FastQCFastQC Report
Mon 6 Feb 2017
SRR2079281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3889682
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112950.041.694591
GGTATCA43750.034.3543171
TATCAAC156850.029.9147512
ATCAACG157250.029.772743
TCAACGC157750.029.6953054
CAACGCA160950.029.0386435
AACGCAG167300.027.892656
GGCGAAT10750.026.3103632
TATGCCG11300.024.41928743
ACGCAGA189300.024.3030977
TCGTATG12200.023.74875840
GAGTACT122850.023.72482112
CGCAGAG193850.023.6840528
AGAGTAC185550.023.49673711
AGTACTT127200.022.83212913
CAGAGTA201100.022.10865410
GTACTTT130350.022.06868414
CTCGTAT11250.021.76843639
GCAGAGT207100.021.767929
AGGCGAA14650.020.95433231
Produced by FastQC (version 0.11.5)