.0 33.0 41.0 2.0 41.0 43 33.20888242072127 39.0 33.0 41.0 2.0 41.0 44 33.072452372715134 39.0 33.0 41.0 2.0 41.0 45 32.95530197542795 39.0 32.0 41.0 2.0 41.0 46 32.81415423148733 39.0 32.0 41.0 2.0 41.0 47 32.68355983039168 39.0 32.0 41.0 2.0 41.0 48 32.53305675245249 39.0 32.0 41.0 2.0 41.0 49 32.5062659579639 39.0 32.0 41.0 2.0 41.0 50 32.41238952728687 39.0 31.0 41.0 2.0 41.0 51 32.335399588845654 39.0 31.0 41.0 2.0 41.0 52 32.28917188710697 39.0 31.0 41.0 2.0 41.0 53 32.197554232059176 39.0 31.0 41.0 2.0 41.0 54 32.13255867813019 39.0 31.0 41.0 2.0 41.0 55 32.083368835186576 38.0 31.0 41.0 2.0 41.0 56 32.00542677992949 38.0 31.0 41.0 2.0 41.0 57 31.89587044349201 38.0 31.0 40.0 2.0 41.0 58 31.80319488087103 38.0 30.0 40.0 2.0 41.0 59 31.76267416160825 38.0 31.0 40.0 2.0 41.0 60 31.6406573894844 38.0 31.0 40.0 2.0 41.0 61 31.577620394170644 38.0 31.0 40.0 2.0 41.0 62 31.48637157716864 38.0 30.0 40.0 2.0 41.0 63 31.352183149533804 38.0 30.0 40.0 2.0 41.0 64 31.24216933955116 38.0 30.0 40.0 2.0 41.0 65 31.100615587839208 37.0 30.0 40.0 2.0 41.0 66 30.96376809522312 37.0 30.0 40.0 2.0 41.0 67 30.786722974035516 37.0 30.0 40.0 2.0 41.0 68 30.616925949329087 37.0 29.0 40.0 2.0 41.0 69 30.451300511512606 36.0 29.0 39.0 2.0 41.0 70 30.266764261022967 36.0 29.0 39.0 2.0 41.0 71 30.073864822285824 36.0 29.0 39.0 2.0 41.0 72 29.849945817976995 36.0 29.0 39.0 2.0 41.0 73 29.630964668746266 35.0 29.0 39.0 2.0 40.0 74 29.34952122534289 35.0 28.0 39.0 2.0 40.0 75 29.097736363714343 35.0 28.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.248167545728414 1101 2 1.5041940022929978 1101 3 1.654662541922196 1101 4 1.4531865579980732 1101 5 1.6788117037821166 1101 6 1.715910974449919 1101 7 1.7204643221950775 1101 8 1.6969200791796695 1101 9 1.6365348741468253 1101 10 1.6370239729359994 1101 11 1.2835889621954593 1101 12 1.757192598369251 1101 13 1.2796961844238766 1101 14 1.9875850315723795 1101 15 1.9358079787861584 1101 16 1.8749347079339032 1101 17 1.7233583780913264 1101 18 1.8288831015595477 1101 19 1.5762273494523669 1101 20 1.7047645567395122 1101 21 1.5034493409069611 1101 22 1.50191037747868 1101 23 1.442611112412429 1101 24 1.290731123483404 1101 25 1.3170915275011694 1101 26 1.2900295827076746 1101 27 1.4075884701775365 1101 28 1.4836278676584556 1101 29 1.5710720202224664 1101 30 1.4437734987919413 1101 31 1.5913012205041923 1101 32 1.7065471333683746 1101 33 1.5361136550409427 1101 34 1.5881598276644056 1101 35 1.3827534374062438 1101 36 1.4951681715808647 1101 37 1.4516270013188617 1101 38 0.8814660382650885 1101 39 1.0377700540478614 1101 40 0.7773495611906185 1101 41 0.6467147414681378 1101 42 0.8032271016902541 1101 43 0.7548592097500091 1101 44 0.8796257752825483 1101 45 0.7193485314763493 1101 46 0.6037333796848046 1101 47 0.8091980916270884 1101 48 0.8055491594493986 1101 49 0.9432840908966895 1101 50 0.9375396493837655 1101 51 1.0199154857540123 1101 52 0.8634010591431291 1101 53 0.8792908272060274 1101 54 0.6817162373705372 1101 55 0.566122394119553 1101 56 0.7052237659407901 1101 57 0.4520396036696077 1101 58 0.6765654281323208 1101 59 0.6229821977080832 1101 60 0.6707769317742631 1101 61 0.31472988017963033 1101 62 0.49971568192968974 1101 63 0.4901697599225905 1101 64 0.4894724778570385 1101 65 0.6644217964327481 1101 66 0.5951368252385514 1101 67 0.5186660006737256 1101 68 0.4093509061043932 1101 69 0.5191081833513564 1101 70 0.5040126074523279 1101 71 0.5121886225282566 1101 72 0.806732377013347 1101 73 0.6657080559499029 1101 74 0.7755000873642608 1101 75 0.8610840237992008 1102 1 0.5253935340607185 1102 2 0.30536647564957775 1102 3 0.46318843573961743 1102 4 0.7390899898658816 1102 5 0.817952270219557 1102 6 0.864771907213985 1102 7 1.0613086007858286 1102 8 0.7916671704028246 1102 9 0.8898428948749171 1102 10 0.8616760604111491 1102 11 0.8338733216127281 1102 12 0.8942799105616714 1102 13 0.8406827242016064 1102 14 0.8305966340884297 1102 15 0.5409231891632444 1102 16 0.5210946336256512 1102 17 0.7252283060644729 1102 18 0.6746771894909713 1102 19 0.6295712206513997 1102 20 0.7746281619932347 1102 21 0.5674342837091615 1102 22 0.6868621422995886 1102 23 1.0404380850031103 1102 24 1.0784922615728973 1102 25 0.6441089312752482 1102 26 0.5731260856571936 1102 27 0.48576286642301625 1102 28 0.7616808611727777 1102 29 0.983490949271129 1102 30 0.8787578549500736 1102 31 0.8155825815184343 1102 32 0.9415021573230078 1102 33 0.5671772733312039 1102 34 0.7017121204649825 1102 35 0.8264396806028032 1102 36 0.8950557314674441 1102 37 0.8086610266749261 1102 38 0.8709919972651861 1102 39 0.9645373193087536 1102 40 0.8342499620640638 1102 41 0.8933619076513679 1102 42 0.5293987602634118 1102 43 0.6404391725958902 1102 44 0.8075663286834569 1102 45 0.9235006678385034 1102 46 0.8013020659279988 1102 47 0.665767690427721 1102 48 0.6527854140529215 1102 49 0.7378853358857711 1102 50 0.49583332992347806 1102 51 0.5167011651204305 1102 52 0.5862687709053418 1102 53 0.4858074650836812 1102 54 0.3992511915797081 1102 55 0.36843556427892565 1102 56 0.20152872308318237 1102 57 0.4305170993512597 1102 58 0.3390373181039621 1102 59 0.45532421120090305 1102 60 0.2971824331919848 1102 61 0.28313257874235376 1102 62 0.3900662017615204 1102 63 0.3211796025064295 1102 64 0.4887310249352268 1102 65 0.32821770947905904 1102 66 0.22950276077288478 1102 67 0.16060942211230866 1102 68 0.2748057182153332 1102 69 -0.17280183196657362 1102 70 0.20203349849948893 1102 71 0.23565112619478157 1102 72 -0.01379935065343929 1102 73 0.2216144210380655 1102 74 0.14586804356150296 1102 75 -0.010119085414370232 1103 1 0.09649515017051158 1103 2 0.0532421249794055 1103 3 0.015483536105165285 1103 4 0.07435260770721897 1103 5 0.19170170907032258 1103 6 -0.02615671460349489 1103 7 0.14338870083071953 1103 8 0.25327253819606454 1103 9 0.35378104019653733 1103 10 -0.108943768999481 1103 11 -0.08813895953907291 1103 12 0.23792369302815786 1103 13 0.14735879679827946 1103 14 0.17283754347084823 1103 15 0.12142405493634811 1103 16 0.03503518387255866 1103 17 -0.02079154258556315 1103 18 0.14762979642998175 1103 19 0.09714881957399513 1103 20 -0.24387372988132228 1103 21 0.19517990674513896 1103 22 0.12089504167751386 1103 23 0.022000324469537702 1103 24 -0.1423400029047528 1103 25 -0.04716680142532681 1103 26 0.01639790634596494 1103 27 0.10255806298557957 1103 28 -0.1364144379999317 1103 29 -0.11500677406626636 1103 30 -0.049186575242963215 1103 31 0.1416383437194213 1103 32 0.1443776463244788 1103 33 0.16514591310546223 1103 34 0.29634993871675874 1103 35 0.19611277109212466 1103 36 0.1552686475195202 1103 37 0.12336153331251865 1103 38 -0.004440889286470906 1103 39 -0.23087087667661876 1103 40 -0.11553118973481702 1103 41 -0.21827071596179337 1103 42 -0.09285962490942268 1103 43 -0.17315506840969164 1103 44 -0.035961215727496665 1103 45 -0.14765305498583814 1103 46 -0.11414076098151327 1103 47 -0.028189533967839964 1103 48 -0.10597252220052411 1103 49 0.08461143092841894 1103 50 -0.1235391449049672 1103 51 -0.3354520446531808 1103 52 -0.06861242578548143 1103 53 -0.2729103890816482 1103 54 -0.2082890508367612 1103 55 -0.16983080524586214 1103 56 -0.40672097229294835 1103 57 -0.12573009490257547 1103 58 -0.14228042062495305 1103 59 -0.23664894983289742 1103 60 -0.5905596626202403 1103 61 -0.6598867247912814 1103 62 -0.41173989712901005 1103 63 -0.5621173897336753 1103 64 -0.7082403724866957 1103 65 -0.5139229946830639 1103 66 -0.46686740532728876 1103 67 -0.40819148213959977 1103 68 -0.3393600933667855 1103 69 -0.4406022872990931 1103 70 -0.1418427600780774 1103 71 -0.11251391581124892 1103 72 -0.19875413805804243 1103 73 -0.24330172723359667 1103 74 -0.40684391052045754 1103 75 -0.5302763675281419 1104 1 0.4198758465350778 1104 2 0.4122411009138638 1104 3 0.5275483082505836 1104 4 0.7333387828224289 1104 5 0.8212459999049386 1104 6 0.3783491737733584 1104 7 0.5786805595096709 1104 8 0.4209312683547921 1104 9 0.5065908200224456 1104 10 0.5219446078566321 1104 11 0.6233625765592379 1104 12 0.47323475293599415 1104 13 0.8406511675100106 1104 14 0.6676852137217395 1104 15 0.7059094620023956 1104 16 0.3706086605750656 1104 17 0.4392378992987176 1104 18 0.6407813581299351 1104 19 0.7463743700092209 1104 20 0.926889198946121 1104 21 1.1248714070011516 1104 22 0.8353087641557551 1104 23 1.0452978155089667 1104 24 1.0196863667829135 1104 25 1.0128370388204502 1104 26 1.0570085054242995 1104 27 1.029433383006058 1104 28 1.118385869219729 1104 29 1.0322026473367032 1104 30 0.7036616070407007 1104 31 0.9502980979436941 1104 32 0.9552135398216635 1104 33 0.9230184169457019 1104 34 1.0138038557674562 1104 35 0.6900003798990895 1104 36 0.9392548226347301 1104 37 1.0297235404809797 1104 38 0.981030180862021 1104 39 0.9812386983361776 1104 40 0.8869969720675357 1104 41 0.904521142717158 1104 42 0.8946595763194551 1104 43 0.9044819003563092 1104 44 1.1033116977389668 1104 45 0.7172342978047368 1104 46 0.7502985631352317 1104 47 0.4467208602973969 1104 48 0.3904112463606637 1104 49 0.13216647445120344 1104 50 0.21811472094244522 1104 51 0.1736365370160442 1104 52 -0.050403260398894645 1104 53 -0.02147029691575142 1104 54 0.031150273279983764 1104 55 0.042182763622541586 1104 56 -0.2293976235986328 1104 57 -0.13753885066294202 1104 58 -0.12957560751691233 1104 59 -0.2237521244360714 1104 60 0.03735252375968656 1104 61 -0.16308692960438975 1104 62 -0.22283180701124294 1104 63 -0.0036176457500616266 1104 64 -0.06634329107348336 1104 65 -0.03581239560472227 1104 66 0.08420786348991527 1104 67 0.2658036535490851 1104 68 0.15395147857381986 1104 69 0.29682090778539205 1104 70 0.08399569358295622 1104 71 -0.024924310076482215 1104 72 0.0697430457617223 1104 73 -0.006390564919207975 1104 74 -0.01990714656756154 1104 75 0.2157873805517383 1105 1 -0.43586779967099787 1105 2 -0.4309784605137885 1105 3 -0.09558225418633626 1105 4 -0.13254340820427046 1105 5 -0.27152627278062624 1105 6 -0.10731464859566131 1105 7 -0.04064231414845665 1105 8 -0.09968988954960167 1105 9 0.023668074816463047 1105 10 -0.443900882555333 1105 11 -0.2596886207983147 1105 12 -0.1776047736260722 1105 13 -0.0576881296732239 1105 14 -0.2266628916090241 1105 15 -0.43508971223376847 1105 16 -0.26410553338768494 1105 17 -0.28676907381807837 1105 18 -0.27780062186823073 1105 19 -0.5478842775731252 1105 20 -0.5994893678948756 1105 21 -0.42645210891773644 1105 22 -0.4632851435422012 1105 23 -0.5083309577729906 1105 24 -0.4806806393644436 1105 25 -0.4070982763460762 1105 26 -0.3998453343775168 1105 27 -0.367495234298282 1105 28 -0.3453149290186346 1105 29 -0.5525184279913091 1105 30 -0.5450882782698727 1105 31 -0.7039821112498146 1105 32 -0.6508512367633443 1105 33 -0.5012177515101186 1105 34 -0.3142652331397855 1105 35 -0.5362703788861225 1105 36 -0.40471022002973456 1105 37 -0.4039630362691824 1105 38 -0.5830582676194922 1105 39 -0.7181603290975289 1105 40 -0.758520965357917 1105 41 -0.846545468898583 1105 42 -0.8974543790892966 1105 43 -0.85235358913814 1105 44 -0.6936077444116933 1105 45 -0.6686611971948011 1105 46 -0.7252725901815893 1105 47 -0.5739117665491023 1105 48 -0.8554714034237172 1105 49 -1.0164824841688542 1105 50 -0.8616288335120892 1105 51 -1.0410505902433371 1105 52 -1.1615602472784268 1105 53 -1.051163388025028 1105 54 -1.0022243482449262 1105 55 -0.9454422478463975 1105 56 -0.9294733186165232 1105 57 -0.9544601278443352 1105 58 -0.6087365397125275 1105 59 -0.8280506839369437 1105 60 -0.5992884524766637 1105 61 -0.9120776626991685 1105 62 -0.7610119152780541 1105 63 -0.7851113607697897 1105 64 -0.9161230873850705 1105 65 -0.8674253876223403 1105 66 -0.6869867415197177 1105 67 -0.7771819414596344 1105 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-2.131189099202956 2216 43 -2.534880848549669 2216 44 -2.2574854487433385 2216 45 -2.6040809874175643 2216 46 -2.744593847336297 2216 47 -2.01768461369209 2216 48 -2.278738006597724 2216 49 -1.745073355684223 2216 50 -1.8430242262079197 2216 51 -1.6505141401117953 2216 52 -1.572128318013025 2216 53 -2.6242726739840343 2216 54 -2.0289949920039447 2216 55 -2.0140363254268863 2216 56 -1.6492978022671636 2216 57 -1.6184582167780661 2216 58 -1.7847809468576514 2216 59 -1.9443365517904816 2216 60 -1.771284416606452 2216 61 -1.4646807046891936 2216 62 -1.6328793224829 2216 63 -1.5169271566125921 2216 64 -1.8331614375816798 2216 65 -1.6568214843952163 2216 66 -1.5586686998597301 2216 67 -2.3582026645844785 2216 68 -2.402452105154399 2216 69 -1.6950127751168083 2216 70 -2.151989184326883 2216 71 -1.8871909404915606 2216 72 -2.2575343306592117 2216 73 -2.0077435258969665 2216 74 -2.3819892823038593 2216 75 -2.1630481667177435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20071.0 3 2198.0 4 1479.0 5 1263.0 6 1190.0 7 1028.0 8 1114.0 9 1490.0 10 1645.0 11 1529.0 12 1395.0 13 1311.0 14 1224.0 15 1310.0 16 1506.0 17 1494.0 18 1518.0 19 1574.0 20 1813.0 21 2507.0 22 3866.0 23 5209.0 24 5160.0 25 4964.0 26 3955.0 27 3277.0 28 3746.0 29 4562.0 30 4948.0 31 5191.0 32 6405.0 33 8181.0 34 10584.0 35 14578.0 36 19802.0 37 28009.0 38 50684.0 39 98949.0 40 19018.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.571192890861106 23.026644974796067 14.79355076669707 20.608611367645754 2 13.687322550300646 24.912007822797623 40.72572459520739 20.67494503169434 3 15.846025841536882 33.76955342004363 29.420409610375497 20.964011128043985 4 10.495872730859736 20.79274446957372 42.25625952474218 26.45512327482437 5 10.5459088998619 42.75118871641501 34.814022707843094 11.888879675879993 6 24.142022662095744 45.300745967799585 19.026038822348728 11.531192547755948 7 24.65973945764185 36.73412784646529 24.320050781741447 14.286081914151408 8 23.910687684244536 37.24548877877503 21.47773053619985 17.366093000780584 9 26.697584253760574 19.698524933737815 21.002324537451358 32.60156627505025 10 13.313337927130183 33.23831226572351 34.04032057458677 19.40802923255954 11 28.110033824450248 31.02928688451938 25.348037295530766 15.512641995499605 12 21.231486246926288 32.34431291816778 29.858180362555043 16.56602047235089 13 24.81450877348483 28.41196636425759 27.37664654736138 19.396878314896195 14 20.921980746082166 27.13361372649372 29.49389129856725 22.45051422885686 15 21.078982206629554 35.32740316294816 24.64566231196585 18.94795231845644 16 19.89214876151209 33.84504533354683 27.401133982003827 18.86167192293726 17 17.455452112450534 34.43683235354668 27.667726513713202 20.43998902028959 18 17.589722768653644 33.32866325083489 30.724186833798434 18.35742714671302 19 19.400236748651846 32.29646192292516 30.978046423322603 17.32525490510039 20 19.619174525469383 32.209176641484184 30.798881371635105 17.372767461411325 21 20.856494189770338 31.36265898069357 29.922682770610304 17.858164058925794 22 19.617576497568034 31.214381914519457 30.555563498485917 18.61247808942659 23 19.88019329196809 32.02813599062134 29.881337031424245 18.21033368598633 24 19.313772307833084 31.927688449539477 30.310915647009374 18.447623595618072 25 19.637592682082943 31.956410469036395 30.58044075638302 17.82555609249765 26 18.81212467829568 31.65456105233057 31.503002573634543 18.030311695739204 27 18.98177139180785 32.38687540210165 29.945242690685536 18.68611051540496 28 18.965753894722393 30.68194816187404 31.55468624894196 18.7976116944616 29 19.05362397165676 31.649667012664672 31.13810467440444 18.15860434127413 30 19.041246311166017 32.28044288884313 30.171516482510924 18.506794317479926 31 19.91936175688428 31.00511852677933 30.975093649023478 18.100426067312917 32 18.758757556520198 31.940131198920213 31.034778580375068 18.266332664184525 33 19.18510214820068 32.00538765985679 30.48202969504244 18.327480496900094 34 19.500052899916216 30.62704987175345 31.444568036440895 18.428329191889443 35 19.031442656326846 32.56699722060186 30.29572567454735 18.105834448523943 36 19.06160286397273 31.397330466310574 29.940753279729158 19.600313389987534 37 19.71582493122952 30.570980858644493 30.946430511789636 18.766763698336355 38 18.34327479025718 32.02874348753553 31.77939687859222 17.84858484361507 39 19.123651793986117 32.09902666102412 30.12547037092107 18.651851174068696 40 18.875056470198032 31.253037953691575 32.05505772826827 17.816847847842123 41 18.911840058559797 31.42013221703724 31.265440570958003 18.402587153444962 42 18.870670723338062 31.698940842750606 30.35183236491742 19.078556068993915 43 18.264611689351483 31.444012352739335 30.806645316253 19.48473064165618 44 18.2109850391179 32.14473624010614 30.890275426636777 18.754003294139178 45 19.44210941654801 31.025944542610922 30.169608801816178 19.362337239024892 46 18.546560834457992 31.106892055722028 30.480133586476654 19.866413523343322 47 19.41603501959988 32.00313369378436 29.984245754788464 18.59658553182729 48 18.202300520090237 32.281906635367854 30.025704294818826 19.490088549723087 49 19.180722064806847 31.625146177093725 30.173811355065233 19.020320403034194 50 18.906809779640714 31.003553997603984 30.05801336394594 20.031622858809367 51 19.233903364432003 32.307639522283246 28.89517279633564 19.563284316949108 52 18.95312897608843 31.672609057404355 29.650575987785455 19.72368597872176 53 18.991156464530075 32.03001026456267 29.548788123986768 19.430045146920488 54 18.93254266655611 32.25388638072664 29.41097421850652 19.402596734210732 55 19.78887595890744 31.126500012866444 29.470159858411936 19.61446416981418 56 19.3325460976077 32.299061893311446 28.927767786428475 19.440624222652374 57 19.495521048071893 31.55538146145642 28.789953880948225 20.159143609523454 58 18.948554240636803 30.79854866517797 29.795823838374595 20.457073255810627 59 19.41374765187407 31.917643325032095 29.159077847701337 19.509531175392496 60 19.102379720197742 32.72794334190144 28.674727731760385 19.49494920614044 61 20.445350496215838 32.058602361135335 28.642704583599 18.85334255904983 62 19.166997858451968 32.183835744123606 29.654007039374175 18.99515935805025 63 19.15813430851444 31.88218912528199 29.603113107474833 19.356563458728736 64 19.729404398036294 30.924639811063425 29.15707640094125 20.188879389959027 65 19.035474214217707 31.965963968239897 29.522483395139915 19.47607842240248 66 19.72111269003022 31.912496747649016 28.355639934009442 20.01075062831132 67 19.454062508041527 31.94280437001604 28.77194086010745 19.831192261834985 68 19.382868187575593 31.662315902638195 29.75522306124141 19.199592848544807 69 19.650204290530013 32.807715291339164 27.97021847220991 19.571861945920908 70 20.185448338370307 31.239724715294198 29.35893660274427 19.215890343591223 71 19.930120915976406 31.97254015045161 29.113902335116528 18.983436598455455 72 20.050779563513053 32.96125484993438 27.73347591258824 19.254489673964322 73 19.750562549500067 31.26517168124387 29.159649689632793 19.82461607962327 74 19.550989715422862 32.76797227710316 27.946487032054602 19.734550975419374 75 19.741984920528267 32.466039737295816 28.610967356403343 19.18100798577257 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1549.0 1 1367.0 2 1438.5 3 1648.0 4 1604.0 5 1319.0 6 893.5 7 703.0 8 653.0 9 875.0 10 1309.0 11 1357.0 12 1193.0 13 1027.0 14 822.5 15 867.0 16 950.0 17 986.5 18 1143.5 19 1419.0 20 1574.0 21 1818.5 22 2176.5 23 2580.0 24 2870.0 25 3288.5 26 4125.0 27 5553.0 28 6563.0 29 7485.5 30 9457.0 31 11641.5 32 12777.0 33 15392.5 34 20161.5 35 23339.0 36 24363.0 37 24538.5 38 23309.5 39 21158.5 40 20412.0 41 20249.5 42 18621.0 43 16362.0 44 15569.0 45 14922.0 46 13346.0 47 12468.0 48 12519.0 49 11497.5 50 9176.5 51 7255.5 52 6634.0 53 6177.0 54 5235.5 55 4348.0 56 3945.0 57 3591.0 58 3008.5 59 2553.0 60 2326.0 61 1995.5 62 1422.5 63 977.0 64 774.0 65 690.5 66 506.5 67 345.5 68 285.0 69 272.0 70 215.5 71 146.0 72 120.0 73 105.5 74 87.0 75 66.5 76 50.0 77 43.0 78 31.5 79 24.0 80 21.0 81 16.5 82 9.0 83 3.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0042888144858998075 8 0.0022873677258132305 9 0.0 10 0.0 11 0.0 12 0.0020014467600865766 13 0.0 14 0.0 15 0.0028592096572665386 16 0.001715525794359923 17 0.003145130622993192 18 8.577628971799615E-4 19 0.0025732886915398843 20 0.009435391868979577 21 0.005432498348806423 22 0.008577628971799615 23 0.004860656417353115 24 0.010864996697612846 25 0.008577628971799615 26 0.013438285389152732 27 0.006290261245986384 28 0.012294601526246117 29 0.00972131283470623 30 0.012294601526246117 31 0.010579075731886193 32 0.014010127320606037 33 0.016869336977872577 34 0.008577628971799615 35 0.008863549937526269 36 0.006576182211713038 37 0.009435391868979577 38 0.009435391868979577 39 0.006576182211713038 40 0.0014296048286332693 41 0.005432498348806423 42 0.010007233800432884 43 0.007719866074619654 44 0.010007233800432884 45 2.859209657266538E-4 46 0.003145130622993192 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 349747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.029985160468634 #Duplication Level Percentage of deduplicated Percentage of total 1 86.59725859456383 47.65445855396111 2 7.073008578254665 7.784551142024432 3 2.176639028136619 3.5934124025416505 4 1.0075767070189157 2.2178772494113916 5 0.571065310571016 1.571285778319071 6 0.3627925621483988 1.1978681586812867 7 0.2618182699028769 1.008549885524642 8 0.20132433379970227 0.8863100081151081 9 0.1668631181419709 0.8264227423663966 >10 1.2817192045873522 15.106206975516617 >50 0.19960965071729314 7.6133566783906605 >100 0.09720574136493176 9.235575191377825 >500 0.002599083993714753 0.8927779896020007 >1k 5.198167987429507E-4 0.4113472441677914 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1438 0.41115434871492823 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 897 0.25647110625680847 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA 623 0.17812876164770533 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC 579 0.16554823915573255 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG 520 0.14867890217786 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGCTGGTAGAATAGTTCAAATGGTT 502 0.14353232479478023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 484 0.13838574741170045 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC 464 0.1326673280971674 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAGGGCACCAATGATACTGAAGCTACGA 458 0.13095180230280745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 440 0.1258052249197277 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGCCTTATAGAAGGTAAACG 427 0.12208825236528119 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGG 427 0.12208825236528119 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT 417 0.11922904270801465 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAG 385 0.11007957180476173 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGAGCTCATGTAA 370 0.10579075731886192 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 362 0.10350338959304868 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTAG 360 0.10293154766159537 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 350 0.10007233800432885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8592096572665386E-4 0.0 5 0.0 0.0 0.0 2.8592096572665386E-4 0.0 6 0.0 0.0 0.0 2.8592096572665386E-4 0.0 7 0.0 0.0 0.0 2.8592096572665386E-4 0.0 8 0.0 0.0 0.0 5.718419314533077E-4 0.0 9 0.0 0.0 0.0 8.577628971799615E-4 0.0 10 0.0 0.0 0.0 8.577628971799615E-4 0.0 11 0.0 0.0 0.0 8.577628971799615E-4 0.0 12 0.0 0.0 0.0 8.577628971799615E-4 0.0 13 0.0 0.0 0.0 8.577628971799615E-4 0.0 14 0.0 0.0 0.0 8.577628971799615E-4 0.0 15 0.0 0.0 0.0 8.577628971799615E-4 0.0 16 0.0 0.0 0.0 0.001715525794359923 0.0 17 0.0 0.0 0.0 0.001715525794359923 0.0 18 0.0 0.0 0.0 0.001715525794359923 0.0 19 0.0 0.0 0.0 0.001715525794359923 0.0 20 0.0 0.0 0.0 0.001715525794359923 0.0 21 0.0 0.0 0.0 0.0025732886915398843 0.0 22 0.0 0.0 0.0 0.004002893520173154 0.0 23 0.0 0.0 0.0 0.007148024143166346 0.0 24 0.0 0.0 0.0 0.012294601526246115 0.0 25 0.0 0.0 0.0 0.01343828538915273 0.0 26 0.0 0.0 0.0 0.018870783737959154 0.0 27 0.0 0.0 0.0 0.022873677258132306 0.0 28 0.0 0.0 0.0 0.02916393850411869 0.0 29 0.0 0.0 0.0 0.04574735451626461 0.0 30 0.0 0.0 0.0 0.07462537205465665 0.0 31 0.0 0.0 0.0 0.11093733470194168 0.0 32 0.0 0.0 0.0 0.1466774554177734 0.0 33 0.0 0.0 0.0 0.1821316551678785 0.0 34 0.0 0.0 0.0 0.22416203712969662 0.0 35 0.0 0.0 0.0 0.268479786817328 0.0 36 0.0 0.0 0.0 0.31622858809367915 0.0 37 0.0 0.0 0.0 0.3965723794628689 0.0 38 0.0 0.0 0.0 0.475772486969152 0.0 39 0.0 0.0 0.0 0.5664094331045012 0.0 40 0.0 0.0 0.0 0.6722001904233632 0.0 41 0.0 0.0 0.0 0.7625512155929858 0.0 42 0.0 0.0 0.0 0.8631953955287679 0.0 43 0.0 0.0 0.0 0.9595507609786503 0.0 44 0.0 0.0 0.0 1.0607667828458858 0.0 45 0.0 0.0 0.0 1.166271619199021 0.0 46 0.0 0.0 0.0 1.2812118474211358 0.0 47 0.0 0.0 0.0 1.4004408901291505 0.0 48 0.0 0.0 0.0 1.5245305892545182 0.0 49 0.0 0.0 0.0 1.6360397658879133 0.0 50 0.0 0.0 0.0 1.746119337692675 0.0 51 0.0 0.0 0.0 1.8702090368180428 0.0 52 0.0 0.0 0.0 1.9851492650401577 0.0 53 0.0 0.0 0.0 2.087223049804573 0.0 54 0.0 0.0 0.0 2.2181748521073805 0.0 55 0.0 0.0 0.0 2.3436941560613813 0.0 56 0.0 0.0 0.0 2.4735022745012825 0.0 57 0.0 0.0 0.0 2.5933031591407505 0.0 58 0.0 0.0 0.0 2.724826803375011 0.0 59 0.0 0.0 0.0 2.8669295233411582 0.0 60 0.0 0.0 0.0 3.006458954615765 0.0 61 0.0 0.0 0.0 3.142557334301652 0.0 62 0.0 0.0 0.0 3.2886629477879725 0.0 63 0.0 0.0 0.0 3.435054482240019 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGATG 15 0.0023531667 69.00744 31 TATCGAA 20 0.007351642 51.755577 23 ATGCGTA 170 0.0 32.469444 13 CTACGGC 55 3.2503754E-4 31.367016 34 CCGAATA 55 3.2531028E-4 31.362534 51 CAACGAC 45 0.0046159043 30.669971 19 CTCGTAA 45 0.004619135 30.665588 40 AAGGTAG 45 0.004619135 30.665588 6 CGGCCTG 125 1.2369128E-10 30.35893 44 TTAGTCG 115 1.6952981E-9 29.998941 39 GTCGGCC 115 1.6952981E-9 29.998941 42 TGCGTAG 185 0.0 29.836786 14 CGGATAA 70 3.8443657E-5 29.570387 56 ATATAGG 295 0.0 29.236258 2 GTATCAA 1965 0.0 28.968445 1 GCTACGG 60 5.4111704E-4 28.7531 33 TAAGTCC 120 2.6720954E-9 28.748987 66 CGAATAG 60 5.4157013E-4 28.748987 52 TCGGCCT 135 3.0559022E-10 28.11012 43 TAGGGCG 50 0.007723619 27.602972 31 >>END_MODULE