FastQCFastQC Report
Mon 6 Feb 2017
SRR2079282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5874233
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122050.043.1212581
GGTATCA47850.037.625741
TATCAAC170400.030.5613782
ATCAACG171200.030.337833
TCAACGC172300.030.057774
CAACGCA174500.029.6577555
AACGCAG181950.028.4580296
ACGCAGA206350.024.9571557
CGCAGAG211850.024.229548
AGAGTAC212150.023.08663211
GAGTACT141600.023.01885812
CAGAGTA226300.021.97839210
AGTACTT153050.021.45452513
GCAGAGT236200.021.320059
GTACTTT157600.020.3755214
GTACATG160300.018.6471231
TACTTTT177700.018.14092415
TACATGG161900.017.9292342
ACTTTTT189000.017.3850216
ACATGGG166250.016.9196763
Produced by FastQC (version 0.11.5)