FastQCFastQC Report
Mon 6 Feb 2017
SRR2079281_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079281_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3889682
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76750.038.566221
GGTATCA34350.029.8282531
TCAACGC101850.028.2828754
ATCAACG102650.028.1632773
TATCAAC106400.027.4949132
CAACGCA106650.027.0422955
AACGCAG110900.025.8815276
ACGCAGA126300.022.4252837
TATACCG1854.8567017E-1022.3772965
CGCAGAG129200.021.761728
AGAGTAC121450.021.16282511
GTACATG139100.020.3865071
TACATGG140900.019.6363832
CAGAGTA135100.019.48351710
GCAGAGT139200.019.4053139
GAGTACT58800.019.24545512
ACATGGG143850.018.7060833
AGTACTT62150.018.65218713
GTACTTT61500.018.17613414
CGTCGTT2301.4260695E-716.49920546
Produced by FastQC (version 0.11.5)