FastQCFastQC Report
Mon 6 Feb 2017
SRR2079282_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079282_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5874233
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93550.039.7170641
GGTATCA41100.031.141341
TCAACGC122950.029.29394
ATCAACG123250.029.2785763
CAACGCA125150.028.7789465
TATCAAC126550.028.760442
AACGCAG131750.027.3896396
ACGCAGA150600.023.7094027
CGCAGAG155550.022.821068
AGAGTAC154650.021.1921611
CAGAGTA170050.019.86095610
GAGTACT81050.019.75030912
GTACATG163550.019.638321
GCAGAGT175800.019.3094719
TACATGG165800.018.685172
AGTACTT90450.017.77404413
ACATGGG171050.017.728463
GTACTTT91150.017.14667914
GCGATAC5400.015.97293614
GTATTAG14300.015.6813161
Produced by FastQC (version 0.11.5)