FastQCFastQC Report
Mon 6 Feb 2017
SRR2079280_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079280_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5423848
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90850.040.1761251
GGTATCA37500.033.11911
TCAACGC121500.029.7856124
ATCAACG123350.029.4507623
TATCAAC124950.029.0460322
CAACGCA126200.028.6216475
AACGCAG132750.027.1314686
ACGCAGA154200.023.0888677
CGCAGAG162150.021.8498678
GAGTACT69700.021.82836212
AGAGTAC157800.021.05302811
GTACATG181400.020.4636121
AGTACTT78900.020.02644213
GTACTTT77450.019.68938614
CAGAGTA173150.019.62497110
TACATGG185350.019.3760592
GCAGAGT177650.019.283219
ACATGGG189350.018.4019323
CGCGTAA1250.001942816716.55878847
CATGGGG133950.016.406054
Produced by FastQC (version 0.11.5)